Multicenter Evaluation of Circulating Plasma MicroRNA Extraction Technologies for the Development of Clinically Feasible Reverse Transcription Quantitative PCR and Next-Generation Sequencing Analytical Work Flows [Cancer Diagnostics]

CONCLUSIONS:
The choice of plasma miRNA extraction methods affects the selection of potential miRNA marker candidates and mechanistic interpretation of results, which should be done with caution, particularly across studies using different protocols.

Source: Clinical Chemistry - September 2, 2019 Category: Chemistry Authors: Kloten, V., Neumann, M. H. D., Di Pasquale, F., Sprenger-Haussels, M., Shaffer, J. M., Schlumpberger, M., Herdean, A., Betsou, F., Ammerlaan, W., af Hallstrom, T., Serkkola, E., Forsman, T., Lianidou, E., Sjoback, R., Kubista, M., Bender, S., Lampignano, Tags: Cancer Diagnostics (since 2002) Source Type: research