Mechanisms of GTP hydrolysis and conformational transitions in the dynamin superfamily
(Source: Biopolymers)
Source: Biopolymers - November 17, 2017 Category: Biochemistry Authors: Oliver Daumke, Gerrit J. K. Praefcke Tags: CORRIGENDUM Source Type: research
---
Biopolymers,Volume 109, Issue 2, February 2018. (Source: Biopolymers)
Source: Biopolymers - November 17, 2017 Category: Biochemistry Source Type: research
Β‐cyclodextrin encapsulated polyphenols as effective antioxidants
Abstract
Formation of dityrosine (DT) cross‐linkages in proteins is one of the most widely used markers of oxidative stress. Ribonuclease A (RNase A) has 6 Tyr residues and shows a characteristic DT fluorescence peak upon oxidation in addition to major changes in its secondary structure. DT formation can be prevented by using polyphenols (GA, ECG, and EGCG) which are known to have strong antioxidant activity. However, it has been observed that ECG and EGCG initiate protein oligomerization due to protein‐polyphenol cross‐linkages. To prevent the formation of such cross‐linkages we have used β‐cyclodextrin (β‐C...
Source: Biopolymers - November 15, 2017 Category: Biochemistry Authors: Pritam Roy, Amit Kumar Dinda, Susmitnarayan Chaudhury, Swagata Dasgupta Tags: ORIGINAL ARTICLE Source Type: research
---
Biopolymers,Volume 109, Issue 2, February 2018. (Source: Biopolymers)
Source: Biopolymers - November 15, 2017 Category: Biochemistry Source Type: research
β‐cyclodextrin encapsulated polyphenols as effective antioxidants
Abstract
Formation of dityrosine (DT) cross‐linkages in proteins is one of the most widely used markers of oxidative stress. Ribonuclease A (RNase A) has 6 Tyr residues and shows a characteristic DT fluorescence peak upon oxidation in addition to major changes in its secondary structure. DT formation can be prevented by using polyphenols (GA, ECG, and EGCG) which are known to have strong antioxidant activity. However, it has been observed that ECG and EGCG initiate protein oligomerization due to protein‐polyphenol cross‐linkages. To prevent the formation of such cross‐linkages we have used β‐cyclodextrin (β‐C...
Source: Biopolymers - November 15, 2017 Category: Biochemistry Authors: Pritam Roy, Amit Kumar Dinda, Susmitnarayan Chaudhury, Swagata Dasgupta Tags: ORIGINAL ARTICLE Source Type: research
Differentiation potential of human adipose stem cells bioprinted with hyaluronic acid/gelatin ‐based bioink through microextrusion and visible light‐initiated crosslinking
Abstract
Bioprinting has a great potential to fabricate three‐dimensional (3D) functional tissues and organs. In particular, the technique enables fabrication of 3D constructs containing stem cells while maintaining cell proliferation and differentiation abilities, which is believed to be promising in the fields of tissue engineering and regenerative medicine. We aimed to demonstrate the utility of the bioprinting technique to create hydrogel constructs consisting of hyaluronic acid (HA) and gelatin derivatives through irradiation by visible light to fabricate 3D constructs containing human adipose stem cells (hADSCs). T...
Source: Biopolymers - November 15, 2017 Category: Biochemistry Authors: Shinji Sakai, Hiromi Ohi, Tomoki Hotta, Hidenori Kamei, Masahito Taya Tags: ORIGINAL ARTICLE Source Type: research
Enhanced tissue adhesiveness of injectable gelatin hydrogels through dual catalytic activity of horseradish peroxidase
Abstract
Development of bioadhesives with tunable mechanical strength, high adhesiveness, biocompatibility, and injectability is greatly desirable in all surgeries to replace or complement the sutures and staples. Herein, the dual catalytic activity of horseradish peroxidase is exploited to in situ form the hydroxyphenyl propionic acid‐gelatin/thiolated gelatin (GH/GS) adhesive hydrogels including two alternative crosslinks (phenol‐phenol and disulfide bonds) with fast gelation (few seconds – several minutes) and improved physicochemical properties. Their elastic moduli increase from 6.7 to 10.3 kPa by adding GS poly...
