A Simple, Universal, and Cost-Efficient Digital PCR Method for the Targeted Analysis of Copy Number Variations [Molecular Diagnostics and Genetics]
CONCLUSIONS:
The universal dPCR CNV assay is simple, robust, and cost-efficient because it combines a straightforward design allowed by universal probes and end point PCR, the advantages of a relative quantification of the target to the reference within the same reaction, and the high flexibility of the LNA hydrolysis probes. This method should be a useful tool for genomic medicine, which requires simple methods for the interpretation and segregation analysis of genomic variations. (Source: Clinical Chemistry)
Source: Clinical Chemistry - September 2, 2019 Category: Chemistry Authors: Cassinari, K., Quenez, O., Joly-Helas, G., Beaussire, L., Le Meur, N., Castelain, M., Goldenberg, A., Guerrot, A.-M., Brehin, A.-C., Deleuze, J.-F., Boland, A., Rovelet-Lecrux, A., Campion, D., Saugier-Veber, P., Gruchy, N., Frebourg, T., Nicolas, G., Sar Tags: Molecular Diagnostics and Genetics Source Type: research
Islet Autoantibody Standardization Program 2018 Workshop: Interlaboratory Comparison of Glutamic Acid Decarboxylase Autoantibody Assay Performance [Clinical Immunology]
CONCLUSIONS:
Several novel assay formats submitted to this study showed heterogeneous performance. In 2018, the majority of the best performing GADA immunoassays consisted of novel or established nonradioactive tests that proved on a par or superior to the radiobinding assay, the previous gold standard assay format for GADA measurement. (Source: Clinical Chemistry)
Source: Clinical Chemistry - September 2, 2019 Category: Chemistry Authors: Lampasona, V., Pittman, D. L., Williams, A. J., Achenbach, P., Schlosser, M., Akolkar, B., Winter, W. E., Participating Laboratories Tags: Clinical Immunology Source Type: research
Multicenter Evaluation of Circulating Plasma MicroRNA Extraction Technologies for the Development of Clinically Feasible Reverse Transcription Quantitative PCR and Next-Generation Sequencing Analytical Work Flows [Cancer Diagnostics]
CONCLUSIONS:
The choice of plasma miRNA extraction methods affects the selection of potential miRNA marker candidates and mechanistic interpretation of results, which should be done with caution, particularly across studies using different protocols. (Source: Clinical Chemistry)
Source: Clinical Chemistry - September 2, 2019 Category: Chemistry Authors: Kloten, V., Neumann, M. H. D., Di Pasquale, F., Sprenger-Haussels, M., Shaffer, J. M., Schlumpberger, M., Herdean, A., Betsou, F., Ammerlaan, W., af Hallstrom, T., Serkkola, E., Forsman, T., Lianidou, E., Sjoback, R., Kubista, M., Bender, S., Lampignano, Tags: Cancer Diagnostics (since 2002) Source Type: research
Once-Per-Visit Alerts: A Means to Study Alert Compliance and Reduce Repeat Laboratory Testing [Evidence-Based Medicine and Test Utilization]
CONCLUSIONS:
A diverse set of provider and test characteristics influences compliance with once-per-visit laboratory alerts. Future alerts should incorporate these characteristics into alert design to minimize alert overrides. (Source: Clinical Chemistry)
Source: Clinical Chemistry - September 2, 2019 Category: Chemistry Authors: Szymanski, J. J., Qavi, A. J., Laux, K., Jackups, R. Tags: Evidence-Based Medicine and Test Utilization Source Type: research
ProBNP That Is Not Glycosylated at Threonine 71 Is Decreased with Obesity in Patients with Heart Failure [Current Issues in Laboratory Medicine]
CONCLUSIONS:
Increased BMI is associated with decreased concentrations of proBNP not glycosylated at T71. Decreased proBNP substrate amenable to processing could partially explain the lower NT-proBNP and BNP concentrations observed in obese individuals, including those presenting with HF. (Source: Clinical Chemistry)
Source: Clinical Chemistry - September 2, 2019 Category: Chemistry Authors: Lewis, L. K., Raudsepp, S. D., Prickett, T. C. R., Yandle, T. G., Doughty, R. N., Frampton, C. M., Pemberton, C. J., Richards, A. M. Tags: Current Issues in Laboratory Medicine Source Type: research
Direct Versus Calculated LDL Cholesterol and C-Reactive Protein in Cardiovascular Disease Risk Assessment in the Framingham Offspring Study [Lipids, Lipoproteins, and Cardiovascular Risk Factors]
CONCLUSIONS:
Our data indicate that the Denka Seiken direct LDL-C and Dade Behring hsCRP measurements add significant, but modest, information about CVD risk, compared to standard risk factors and/or calculated LDL-C. (Source: Clinical Chemistry)
Source: Clinical Chemistry - September 2, 2019 Category: Chemistry Authors: Ikezaki, H., Fisher, V. A., Lim, E., Ai, M., Liu, C.-T., Adrienne Cupples, L., Nakajima, K., Asztalos, B. F., Furusyo, N., Schaefer, E. J. Tags: Lipids, Lipoproteins, and Cardiovascular Risk Factors Source Type: research
Emergence of MicroRNAs as Key Players in Cancer Cell Metabolism [Review]
BACKGROUND:
Metabolic reprogramming is a hallmark of cancer. MicroRNAs (miRNAs) have been found to regulate cancer metabolism by regulating genes involved in metabolic pathways. Understanding this layer of complexity could lead to the development of novel therapeutic approaches.
