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Biopolymers, Ahead of Print. (Source: Biopolymers)
Source: Biopolymers - January 11, 2018 Category: Biochemistry Source Type: research
A long ‐lived Aβ oligomer resistant to fibrillization
Biopolymers, EarlyView. (Source: Biopolymers)
Source: Biopolymers - January 10, 2018 Category: Biochemistry Authors: MimiNick
,
YibingWu
,
Nathan W.Schmidt
,
Stanley B.Prusiner
,
JanSt öhr
,
William F.DeGrado Source Type: research
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Biopolymers, Ahead of Print. (Source: Biopolymers)
Source: Biopolymers - January 10, 2018 Category: Biochemistry Source Type: research
Oxidation catalysis by iron and manganese porphyrins within enzyme ‐like cages
Abstract
Inspired by natural heme‐proteins, scientists have attempted for decades to design efficient and selective metalloporphyrin‐based oxidation catalysts. Starting from the pioneering work on small molecule mimics in the late 1970s, we have assisted to a tremendous progress in designing cages of different nature and complexity, able to accommodate metalloporphyrins. With the intent of tuning and controlling their reactivity, more and more sophisticated and diverse environments are continuously exploited. In this review, we will survey the current state of art in oxidation catalysis using iron‐ and manganese‐po...
Source: Biopolymers - January 1, 2018 Category: Biochemistry Authors: Marco Chino, Linda Leone, Gerardo Zambrano, Fabio Pirro, Daniele D'Alonzo, Vincenzo Firpo, Diaa Aref, Liliana Lista, Ornella Maglio, Flavia Nastri, Angela Lombardi Tags: REVIEW Source Type: research
“Cooperative collapse” of the denatured state revealed through Clausius‐Clapeyron analysis of protein denaturation phase diagrams
Abstract
Protein phase diagrams have a unique potential to identify the presence of additional thermodynamic states even when non‐2‐state character is not readily apparent from the experimental observables used to follow protein unfolding transitions. Two‐state analysis of the von Willebrand factor A3 domain has previously revealed a discrepancy in the calorimetric enthalpy obtained from thermal unfolding transitions as compared with Gibbs‐Helmholtz analysis of free energies obtained from the Linear Extrapolation Method (Tischer and Auton, Prot Sci 2013; 22(9):1147‐60). We resolve this thermodynamic conundrum usi...
Source: Biopolymers - January 1, 2018 Category: Biochemistry Authors: Alexander Tischer, Venkata R. Machha, J örg Rösgen, Matthew Auton Tags: ORIGINAL ARTICLE Source Type: research
Counteraction ability of TMAO toward different denaturing agents
Abstract
Differential scanning calorimetry measurements performed on RNase A in aqueous binary solutions containing different concentrations of urea, tetramethylurea, guanidinium chloride, and guanidinium thiocyanate, and in aqueous ternary solutions, containing the same denaturants plus 1 M trimethylamine N‐oxide, TMAO, demonstrate that the latter has a general counteracting ability at pH 7.0, but not at pH 4.0. Experimental data rule out the idea that counteraction originates from direct interactions between TMAO molecules and denaturing agents. A rationalization is provided on the basis of a theoretical approach groun...
Source: Biopolymers - January 1, 2018 Category: Biochemistry Authors: Marilisa Vigorita, Serena Cozzolino, Rosario Oliva, Giuseppe Graziano, Pompea Del Vecchio Tags: ORIGINAL ARTICLE Source Type: research
The effect of interleukin ‐8 truncations on its interactions with glycosaminoglycans
In this study, we applied experimental and computational techniques to investigate the binding of wild type and truncated IL‐8 variants to natural and chemically modified GAGs to gain further insight into the IL‐8/GAG interaction. Circular dichroism spectroscopy of IL‐8 variants did not reveal major structural changes upon GAG binding. Heparin affinity chromatography clearly demonstrates that gradual truncation of the C‐terminal helix leads to decreasing affinities. Similarly, surface plasmon resonance indicates participation of both IL‐8 termini in GAG binding, which strength is dependent on GAG sulfation degree...
Source: Biopolymers - January 1, 2018 Category: Biochemistry Authors: Karoline Nordsieck, Lars Baumann, Vera Hintze, M. Teresa Pisabarro, Matthias Schnabelrauch, Annette G. Beck ‐Sickinger, Sergey A. Samsonov Tags: ORIGINAL ARTICLE Source Type: research
Alterations in local stability and dynamics of A4V SOD1 in the presence of trifluoroethanol
Abstract
Alterations in the local dynamics of Cu/Zn Superoxide dismutase (SOD1) due to mutations affect the protein folding, stability, and function leading to misfolding and aggregation seen in amyotrophic lateral sclerosis (ALS). Here, we study the structure and dynamics of the most devastating ALS mutation, A4V SOD1 in aqueous trifluoroethanol (TFE) through experiments and simulation. Far‐UV circular dichroism (CD) studies shows that TFE at intermediate concentrations (∼15% ‐ 30%) induce partially unfolded β‐sheet‐rich extended conformations in A4V SOD1 which subsequently aggregates. Molecular dynamics (MD) s...
Source: Biopolymers - January 1, 2018 Category: Biochemistry Authors: Vijay Kumar, Amresh Prakash, Andrew M. Lynn Tags: ORIGINAL ARTICLE Source Type: research
Jack Aviv and brains of children
Biopolymers, EarlyView. (Source: Biopolymers)
Source: Biopolymers - December 4, 2017 Category: Biochemistry Authors: Angelo A.Lamola Source Type: research
