Characterization of Acr2, an H-NS-like protein encoded on A/C2-type plasmids
This study further characterizes Acr2 and suggests its role in modulating gene expression of multiple plasmid and chromosomal loci. (Source: Plasmid)
Source: Plasmid - August 2, 2016 Category: Biotechnology Source Type: research
pXO16 from Bacillus thuringiensis serovar israelensis: Almost 350 kb of terra incognita
Publication date: July 2015 Source:Plasmid, Volume 80 Author(s): Lionel Makart, Annika Gillis, Jacques Mahillon Bacillus thuringiensis strains usually harbor large sets of plasmids, some of which carrying the entomopathogenic δ-endotoxins. B. thuringiensis serovar israelensis, active on Dipteran larvae, carries the very large conjugative plasmid pXO16 (350 kb). pXO16 displays a macroscopic aggregation phenotype when liquid cultures of conjugative partners are mixed. Its conjugative apparatus is able of transferring itself and other non-conjugative and non-mobilizable plasmids in a fast and very efficient mann...
Source: Plasmid - July 20, 2016 Category: Biotechnology Source Type: research
Development of a new pCAMBIA binary vector using Gateway ® technology
Publication date: September 2015 Source:Plasmid, Volume 81 Author(s): Julie Leclercq, Toth Szabolcs, Florence Martin, Pascal Montoro pCAMBIA vectors have become popular for their easy handling, stability and the existence of a range of selection and reporter genes. However, these vectors have yet to integrate the Gateway® cloning system, which has enabled site-specific recombination without the need for restriction enzymes and ligases. This paper sets out to convert the pCambia2300 binary vector into a destination vector with the Gateway® cassette driven by the CaMV35S promoter. The destination vector, pCamwa...
Source: Plasmid - July 20, 2016 Category: Biotechnology Source Type: research
Graphene oxide –cationic polymer conjugates: Synthesis and application as gene delivery vectors
Publication date: March–May 2016 Source:Plasmid, Volumes 84–85 Author(s): Mohsen Teimouri, Azadeh Hashem Nia, Khalil Abnous, Hossein Eshghi, Mohammad Ramezani Nanomedicine as the interface between nanotechnology and medical sciences is a new area that has attracted the attention of vast groups of researchers. Carbon nanomaterials are common platform for synthesis of nanoparticles for biomedical applications due to their low cytotoxicity and feasible internalization into mammalian cell lines (Yang et al., 2007; Arora et al., 2014; Oh and Park, 2014). Synthesis of vectors based on various cationic polymers ...
Source: Plasmid - July 20, 2016 Category: Biotechnology Source Type: research
The N-terminus of IntDOT forms hydrophobic interactions during Holliday junction resolution
Publication date: Available online 12 July 2016 Source:Plasmid Author(s): Adam J. Kolakowski, Jeffrey F. Gardner DOT Integrase (IntDOT) is a member of the tyrosine recombinase family. It catalyzes the integration and excision reactions of an integrative and conjugative element (ICE) called CTnDOT. Like other tyrosine recombinases, the integration reaction proceeds by two sets of strand exchanges between the attDOT site on CTnDOT and an attB site in the host chromosome. The strand exchanges occur seven bases apart and define an overlap region. After the first strand exchanges a Holliday Junction (HJ) intermediate is...
Source: Plasmid - July 13, 2016 Category: Biotechnology Source Type: research
A set of mini-Mu transposons for versatile cloning of circular DNA and novel dual-transposon strategy for increased efficiency
We report here the construction of a platform for the purpose that contains ten mini-Mu transposons with five different replication origins, enabling a variety of research approaches for the discovery and study of circular DNA. We also demonstrate that the simultaneous use of two transposons, one with the origin of replication and the other with selectable marker, is beneficial as it improves the cloning efficiency by reducing the fraction of autointegration-derived plasmid clones. The constructed transposons now provide a set of new tools for the studies on DNA circles and widen the applicability of Mu transposition based...
Source: Plasmid - July 4, 2016 Category: Biotechnology Source Type: research
Biology of ICEBs1, an integrative and conjugative element in Bacillus subtilis
Publication date: Available online 2 July 2016 Source:Plasmid Author(s): Jennifer M. Auchtung, Naira Aleksanyan, Artemisa Bulku, Melanie B. Berkmen Horizontal gene transfer plays a profound role in bacterial evolution by propelling the rapid transfer of genes and gene cassettes. Integrative and conjugative elements (ICEs) are one important mechanism driving horizontal gene transfer. ICEs, also known as conjugative transposons, reside on the host chromosome but can excise to form a conjugative DNA circle that is capable of transfer to other cells. Analysis of the large number of completed bacterial genome sequen...
Source: Plasmid - July 2, 2016 Category: Biotechnology Source Type: research
Graphene oxide–cationic polymer conjugates: Synthesis and application as gene delivery vectors
Publication date: March–May 2016 Source:Plasmid, Volumes 84–85 Author(s): Mohsen Teimouri, Azadeh Hashem Nia, Khalil Abnous, Hossein Eshghi, Mohammad Ramezani Nanomedicine as the interface between nanotechnology and medical sciences is a new area that has attracted the attention of vast groups of researchers. Carbon nanomaterials are common platform for synthesis of nanoparticles for biomedical applications due to their low cytotoxicity and feasible internalization into mammalian cell lines (Yang et al., 2007; Arora et al., 2014; Oh and Park, 2014). Synthesis of vectors based on various cationic polymers ...
