Granulomatous inflammation and organizing pneumonia: Role of computed tomography-guided lung fine needle aspirations, touch preparations and core biopsies in the evaluation of common non-neoplastic diagnoses
Discussion: U, N and A FNAs and TPs lacked 3-D clusters seen in carcinomas and were negative on follow-up. Granulomas and organizing pneumonia were the most common specific benign diagnoses, but the latter was recognized on histology only. In the absence of a definitive FNA result at the time of on-site assessment, a CB with a TP containing type II pneumocytes increases the likelihood of a specific benign diagnosis. (Source: CytoJournal)
Source: CytoJournal - January 31, 2014 Category: Pathology Authors: Anjali SaqiShana M ColeyJohn P Crapanzano Source Type: research
Evaluation of a triple combination of cytokeratin 20, p53 and CD44 for improving detection of urothelial carcinoma in urine cytology specimens
This study was designed to determine the utility of a cocktail of three stains, cytokeratin (CK20), p53 and CD44 in urine cytology samples for improving the detection of urothelial carcinoma. Materials and Methods: Urine cytology specimens with cell blocks containing adequate cytologic material between 2005 and 2010 and subsequent follow-up biopsy and/or Urovysion test (102 cases including 29 negative, 56 atypical and 17 malignant) were included in the study and evaluated with the triple stain. Results were first validated on the positive and negative cases and then applied to the atypical cases to determine the utility in...
Source: CytoJournal - December 27, 2013 Category: Pathology Authors: Brent ArvilleEmily O'RourkeFai ChungMahul AminShikha Bose Source Type: research
Clear cell variant of solid pseudopapillary neoplasm of pancreas diagnosed by fine needle aspiration: A case report and review of the literature
We report a case of clear cell variant of SPN, which was diagnosed by fine needle aspiration biopsy. The aspirate was highly cellular and exhibited delicate branching papillary structures with central capillaries covered with several layers of plasmacytoid tumor cells. Acinar and rosette-like formations, as well as single neoplastic cells were also observed. An unusual cytologic feature was the presence of large, clear cytoplasmic vacuoles. The diagnosis of SPN was confirmed by characteristic immunocytochemical staining pattern including nuclear staining for β-catenin, cytoplasmic staining for vimentin and lack...
Source: CytoJournal - December 27, 2013 Category: Pathology Authors: Sule CanberkBilge Baskir ElcinAtay UludokumaciNesrin UygunFatih Gulsen Source Type: research
Protein extraction from methanol fixed paraffin embedded tissue blocks: A new possibility using cell blocks
Conclusion: In the current era of minimally invasive techniques to obtain minimal amount of tissue for diagnostic and prognostic purposes, the use of commercial and lab made buffer on low weight MFPE scrapings obtained by Cellient ® processor opens new possibilities for protein biomarker research. (Source: CytoJournal)
Source: CytoJournal - November 28, 2013 Category: Pathology Authors: Theresa J KokkatDiane McGarveyMiral S PatelAndrew D TieniberVirginia A LiVolsiZubair W Baloch Source Type: research
Pre-analytic steps for molecular testing on thyroid fine-needle aspirations: The goal of good results
In conclusion, the correct use of the pre-analytical-analytical steps might lead to optimal results on cytology and empower the prognostic value of molecular techniques as strong indicators of cancer for their high specificity and positive predictive value. (Source: CytoJournal)
Source: CytoJournal - November 28, 2013 Category: Pathology Authors: Esther Diana RossiFernando Schmitt Source Type: research
Adequacy of fine-needle aspiration specimens for human papillomavirus infection molecular testing in head and neck squamous cell carcinoma
Conclusion: Our study found that HPV molecular testing is not influenced by percent tumor necrosis or method by which FNA was performed. We believe that a portion of the FNA specimen obtained from head and neck lesions diagnosed as SCC during the rapid on-site evaluation should be sent for HPV DNA testing, independent of the amount of tumor necrosis, thus guaranteeing availability of specimen for HPV testing. (Source: CytoJournal)
Source: CytoJournal - October 29, 2013 Category: Pathology Authors: Ricardo R LastraMichelle R PramickMegan O NakashimaGregory S WeinsteinKathleen T MontoneVirginia A LiVolsiZubair W Baloch Source Type: research
Screening and dotting virtual slides: A new challenge for cytotechnologists
Walid E Khalbuss, Jackie Cuda, Ioan C CucoranuCytoJournal 2013 10(1):22-22Digital images are increasingly being used in cytopathology. Whole-slide imaging (WSI) is a digital imaging modality that uses computerized technology to scan and convert entire cytology glass slides into digital images that can be viewed on a digital display using the image viewer software. Digital image acquisition of cytology glass slides has improved significantly over the years due to the use of liquid-based preparations and advances in WSI scanning technology such as automatic multipoint pre-scan focus technology or z-stack scanning technology....
