Thermal melt circular dichroism spectroscopic studies for identifying stabilising amphipathic molecules for the voltage ‐gated sodium channel NavMs

In this study we used circular dichroism spectroscopy to compare the secondary structures and thermal stabilities of the NavMs voltage‐gated sodium channel in different amphipols and detergents as a means of identifying amphipathic environments that maximally maintain the protein structure whilst providing a stabilising environment. These types of characterisations also have potential as means of screening for sample types that may be more suitable for crystallisation and/or cryo‐electron microscopy structure determinations.
Source: Biopolymers - Category: Biochemistry Authors: Tags: ORIGINAL ARTICLE Source Type: research