Diagnostic Microbiology and Infectious Disease This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

A culture medium for screening 16S rRNA methylase-producing pan-aminoglycoside resistant Gram-negative bacteria
The amikacin plus gentamicin-containing SuperAminoglycoside medium was developed for screening multiple-aminoglycoside resistance in Gram-negative bacteria (Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter baumannii). It was evaluated using aminoglycoside-susceptible (n=12) and aminoglycoside-resistant (n=59) Gram-negatives isolates, including 16S rRNA methylase producers (n=20). Its sensitivity and specificity of detection were respectively of 95% and 96% for detecting multiple aminoglycoside-resistant methylase producers, respectively. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 3, 2018 Category: Microbiology Authors: Patrice Nordmann, Alain Maz é, Esther Culebras, Jan Dobias, Aurélie Jayol, Laurent Poirel Source Type: research

Prolonged linezolid use is associated with the development of linezolid-resistant Enterococcus faecium
We assessed risk factors for and outcomes of linezolid-resistant vancomycin-resistant Enterococcus faecium (LRVREF) bacteremia over 7-years. Thirty-four LRVREF cases were matched to 68 linezolid-susceptible VREF (LSVREF) controls. The odds of bacteremia with LRVREF increased by 7% for each additional day of prior linezolid exposure. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 3, 2018 Category: Microbiology Authors: Tiffeny T. Smith, Pranita D. Tamma, Tiffany B. Do, Kathryn E. Dzintars, Yuan Zhao, Sara E. Cosgrove, Edina Avdic Source Type: research

Oritavancin in vitro activity against gram-positive organisms from European and United States medical centers: Results from the SENTRY Antimicrobial Surveillance Program for 2010-2014
The in vitro activity of oritavancin was assessed against 44,715 gram-positive pathogens causing infections in European and United States (US) hospitals (2010-2014). There were no substantive differences (>±2-fold dilution) in oritavancin MIC50 or MIC90 values for different species/organism groups over time or by region. Oritavancin (99.9% susceptible) showed modal MIC, MIC50 and MIC90 results of 0.03, 0.03 and 0.06-0.12 mg/L when tested against Staphylococcus aureus, regardless of methicillin suscep tibility, year, or region. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 3, 2018 Category: Microbiology Authors: M.A. Pfaller, H.S. Sader, R.K. Flamm, M. Castanheira, Rodrigo E. Mendes Source Type: research

Colistin-resistant KPC-2-producing Klebsiella pneumoniae ST423 harboring an IS5-like element in the mgrB gene isolated from cerebrospinal fluid
We describe colistin-resistant KPC-2-producing K. pneumoniae isolates from cerebrospinal fluid, belonging to ST423, selected during treatment for neuroinfection. Colistin resistance was related to mgrB gene interruption by an IS5-like. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 3, 2018 Category: Microbiology Authors: Hemilly Rayanne Ferreira da Silva, Marinalda Anselmo Vilela, Anna Carolina Soares Almeida, M árcia Maria Camargo de Morais Tags: Note Source Type: research

Amikacin and cefoperazone/sulbactam alone or in combination against carbapenem-resistant Pseudomonas aeruginosa
Background: Carbapenem-resistant Pseudomonas aeruginosa (CRPA) has become a major therapeutic problem worldwide. Combination therapy may be an alternative chemotherapeutic option. Methods: Bioluminescent CRPA Xen5-D9 was developed in the present study. Minimum inhibitory concentration (MIC) of amikacin (AMK) or cefoperazone/sulbactam (SCF) against CRPA Xen5-D9 was determined by microdilution method. CRPA Xen5-D9 was intraperitoneally injected to establish a mouse model of intraperitoneal infection. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 3, 2018 Category: Microbiology Authors: Yun Cai, Deqing Yang, Jin Wang, Rui Wang Source Type: research

Persistence of bacterial DNA in orthopedic infections
Polymerase chain reaction (PCR) has been proposed as a method to identify bacteria in clinical samples because it is more sensitive than culture techniques and can produce results rapidly. However, PCR can detect DNA from dead cells and thus cannot distinguish between live and dead cells in a tissue sample. Killed Staphylococcus aureus cells were implanted into the femurs and knee joints of rats to determine the length of time that DNA from dead cells is detectable in a living animal under conditions similar to common orthopedic infections. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 2, 2018 Category: Microbiology Authors: Heidi B. Kaplan, Justin A. Miranda, Gloria R. Gogola, Karen Gomez, Catherine G. Ambrose Source Type: research

Are pathogenic intestinal bacteria present in stool specimens from patients with chronic heart failure?
It has been reported that patients with chronic heart failure exhibit an intestinal overgrowth of primary gut bacterial pathogens, such as Shigella spp., Salmonella spp., Campylobacter spp., and Yersinia enterocolitica. We failed to reproduce these findings in a cohort of 39 patients admitted to the hospital with decompensated heart failure by means of conventional stool bacterial cultures and a multiplexed polymerase chain reaction assay. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 2, 2018 Category: Microbiology Authors: Dixie Huntley, Anna Mollar, Javier Buesa, Silvia Rodr íguez, Estela Giménez, Juan Sanchis, Julio Núñez, David Navarro Tags: Note Source Type: research