Source: CytoJournal - October 29, 2013 Category: Pathology Authors: Walid E KhalbussJackie CudaIoan C Cucoranu Source Type: research
Can cytomorphology of granulomas distinguish sarcoidosis from tuberculosis? Retrospective study of endobronchial ultrasound guided transbronchial needle aspirate of 49 granulomatous lymph nodes
Conclusions: Different patterns of granulomas observed in cytology smears do not help distinguish TB from sarcoidosis. The novel non-invasive techniques of mediastinal sampling though help in confirming granulomatous pathology, distinction between these entities and treatment decisions still depend upon correlating cytologic, microbiologic, clinical and radiological data in a large number of cases in tubercular endemic regions. (Source: CytoJournal)
Source: CytoJournal - September 27, 2013 Category: Pathology Authors: Gagandeep KaurAmit DhamijaJolsana AugustinePooja BakshiKusum Verma Source Type: research
Bone marrow elements in cerebrospinal fluid: Review of literature with a case study
Anitha Ann Thomas, Felicia Tze Yee GohCytoJournal 2013 10(1):20-20Presence of bone marrow elements in cerebrospinal fluid is rare. Journal publications on this topic are few and majority of them were written over a decade ago mostly as case reports in young children or the elderly. The increased cellularity and presence of myeloid precursors can be a pitfall and may be misdiagnosed as leukemia or lymphoma or central nervous system infection, when the specimen is actually not representative. With the intention to create awareness of potential pitfalls and avoid erroneous diagnoses, as well as adding on to the current photo ...
Source: CytoJournal - September 27, 2013 Category: Pathology Authors: Anitha Ann ThomasFelicia Tze Yee Goh Source Type: research
Can cytomorphology of granulomas distinguish sarcoidosis from tuberculosis ? Retrospective study of endobronchial ultrasound guided transbronchial needle aspirate of 49 granulomatous lymph nodes
Conclusions: Different patterns of granulomas observed in cytology smears do not help distinguish TB from sarcoidosis. The novel non-invasive techniques of mediastinal sampling though help in confirming granulomatous pathology, distinction between these entities and treatment decisions still depend upon correlating cytologic, microbiologic, clinical and radiological data in a large number of cases in tubercular endemic regions. (Source: CytoJournal)
Source: CytoJournal - September 26, 2013 Category: Pathology Authors: Gagandeep Kaur Amit Dhamija Jolsana Augustine Pooja Bakshi Kusum Verma Source Type: research
Macrofollicular variant of papillary carcinoma, a potential diagnostic pitfall: A report of two cases including a review of literature
We report the cytologic findings of two surgically confirmed MFVPTC. The first case showed abundant thin colloid with moderate amount of follicular cells arranged in a honeycombed and syncytial pattern. Some but not all the follicular cells showed enlarged round to ovoid nuclei, overlapping nuclei, few nuclear grooves and rare intranuclear inclusions. The second case showed abundant thin colloid and predominantly benign appearing follicular cells with few overlapping nuclei, enlarged round to ovoid nuclei and rare nuclear grooves. No intranuclear inclusions were identified. A review of the literature was done and the cytol...
Source: CytoJournal - August 30, 2013 Category: Pathology Authors: Maria Luisa C Policarpio-NicolasDeepika Sirohi Source Type: research
Cytomorphology of unusual primary tumors in the Pap test
Walid E Khalbuss, Liron Pantanowitz, Sara E MonacoCytoJournal 2013 10(1):17-17Rare entities in the Pap test, which include neoplastic and non-neoplastic conditions, pose challenges due to the infrequent occurrence of many of these entities in the daily practice of cytology. Furthermore, these conditions give rise to important diagnostic pitfalls to be aware of in the Pap test. For example, cases with adenoma malignum (AM) have been called benign. Recognition of these conditions can help correctly interpret Pap tests as abnormal and thereby ensure that patients get appropriately diagnosed. In this paper, we illustrate and d...
Source: CytoJournal - August 30, 2013 Category: Pathology Authors: Walid E KhalbussLiron PantanowitzSara E Monaco Source Type: research
Announcement of first time Cytojournal impact factor for 2012 coincides with Cytojournal decade celebration (2004-2013)
Vinod B Shidham, Richard M DeMayCytoJournal 2013 10(1):18-18 (Source: CytoJournal)
Source: CytoJournal - August 30, 2013 Category: Pathology Authors: Vinod B ShidhamRichard M DeMay Source Type: research
High risk human papillomavirus type 16 and 18 infection in the cervical lesions of women with epithelial cell abnormality in Pap smear: A cytohistomorphologic association in Bangladeshi women
Conclusion: The findings of this study will contribute to HPV 16/18 knowledge in Bangladesh that will be useful in assessing the success of current vaccines with limited type spectra and augmenting cervical cancer screening strategies. (Source: CytoJournal)
Source: CytoJournal - July 16, 2013 Category: Pathology Authors: Urmila BanikM Shahab Uddin AhamadPradip BhattacharjeeArun Kumar AdhikaryZillur Rahman Source Type: research
Indications for renal fine needle aspiration biopsy in the era of modern imaging modalities
Conclusions: Currently, renal fine needle aspiration remains a useful diagnostic tool in selected clinico-radiologic scenarios. (Source: CytoJournal)
Source: CytoJournal - July 16, 2013 Category: Pathology Authors: Ema A DragoescuLina Liu Source Type: research
