A Simple, Universal, and Cost-Efficient Digital PCR Method for the Targeted Analysis of Copy Number Variations [Molecular Diagnostics and Genetics]

CONCLUSIONS:
The universal dPCR CNV assay is simple, robust, and cost-efficient because it combines a straightforward design allowed by universal probes and end point PCR, the advantages of a relative quantification of the target to the reference within the same reaction, and the high flexibility of the LNA hydrolysis probes. This method should be a useful tool for genomic medicine, which requires simple methods for the interpretation and segregation analysis of genomic variations.

Source: Clinical Chemistry - September 2, 2019 Category: Chemistry Authors: Cassinari, K., Quenez, O., Joly-Helas, G., Beaussire, L., Le Meur, N., Castelain, M., Goldenberg, A., Guerrot, A.-M., Brehin, A.-C., Deleuze, J.-F., Boland, A., Rovelet-Lecrux, A., Campion, D., Saugier-Veber, P., Gruchy, N., Frebourg, T., Nicolas, G., Sar Tags: Molecular Diagnostics and Genetics Source Type: research