Diagnostic Microbiology and Infectious Disease This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

An advanced uracil DNA glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP) technique used in the sensitive and specific detection of Cryptosporidium parvum, Cryptosporidium hominis, and Cryptosporidium meleagridis in AIDS patients
This study was conducted to examine a UDG-LAMP technique for the first time to diagnose cryptosporidiosis in APs. After collecting demographic and clinical data, three stool samples were collected from the participants (120 volunteering APs). The microscopic examination of stained smears using the acid-fast method and the UDG-LAMP assay were performed for each sample. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - December 23, 2017 Category: Microbiology Authors: Shirzad Fallahi, Seyedeh Fatemeh Moosavi, Azadeh Karimi, Ali Sharafi Chegeni, Mohammad Saki, Parsa Namdari, Mohammad Menati Rashno, Ali Mohamad Varzi, Mohammad Javad Tarrahi, Mohammad Almasian Source Type: research

Comparison of six commercial tick-borne encephalitis IgM and IgG ELISA kits and the molecular characterization of their antigenic design
Tick-borne encephalitis virus (TBEV) diagnosis is mainly based on the detection of viral-specific antibodies in serum. Several commercial assays are available, but published data on their performance remain unclear. We assessed six IgM and six IgG commercial ELISA kits (ELISA-1 through ELISA-6) using 94 samples, including pre-characterized TBEV positive samples (n=50) and negative samples (n=44). The six manufacturers showed satisfactory sensitivity and specificity and high overall agreement for both IgM and IgG. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - December 22, 2017 Category: Microbiology Authors: Aur élie Velay, Morgane Solis, Heidi Barth, Véronique Sohn, Anne Moncollin, Amandine Neeb, Marie-Josée Wendling, Samira Fafi-Kremer Source Type: research

Survey of diagnostic testing for respiratory syncytial virus (RSV) in adults: infectious disease physician practices and implications for burden estimates
Respiratory syncytial virus (RSV) often causes respiratory illness in adults. Over 40 RSV vaccine and monoclonal antibody products are currently in preclinical development or clinical trials. Because RSV diagnostic practices may impact disease burden estimates, we investigated infectious disease physicians' RSV diagnostic practices among their adult patients. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - December 21, 2017 Category: Microbiology Authors: Kristen E. Allen, Susan E. Beekmann, Philip Polgreen, Sarah Poser, Jeanette St. Pierre, Scott Santiba ñez, Susan I. Gerber, Lindsay Kim Source Type: research

In vitro activity of eravacycline against 2213 Gram-negative and 2424 Gram-positive bacterial pathogens isolated in Canadian hospital laboratories: CANWARD surveillance study 2014 –2015
Gram-negative (n=2213) and Gram-positive (n=2424) pathogens isolated from patients in 13 Canadian hospitals in 2014 and 2015 were tested for in vitro susceptibility to eravacycline and comparators using the Clinical and Laboratory Standards Institute broth microdilution method. The concentration of eravacycline inhibiting 90% of isolates (MIC90) ranged from 0.5 to 2 μg/mL for 9 species of Enterobacteriaceae tested (n=2067). Eravacycline activity was largely unaffected by extended-spectrum β-lactamase phenotypes in Escherichia coli (n=141) and Klebsiella pneumoniae (n=21). (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - December 21, 2017 Category: Microbiology Authors: George G. Zhanel, Melanie R. Baxter, Heather J. Adam, Joyce Sutcliffe, James A. Karlowsky Tags: Antimicrobial Susceptibility Studies Source Type: research

Survey of diagnostic testing for respiratory syncytial virus (RSV) in adults: infectious disease physician practices and implications for burden estimates
Respiratory syncytial virus (RSV) often causes respiratory illness in adults. Over 40 RSV vaccine and monoclonal antibody products are currently in preclinical development or clinical trials. Because RSV diagnostic practices may impact disease burden estimates, we investigated infectious disease physicians' RSV diagnostic practices among their adult patients. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - December 21, 2017 Category: Microbiology Authors: Kristen E. Allen, Susan E. Beekmann, Philip Polgreen, Sarah Poser, Jeanette St. Pierre, Scott Santiba ñez, Susan I. Gerber, Lindsay Kim Source Type: research

In Vitro Activity of Eravacycline against 2213 Gram-Negative and 2424 Gram-Positive Bacterial Pathogens Isolated in Canadian Hospital Laboratories: CANWARD Surveillance Study 2014 –2015
Gram-negative (n=2213) and Gram-positive (n=2424) pathogens isolated from patients in 13 Canadian hospitals in 2014 and 2015 were tested for in vitro susceptibility to eravacycline, and comparators using the Clinical and Laboratory Standards Institute CLSI broth microdilution method. The concentration of eravacycline inhibiting 90% of isolates (MIC90) ranged from 0.5 to 2 μg/mL for 9 species of Enterobacteriaceae tested (n=2067). Eravacycline activity was largely unaffected by ESBL phenotypes in Escherichia coli (n=141) and Klebsiella pneumoniae (n=21). (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - December 21, 2017 Category: Microbiology Authors: George G. Zhanel, Melanie R. Baxter, Heather J. Adam, Joyce Sutcliffe, James A. Karlowsky Source Type: research

