Alanine mother liquor as a nitrogen source for docosahexaenoic acid production by Schizochytrium sp. B4D1

Publication date: Available online 30 June 2018Source: Electronic Journal of BiotechnologyAuthor(s): Jian Xu, Yujing Zhu, Hanchen Li, Limei Chen, Wuxi Chen, Min Cui, Lina Han, Wenbo Hou, Demao LiAbstractAlanine mother liquor, a type of industrial waste from alanine fermentation, was used as a nitrogen source to produce docosahexaenoic acid (DHA) by Schizochytrium sp. B4D1. The results indicated that yeast extract could trigger the utilization of the alanine mother liquor. Additionally, the alanine can be quenched during the culture, which aids in DHA accumulation. The medium components were optimized via response surface methodology as follows: 99.98 g/L glucose, 0.05 g/L yeast extract and a 183.17 dilution factor of the alanine mother liquid (v/v, with an alanine content of 0.72 g/L) and 17.98% inoculum concentration (v/v). Finally, in a 50 mL shake-flask fermentation, the DHA yield was 2.29 g/L.

Source: Electronic Journal of Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research