Comparison of six commercial tick-borne encephalitis IgM and IgG ELISA kits and the molecular characterization of their antigenic design

Tick-borne encephalitis virus (TBEV) diagnosis is mainly based on the detection of viral-specific antibodies in serum. Several commercial assays are available, but published data on their performance remain unclear. We assessed six IgM and six IgG commercial ELISA kits (ELISA-1 through ELISA-6) using 94 samples, including pre-characterized TBEV positive samples (n=50) and negative samples (n=44). The six manufacturers showed satisfactory sensitivity and specificity and high overall agreement for both IgM and IgG.
Source: Diagnostic Microbiology and Infectious Disease - Category: Microbiology Authors: Source Type: research