The Verigene dilemma: gram-negative polymicrobial bloodstream infections and clinical decision making
Verigene Blood-Culture Gram-Negative (GN) results in rapid identification of key GNs in bloodstream infections. Its use clinically is limited by low sensitivity in polymicrobial GN infections and concerns for inappropriate antibiotic modification. In a retrospective review of 1003 blood culture sets, the incidence of missed GNs was infrequent, (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 2, 2018 Category: Microbiology Authors: Kimberly C. Claeys, Emily L. Heil, Jason M. Pogue, Paul R. Lephart, J. Kristie Johnson Tags: Note Source Type: research

Diagnostic yield of real-time polymerase chain reaction in the diagnosis of intrapartum maternal rectovaginal colonization by group B Streptococcus: a systematic review with meta-analysis
Group B Streptococcus is the leading etiologic factor of neonatal sepsis. Intrapartum real-time polymerase chain reaction (RT-PCR) may allow faster and more accurate detection of maternal colonization. The aim of this study was to determine the sensitivity and specificity of RT-PCR when compared to the reference standard culture in selective broth media collected from rectovaginal tract from laboring women. We selected 15 studies that included 6368 women. Papers selection, data extraction, and quality assessment were carried out by two independent researchers. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 2, 2018 Category: Microbiology Authors: Otto Henrique May Feuerschuette, Sheila Koettker Silveira, Ana Carolina Labor Cancelier, Rosemeri Maurici da Silva, Daisson Jos é Trevisol, Jefferson Ricardo Pereira Source Type: research

Invasive pulmonary aspergillosis and influenza co-infection in immunocompetent hosts: case reports and review of the literature
This report describes two fatal cases of pathologically confirmed IPA in patients with influenza in the intensive care unit. One patient had influenza B infection, whereas the other had influenza A H1N1. Both patients died despite broad-spectrum antimicrobials, mechanical ventilation, and vasopressor support. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 2, 2018 Category: Microbiology Authors: Melisa M. Shah, Eric I. Hsiao, Carl M. Kirsch, Amit Gohil, Supriya Narasimhan, David A. Stevens Tags: Review Source Type: research

Rapid one-step extraction method for the identification of molds using MALDI-TOF MS
In this study, we developed and validated a rapid one-step protein extraction protocol for filamentous fungi. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 2, 2018 Category: Microbiology Authors: Paul M. Luethy, Adrian M. Zelazny Source Type: research

Persistence of bacterial DNA in orthopedic infections
Polymerase chain reaction (PCR) has been proposed as a method to identify bacteria in clinical samples because it is more sensitive than culture techniques and can produce results rapidly. However, PCR can detect DNA from dead cells and thus cannot distinguish between live and dead cells in a tissue sample. Killed Staphylococcus aureus cells were implanted into the femurs and knee joints of rats to determine the length of time that DNA from dead cells is detectable in a living animal under conditions similar to common orthopedic infections. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 2, 2018 Category: Microbiology Authors: Heidi B. Kaplan, Justin A. Miranda, Gloria R. Gogola, Karen Gomez, Catherine G. Ambrose Source Type: research

Persistence of Bacterial DNA in Orthopaedic Infections
Polymerase chain reaction (PCR) has been proposed as a method to identify bacteria in clinical samples because it is more sensitive than culture techniques and can produce results rapidly. However, PCR can detect DNA from dead cells and thus cannot distinguish between live and dead cells in a tissue sample. Killed Staphylococcus aureus cells were implanted into the femurs and knee joints of rats to determine the length of time that DNA from dead cells is detectable in a living animal under conditions similar to common orthopaedic infections. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 2, 2018 Category: Microbiology Authors: Heidi B. Kaplan, Justin A. Miranda, Gloria R. Gogola, Karen Gomez, Catherine G. Ambrose Source Type: research

Are pathogenic intestinal bacteria present in stool specimens from patients with chronic heart failure?
It has been reported that patients with chronic heart failure exhibit an intestinal overgrowth of primary gut bacterial pathogens, such as Shigella spp., Salmonella, spp. Campylobacter spp. and Yersinia enterocolitica. We failed to reproduce these findings in a cohort of 39 patients admitted to the hospital with decompensated heart failure by means of conventional stool bacterial cultures and a multiplexed PCR assay. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 2, 2018 Category: Microbiology Authors: Dixie Huntley, Anna Mollar, Javier Buesa, Silvia Rodr íguez, Estela Giménez, Juan Sanchis, Julio Núñez, David Navarro Tags: Note Source Type: research

The Verigene Dilemma: Gram-negative Polymicrobial Bloodstream Infections and Clinical Decision Making
Verigene Blood-Culture Gram-negative (GN) results in rapid identification of key GNs in bloodstream infections. Its use clinically is limited by low sensitivity in polymicrobial GN infections and concerns for inappropriate antibiotic modification. In a retrospective review of 1,003 blood culture sets, the incidence of missed GNs was infrequent, (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 2, 2018 Category: Microbiology Authors: Kimberly C. Claeys, Emily L. Heil, Jason M. Pogue, Paul R. Lephart, J. Kristie Johnson Tags: Note Source Type: research