Source: Biopolymers - November 6, 2017 Category: Biochemistry Authors: Thai Thanh Hoang Thi, Yunki Lee, Seung Bae Ryu, Dai Hai Nguyen, Ki Dong Park Tags: ORIGINAL ARTICLE Source Type: research
Issue Information
(Source: Biopolymers)
Source: Biopolymers - November 2, 2017 Category: Biochemistry Tags: ISSUE INFORMATION Source Type: research
Cover Image, Volume 107, Issue 12
DNA interactions with drugs are often examined in ordinary buffers where the DNA molecule is fully dispersed in solution – far from the state it is found in cells. Rocha et al. add bovine serum albumin (BSA) to mimic two conditions in the intracellular environment: the condensed state of DNA, and the abundance of charged macromolecules. When the anticancer drug doxorubicin is added to DNA already condensed by BSA, doxorubicin intercalates and unfolds the DNA condensates. However, if the drug is first intercalated in dispersed DNA, it hinders DNA condensation upon addition of BSA. The results may help our understanding th...
Source: Biopolymers - November 2, 2017 Category: Biochemistry Tags: COVER IMAGE Source Type: research
Probing the dynamics of restriction endonuclease NgoMIV ‐DNA interaction by single‐molecule FRET
Abstract
Many type II restriction endonucleases require two copies of their recognition sequence for optimal activity. Concomitant binding of two DNA sites by such an enzyme produces a DNA loop. Here we exploit single‐molecule Förster resonance energy transfer (smFRET) of surface‐immobilized DNA fragments to study the dynamics of DNA looping induced by tetrameric endonuclease NgoMIV. We have employed a DNA fragment with two NgoMIV recognition sites and a FRET dye pair such that upon protein‐induced DNA looping the dyes are brought to close proximity resulting in a FRET signal. The dynamics of DNA‐NgoMIV interactio...
Source: Biopolymers - October 27, 2017 Category: Biochemistry Authors: Marijonas Tutkus, Giedrius Sasnauskas, Danielis Rutkauskas Tags: ORIGINAL ARTICLE Source Type: research
The polypeptide biophysics of proline/alanine ‐rich sequences (PAS): Recombinant biopolymers with PEG‐like properties
Abstract
PAS polypeptides comprise long repetitive sequences of the small L‐amino acids proline, alanine and/or serine that were developed to expand the hydrodynamic volume of conjugated pharmaceuticals and prolong their plasma half‐life by retarding kidney filtration. Here, we have characterized the polymer properties both of the free polypeptides and in fusion with the biopharmaceutical IL‐1Ra. Data from size exclusion chromatography, dynamic light scattering, circular dichroism spectroscopy and quantification of hydrodynamic and polar properties demonstrate that the biosynthetic PAS polypeptides exhibit random coi...
Source: Biopolymers - October 27, 2017 Category: Biochemistry Authors: Joscha Breibeck, Arne Skerra Tags: ORIGINAL ARTICLE Source Type: research
Effect of heavy water on the conformational stability of globular proteins
Abstract
It is well established from the experimental point of view that the native state of globular proteins is more stable in heavy water than in water. No robust explanation, however, has been provided up to now. The application of the theoretical approach, originally devised to rationalize the general occurrence of cold denaturation, indicates that the magnitude of the solvent‐excluded volume effect is slightly smaller in heavy water than in water and cannot explain the observed protein stabilization. The latter has to be due to the strength of protein‐water van der Waals attractions which are weaker in heavy wate...
Source: Biopolymers - October 25, 2017 Category: Biochemistry Authors: Andrea Pica, Giuseppe Graziano Tags: ORIGINAL ARTICLE Source Type: research
Effect of heavy water on the conformational stability of globular proteins
Biopolymers, EarlyView. (Source: Biopolymers)
Source: Biopolymers - October 25, 2017 Category: Biochemistry Authors: AndreaPica
,
GiuseppeGraziano Source Type: research
