---
Biopolymers, Ahead of Print. (Source: Biopolymers)
Source: Biopolymers - October 25, 2017 Category: Biochemistry Source Type: research
Exploring the influence of hyperthermophilic protein Ssh10b on the stability and conformation of RNA by molecular dynamics simulation
This study increases our understanding of the dynamics and interaction mechanism of hyperthermophilic proteins and RNA at the atomic level, and offers a model for studying the structural biology of hyperthermophilic proteins and RNA. (Source: Biopolymers)
Source: Biopolymers - October 25, 2017 Category: Biochemistry Authors: Xue Zhang, Qing ‐Chuan Zheng Tags: ORIGINAL ARTICLE Source Type: research
Polypeptoid polymers: Synthesis, characterization, and properties
Abstract
Polypeptoids, a class of peptidomimetic polymers, have emerged at the forefront of macromolecular and supramolecular science and engineering as the technological relevance of these polymers continues to be demonstrated. The chemical and structural diversity of polypeptoids have enabled access to and adjustment of a variety of physicochemical and biological properties (eg, solubility, charge characteristics, chain conformation, HLB, thermal processability, degradability, cytotoxicity and immunogenicity). These attributes have made this synthetic polymer platform a potential candidate for various biomedical and biot...
Source: Biopolymers - October 25, 2017 Category: Biochemistry Authors: Brandon A. Chan, Sunting Xuan, Ang Li, Jessica M. Simpson, Garrett L. Sternhagen, Tianyi Yu, Omead A. Darvish, Naisheng Jiang, Donghui Zhang Tags: REVIEW Source Type: research
Doxorubicin hinders DNA condensation promoted by the protein bovine serum albumin (BSA)
Abstract
In this work, we have studied the interaction between the anticancer drug doxorubicin (doxo) and condensed DNA, using optical tweezers. To perform this task, we use the protein bovine serum albumin (BSA) in the working buffer to mimic two key conditions present in the real intracellular environment: the condensed state of the DNA and the abundant presence of charged macromolecules in the surrounding medium. In particular, we have found that, when doxo is previously intercalated in disperse DNA, the drug hinders the DNA condensation process upon the addition of BSA in the buffer. On the other hand, when bare DNA is...
Source: Biopolymers - October 23, 2017 Category: Biochemistry Authors: C. H. M. Lima, H. M. C. de Paula, L. H. M. da Silva, M. S. Rocha Tags: ORIGINAL ARTICLE Source Type: research
Issue Information
(Source: Biopolymers)
Source: Biopolymers - October 3, 2017 Category: Biochemistry Tags: ISSUE INFORMATION Source Type: research
Cover Image, Volume 107, Issue 11
The mitotic spindle is a complex structure formed by microtubules that assembles during cell division in order to distribute genetic material. Mitotic spindle assembly depends on TACC3, a centrosomal adaptor protein, interacting with the microtubule polymerase chTOG/XMAP215. Bruix et al. use a model based on two small peptides derived from sequences spanning two regions of the proteins XTACC3 (red and orange helices) and XMAP215 (blue helices) to study the system. The results of NMR and CD experiments show that all peptides tend to adopt helical conformations and that the simultaneous presence of all helices is essential f...
Source: Biopolymers - October 3, 2017 Category: Biochemistry Tags: COVER IMAGE Source Type: research
Antimicrobial activity of lysozyme isoforms: Key molecular features
Abstract
Increasing bacterial resistance towards antibiotics has stimulated research for novel antimicrobials. Proteins acting on bacterial membranes could be a solution. Lysozyme has been proven active against E. coli by disruption of both outer and cytoplasmic membranes, with dry‐heating increasing lysozyme activity. Dry‐heated lysozyme (DH‐L) is a mixture of isoforms (isoaspartyl, native‐like and succinimide lysozymes), giving rise to two questions: what effects does each form have, and which physicochemical properties are critical as regards the antibacterial activity? These issues were investigated by fraction...