Sanger sequencing versus INNO-LiPA ® HBV PreCore assay for routine detection of precore and basal core promoter mutations in Hepatitis virus B chronically-infected patients
We compared the Sanger sequencing and the commercial INNO-LiPA ® HBV assay for the routine detection of Precore (PC) and basal core promoter (BCP) mutations of hepatitis B virus in chronically-infected patients. The overall agreement rate between assays was 94.2% and 98.8% for the detection of PC and BCP mutations respectively. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - December 17, 2017 Category: Microbiology Authors: EK Alidjinou, L Bocket, V Pigot, V Lambert, C Hallaert, V Canva, D Hober Tags: Note Source Type: research

Comparison of Cepheid ® Xpert Flu and Roche RealTime Ready Influenza A/H1N1 Detection Set for detection of influenza A/H1N1
To compare two influenza polymerase chain reaction (PCR) methods. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - December 16, 2017 Category: Microbiology Authors: Ali A. Rabaan, Ali M. Bazzi, Sana A. Alshaikh Source Type: research

Treatment outcomes of macrolide-susceptible Mycobacterium abscessus lung disease
Mycobacterium abscessus lung disease is difficult to treat due to inducible resistance to macrolides. However, 15% –20% of isolates are macrolide susceptible. In 14 patients with macrolide-susceptible M. abscessus lung disease, all isolates had nonfunctional erm(41) gene, and sputum culture conversion rate was achieved in 93% (13/14) following antibiotic therapy. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - December 16, 2017 Category: Microbiology Authors: Hayoung Choi, Byung Woo Jhun, Su-Young Kim, Dae Hun Kim, Hyun Lee, Kyeongman Jeon, O Jung Kwon, Hee Jae Huh, Chang-Seok Ki, Nam Yong Lee, Sung Jae Shin, Charles L. Daley, Won-Jung Koh Tags: Note Source Type: research

Rapid Detection of blaKPC directly from surveillance rectal swabs by EasyQ KPC
The performance of EasyQ KPC assay was evaluated for the first time for blaKPC detection directly from surveillance rectal swabs without broth enrichment. Using conventional PCR as gold standard method, EasyQ KPC and culture-based molecular tests demonstrated a sensitivity/specificity of 100%/87.3% and 83.3%/98.2%, respectively. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - December 16, 2017 Category: Microbiology Authors: Ana Carolina Ramos, Lygia Schandert, Cecilia G. Carvalhaes, Rafael Affini Martins, Paola Cappellano, Fl ávia Ribeiro Machado, Ana Cristina Gales Source Type: research

Comparison of Cepheid ® Xpert Flu and Roche Real-Time Ready Influenza A/H1N1 Detection Set for Detection of Influenza A/H1N1
To compare two influenza PCR methods. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - December 16, 2017 Category: Microbiology Authors: Ali A. Rabaan, Ali M. Bazzi, Sana A. Alshaikh Source Type: research

Detection of “Xisco” gene for identification of Streptococcus pneumoniae isolates
We describe a PCR-assay differentiating Streptococcus pneumoniae from closely-related species of the Mitis group of the genus Streptococcus and identification of pneumococcus clinical isolates, based on the “Xisco” gene discriminatory marker. The complete “Xisco” gene sequence was observed in all S. pneumoniae genomes analyzed and absent in all non-pneumococcus genomes. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - December 15, 2017 Category: Microbiology Authors: Francisco Salv à-Serra, Gwendolyn Connolly, Edward R.B. Moore, Lucia Gonzales-Siles Tags: Note Source Type: research

Impact of Clinical and Laboratory Standards Institute breakpoint changes on susceptibility rates of cephalosporins in uncomplicated urinary tract infections caused by Enterobacteriaceae
Breakpoint changes may impact cephalosporin susceptibility rates in uncomplicated urinary tract infections (uUTI). Applying the ≤16mg/L breakpoint to urine cultures from adult women in an academic health system resulted in cefazolin being the most active uUTI antimicrobial, with 86.9% susceptibility, compared to levofloxacin (80%), nitrofurantoin (76.5%), and sulfamethoxazole-trimethoprim (72.6%). (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - December 15, 2017 Category: Microbiology Authors: Kristen L. Bunnell, Eric Wenzler, Amanda Harrington, Larry H. Danziger Tags: Note Source Type: research

Real-time RT-PCR, a necessary tool to support the diagnosis and surveillance of rotavirus in Mexico
In this study a real-time RT-PCR assay was tested using a panel of 440 samples from patients with acute gastroenteritis, and characterized by PAGE and RT-PCR. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - December 14, 2017 Category: Microbiology Authors: Sergio Isaac De La Cruz Hern ández, Yazmin Anaya Molina, Fabián Gómez Santiago, Heidi Lizbeth Terán Vega, Elda Monroy Leyva, Héctor Méndez Pérez, Herlinda García Lozano Source Type: research

Real time RT-PCR, a necessary tool to support the diagnosis and surveillance of rotavirus in Mexico
In this study a real time RT-PCR assay was tested using a panel of 440 samples from patients with acute gastroenteritis, and characterized by PAGE and RT-PCR. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - December 14, 2017 Category: Microbiology Authors: Sergio Isaac De La Cruz Hern ández, Yazmin Anaya Molina, Fabián Gómez Santiago, Heidi Lizbeth Terán Vega, Elda Monroy Leyva, Héctor Méndez Pérez, Herlinda García Lozano Source Type: research