CONTENT:
miRNAs are noncoding RNAs that have been implicated as master regulators of gene expression. Studies have revealed the role of miRNAs in the metabolic reprogramming of tumor cells, with several miRNAs both positively and negatively regulating multiple metabolic genes. The tricarboxylic acid (TCA) cycle, aerobic glycolysis, de novo fatty a...
Source: Clinical Chemistry - September 2, 2019 Category: Chemistry Authors: Subramaniam, S., Jeet, V., Clements, J. A., Gunter, J. H., Batra, J. Tags: Endocrinology and Metabolism Review Source Type: research
Commentary [Clinical Case Study]
(Source: Clinical Chemistry)
Source: Clinical Chemistry - September 2, 2019 Category: Chemistry Authors: Bossuyt, X., Ancheva, L. Tags: Clinical Case Study Source Type: research
A Rapidly Deteriorating Patient with Gross Increase in Serum Free Light Chains [Clinical Case Study]
(Source: Clinical Chemistry)
Source: Clinical Chemistry - September 2, 2019 Category: Chemistry Authors: Fung, A. W. S., Kohlhagen, M. C., DeMarco, M. L., Mills, J. R. Tags: Clinical Immunology, Clinical Case Studies Clinical Case Study Source Type: research
Role of BNP vs NT-proBNP Testing in the Age of New Drug Therapies: Sacubitril-Valsartan [Q&A]
(Source: Clinical Chemistry)
Source: Clinical Chemistry - September 2, 2019 Category: Chemistry Authors: Apple, F. S., Daniels, L. B., Januzzi, J., Omland, T., Ordonez-Llanos, J., Jaffe, A. Tags: Proteomics and Protein Markers Q & amp;A Source Type: research
Can Sweat Sensors Detect Common Diseases? A Simple Sweat Patch May Soon Achieve It [Perspective]
(Source: Clinical Chemistry)
Source: Clinical Chemistry - September 2, 2019 Category: Chemistry Authors: Christodouleas, D. C. Tags: Point-of-Care Testing, Perspective Source Type: research
A View on the Interrelationship between Obesity and Natriuretic Peptide Measurements: Can Dysregulation in pro-B-type Natriuretic Peptide Glycosylation Explain Decreased B-type Natriuretic Peptide Concentrations in Obese Heart Failure Patients? [Editorials]
(Source: Clinical Chemistry)
Source: Clinical Chemistry - September 2, 2019 Category: Chemistry Authors: Semenov, A. G., Katrukha, A. G. Tags: Proteomics and Protein Markers Editorials Source Type: research
LDL Cholesterol: What Is the Best Way to Measure It? [Editorials]
(Source: Clinical Chemistry)
Source: Clinical Chemistry - September 2, 2019 Category: Chemistry Authors: Wolska, A., Remaley, A. T. Tags: Lipids, Lipoproteins, and Cardiovascular Risk Factors Editorials Source Type: research
Baltic Coast [Unveiling the Right Side]
(Source: Clinical Chemistry)
Source: Clinical Chemistry - July 28, 2019 Category: Chemistry Authors: Dominiczak, M. H. Tags: Unveiling the Right Side Source Type: research