Source: Biopolymers - September 25, 2017 Category: Biochemistry Authors: Melanie Derde, V éronique Vié, Astrid Walrant, Sandrine Sagan, Valérie Lechevalier, Catherine Guérin‐Dubiard, Stéphane Pezennec, Marie‐Françoise Cochet, Gilles Paboeuf, Maryvonne Pasco, Florence Baron, Michel Gautier, Sophie Jan, Françoise Nau Tags: ORIGINAL ARTICLE Source Type: research
Highly efficiency antibacterial diblock copolypeptides based on lysine and phenylalanine
Abstract
A series of amphiphilic diblock copolypeptides (K30‐b‐F15, K30‐b‐F30, and K30‐b‐F45) were synthesized via N‐carboxy‐α‐amino‐anhydride ring‐opening polymerization. The copolypeptides had excellent antibacterial efficacy to both Gram positive (S. aureus) and Gram negative (E. coli) bacteria. The minimum inhibitory concentrations (MICs) against E. coli and S. aureus are 8 μg mL−1 and 2 μg mL−1, respectively, lower than most natural and artificial antimicrobial peptides (AMPs). The morphological changes of the bacteria treated with diblock copolypeptides were investigated by transmissi...
Source: Biopolymers - September 19, 2017 Category: Biochemistry Authors: Xiaokai Su, Xinyu Zhou, Zhengzhong Tan, Chuncai Zhou Tags: ORIGINAL ARTICLE Source Type: research
Thermal melt circular dichroism spectroscopic studies for identifying stabilising amphipathic molecules for the voltage ‐gated sodium channel NavMs
Biopolymers, EarlyView. (Source: Biopolymers)
Source: Biopolymers - September 19, 2017 Category: Biochemistry Authors: Sam M.Ireland
,
AltinSula
,
B.A.Wallace Source Type: research
---
Biopolymers, Ahead of Print. (Source: Biopolymers)
Source: Biopolymers - September 19, 2017 Category: Biochemistry Source Type: research
Highly efficient antibacterial diblock copolypeptides based on lysine and phenylalanine
Abstract
A series of amphiphilic diblock copolypeptides (K30‐b‐F15, K30‐b‐F30, and K30‐b‐F45) were synthesized via N‐carboxy‐α‐amino‐anhydride ring‐opening polymerization. The copolypeptides had excellent antibacterial efficacy to both Gram positive (S. aureus) and Gram negative (E. coli) bacteria. The minimum inhibitory concentrations (MICs) against E. coli and S. aureus are 8 μg mL−1 and 2 μg mL−1, respectively, lower than most natural and artificial antimicrobial peptides (AMPs). The morphological changes of the bacteria treated with diblock copolypeptides were investigated by transmissi...
Source: Biopolymers - September 19, 2017 Category: Biochemistry Authors: Xiaokai Su, Xinyu Zhou, Zhengzhong Tan, Chuncai Zhou Tags: ORIGINAL ARTICLE Source Type: research
Thermal melt circular dichroism spectroscopic studies for identifying stabilising amphipathic molecules for the voltage ‐gated sodium channel NavMs
In this study we used circular dichroism spectroscopy to compare the secondary structures and thermal stabilities of the NavMs voltage‐gated sodium channel in different amphipols and detergents as a means of identifying amphipathic environments that maximally maintain the protein structure whilst providing a stabilising environment. These types of characterisations also have potential as means of screening for sample types that may be more suitable for crystallisation and/or cryo‐electron microscopy structure determinations. (Source: Biopolymers)
Source: Biopolymers - September 19, 2017 Category: Biochemistry Authors: Sam M. Ireland, Altin Sula, B.A. Wallace Tags: ORIGINAL ARTICLE Source Type: research
Structural insight into the XTACC3/XMAP215 interaction from CD and NMR studies on model peptides
Abstract
TACC3 is a centrosomal adaptor protein that plays important roles during mitotic spindle assembly. It interacts with chTOG/XMAP215, which catalyzes the addition of tubulin dimers during microtubule growth. A 3D coiled‐coil model for this interaction is available but the structural details are not well described. To characterize this interaction at atomic resolution, we have designed a simplified version of the system based on small peptides. Four different peptides have been studied by circular dichroism and nuclear magnetic resonance both singly and in all possible combinations; namely, five peptide pairs and t...
Source: Biopolymers - September 18, 2017 Category: Biochemistry Authors: Ang élica Partida‐Hanon, Miguel A. Treviño, Miguel Mompeán, Ma Ángeles Jiménez, Marta Bruix Tags: ORIGINAL ARTICLE Source Type: research
