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Biopolymers, Ahead of Print. (Source: Biopolymers)
Source: Biopolymers - December 4, 2017 Category: Biochemistry Source Type: research
Impact of bistrand abasic sites and proximate orientation on DNA global structure and duplex energetics
This study characterizes the structural and energy profiles of DNA duplexes harboring synthetic abasic sites (tetrahydrofuran, F) as models of clustered bistrand abasic (AP) lesions. The standard tridecameric dGCGTACCCATGCG·dCGCATGGGTACGC duplex is employed to investigate the energetic impact of single and bistrand AP sites by strategically replacing one or two bases within the central CCC/GGG triplet. Our combined analysis of temperature‐dependent UV and circular dichroism (CD) profiles reveals that the proximity and relative orientation of AP sites within bistrand‐damaged duplexes imparts a significant thermodynamic...
Source: Biopolymers - December 1, 2017 Category: Biochemistry Authors: Concei ção A. Minetti, Jeffrey Y. Sun, Daniel P. Jacobs, Inkoo Kang, David P. Remeta, Kenneth J. Breslauer Tags: ORIGINAL ARTICLE Source Type: research
A long ‐lived aβ oligomer resistant to fibrillization
Abstract
The hydrophobic Aβ peptide is highly aggregation prone; it first forms soluble oligomers, which then convert into the amyloid fibrils found in the cerebral plaques of Alzheimer's disease. It is generally understood that as the peptide concentration of Aβ increases, the fibrillization process is accelerated, but we examine the limits on this phenomenon. We found that once a threshold concentration of Aβ is exceeded, a stable oligomer is formed at the expense of fibril formation. The suppression of fibril formation was observed by amyloid‐binding dye Thioflavin T and solution nuclear magnetic resonance (NMR). S...
Source: Biopolymers - December 1, 2017 Category: Biochemistry Authors: Mimi Nick, Yibing Wu, Nathan W. Schmidt, Stanley B. Prusiner, Jan St öhr, William F. DeGrado Tags: ORIGINAL ARTICLE Source Type: research
Jack Aviv and brains of children
This article sketches Jack Aviv's contribution to the development and application of hematofluorometry. (Source: Biopolymers)
Source: Biopolymers - December 1, 2017 Category: Biochemistry Authors: Angelo A. Lamola Tags: REVIEW Source Type: research
Enzymatic degradation of hyaluronan hydrogels with different capacity for in situ bio ‐mineralization
Abstract
In situ cross‐linked hyaluronan (HA) hydrogels with different capacities for biomineralization were prepared and their enzymatic degradation was monitored. Covalent incorporation of bisphosphonates (BPs) into HA hydrogel results in the increased stiffness of the hydrogel in comparison with the unmodified HA hydrogel of the same cross‐linking density. The rate of enzymatic degradation of HABP hydrogel was significantly lower than the rate of degradation of control HA hydrogel in vitro. This effect is observed only in the presence of calcium ions that strongly bind to the matrix‐anchored BP groups and promote ...
Source: Biopolymers - November 28, 2017 Category: Biochemistry Authors: Liyang Shi, Yu Zhang, Dmitri Ossipov Tags: ORIGINAL ARTICLE Source Type: research
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Biopolymers,Volume 109, Issue 2, February 2018. (Source: Biopolymers)
Source: Biopolymers - November 27, 2017 Category: Biochemistry Source Type: research
Probing the activity of a recombinant Zn2+ ‐transporting P‐type ATPase
Abstract
P‐type ATPase proteins maintain cellular homeostasis and uphold critical concentration gradients by ATP‐driven ion transport across biological membranes. Characterization of single‐cycle dynamics by time‐resolved X‐ray scattering techniques in solution could resolve structural intermediates not amendable to for example crystallization or cryo‐electron microscopy sample preparation. To pave way for such time‐resolved experiments, we used biochemical activity measurements, Attenuated Total Reflectance (ATR) and time‐dependent Fourier‐Transform Infra‐Red (FTIR) spectroscopy to identify optimal con...
Source: Biopolymers - November 23, 2017 Category: Biochemistry Authors: H. Ravishankar, A. Barth, M. Andersson Tags: ORIGINAL ARTICLE Source Type: research
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Biopolymers,Volume 109, Issue 2, February 2018. (Source: Biopolymers)
Source: Biopolymers - November 23, 2017 Category: Biochemistry Source Type: research
Design and development of low cost polyurethane biopolymer based on castor oil and glycerol for biomedical applications
This study opens an avenue for using low cost bio‐polyurethane based on castor oil and glycerol for biomedical applications. (Source: Biopolymers)
Source: Biopolymers - November 21, 2017 Category: Biochemistry Authors: A. C. W. Tan, B. J. Polo ‐Cambronell, E. Provaggi, C. Ardila‐Suárez, G. E. Ramirez‐Caballero, V. G. Baldovino‐Medrano, D. M. Kalaskar Tags: ORIGINAL ARTICLE Source Type: research
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Biopolymers,Volume 109, Issue 2, February 2018. (Source: Biopolymers)
Source: Biopolymers - November 21, 2017 Category: Biochemistry Source Type: research
The burst ‐phase folding intermediate of ribonuclease H changes conformation over evolutionary history
Abstract
The amino acid sequence encodes the energy landscape of a protein. Therefore, we expect evolutionary mutations to change features of the protein energy landscape, including the conformations adopted by a polypeptide as it folds to its native state. Ribonucleases H (RNase H) from Escherichia coli and Thermus thermophilus both fold via a partially folded intermediate in which the core region of the protein (helices A‐D and strands 4‐5) is structured. Strand 1, however, uniquely contributes to the T. thermophilus RNase H folding intermediate (Icore+1), but not the E. coli RNase H intermediate (Icore) (Rosen &...
Source: Biopolymers - November 20, 2017 Category: Biochemistry Authors: Shion A. Lim, Susan Marqusee Tags: ORIGINAL ARTICLE Source Type: research
The burst ‐phase folding intermediate of ribonuclease H changes conformation over evolutionary history
Biopolymers, EarlyView. (Source: Biopolymers)
Source: Biopolymers - November 20, 2017 Category: Biochemistry Authors: Shion A.Lim
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SusanMarqusee Source Type: research
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Biopolymers, Ahead of Print. (Source: Biopolymers)
Source: Biopolymers - November 20, 2017 Category: Biochemistry Source Type: research