Source: Plasmid - June 15, 2016 Category: Biotechnology Source Type: research
Complete nucleotide sequence of pH11, an IncHI2 plasmid conferring multi-antibiotic resistance and multi-heavy metal resistance genes in a clinical Klebsiella pneumoniae isolate
Publication date: Available online 19 April 2016 Source:Plasmid Author(s): Yao Zhai, Zilong He, Yu Kang, Haiying Yu, Jianfeng Wang, Pengcheng Du, Zhao Zhang, Songnian Hu, Zhancheng Gao The complete 284,628bp sequence of pH11, an IncHI2 plasmid, was determined through single-molecule, real-time (SMRT) sequencing. Harbored by a clinical Klebsiella pneumoniae strain H11, and isolated in Beijing, this plasmid contains multiple antibiotic resistance genes, including catA2, aac(6′)-Ib, strB, strA, dfrA19, bla TEM-1, bla SHV-12, sul1, qacE delta 1, ereA, arr2, and aac3. The aac(6′)-Ib is carried by a cla...
Source: Plasmid - June 15, 2016 Category: Biotechnology Source Type: research
New high-cloning-efficiency vectors for complementation studies and recombinant protein overproduction in Escherichia coli and Salmonella enterica
Publication date: Available online 24 May 2016 Source:Plasmid Author(s): C.M. VanDrisse, J.C. Escalante-Semerena Galloway et al. recently described a method to alter vectors to include Type IIS restriction enzymes for high efficiency cloning. Utilizing this method, the multiple cloning sites of complementation and overexpression vectors commonly used in our laboratory were altered to contain recognition sequences of the Type IIS restriction enzyme, BspQI. Use of this enzyme increased the rate of cloning success to >97% efficiency. L(+)-Arabinose-inducible complementation vectors and overexpression vectors en...
Source: Plasmid - June 15, 2016 Category: Biotechnology Source Type: research
Proteolysis in plasmid DNA stable maintenance in bacterial cells
Publication date: Available online 29 May 2016 Source:Plasmid Author(s): Anna Karlowicz, Katarzyna Wegrzyn, Andrzej Dubiel, Malgorzata Ropelewska, Igor Konieczny Plasmids, as extrachromosomal genetic elements, need to work out strategies that promote independent replication and stable maintenance in host bacterial cells. Their maintenance depends on constant formation and dissociation of nucleoprotein complexes formed on plasmid DNA. Plasmid replication initiation proteins (Rep) form specific complexes on direct repeats (iterons) localized within the plasmid replication origin. Formation of these complexes al...
Source: Plasmid - June 15, 2016 Category: Biotechnology Source Type: research
Novel plasmid conferring kanamycin and tetracycline resistance in the Turkey-derived Campylobacter jejuni strain 11601MD
Publication date: Available online 4 June 2016 Source:Plasmid Author(s): M.D. Crespo, E. Altermann, J. Olson, W.G. Miller, K. Chandrashekhar, S. Kathariou In Campylobacter spp., resistance to the antimicrobials kanamycin and tetracycline is frequently associated with plasmid-borne genes. However, relatively few plasmids of Campylobacter jejuni have been fully characterized to date. A novel plasmid (p11601MD; 44,095nt) harboring tet(O) was identified in C. jejuni strain 11601MD, which was isolated from the jejunum of a turkey produced conventionally in North Carolina. Analysis of the p11601MD sequence reveal...
Source: Plasmid - June 15, 2016 Category: Biotechnology Source Type: research
pDGO100, a type 1 IncC plasmid from 1981 carrying ARI-A and a Tn1696-like transposon in a novel integrating element
Publication date: Available online 16 June 2016 Source:Plasmid Author(s): Christopher J. Harmer, Sally R. Partridge, Ruth M. Hall Most A/C plasmids sequenced to date were recovered in the last two decades. To gain insight into the evolution of this group, the IncC plasmid pDGO100, found in a multiply antibiotic-resistant Escherichia coli strain isolated in 1981, was sequenced. pDGO100 belongs to the type 1 lineage and carries an ARI-A antibiotic resistance island but not an ARI-B island. The A/C2 backbone of pDGO100 has a deletion in the rhs1 gene previously found in pRMH760 and differs by only six single base pa...
Source: Plasmid - June 15, 2016 Category: Biotechnology Source Type: research
Effect of antibiotics on extracellular protein level in Pseudomonas aeruginosa
Publication date: Available online 17 March 2016 Source:Plasmid Author(s): Eigo Takahashi, Jae Man Lee, Hiroaki Mon, Yuuka Chieda, Chisa Yasunaga-Aoki, Takahiro Kusakabe, Kazuhiro Iiyama Pseudomonas aeruginosa PAO1 organisms harbouring different plasmids were cultured in broths containing appropriate antibiotic(s). Extracellular proteins were more abundant in the presence of tetracycline or kanamycin than in the presence of other antibiotics. Zymography revealed that alkaline protease (AprA) production was interfered by these antibiotics. Extracellular proteins were not observed at the same level when Ap...
Source: Plasmid - March 19, 2016 Category: Biotechnology Source Type: research
The ancient small mobilizable plasmid pALWED1.8 harboring a new variant of the non-cassette streptomycin/spectinomycin resistance gene aadA27
Publication date: Available online 16 February 2016 Source:Plasmid Author(s): Anton Kurakov, Sofia Mindlin, Alexey Beletsky, Natalya Shcherbatova, Andrey Rakitin, Aleksandra Ermakova, Andrey Mardanov, Mayya Petrova The small mobilizable plasmid pALWED1.8 containing a novel variant of the streptomycin/spectinomycin resistance gene aadA27 was isolated from the permafrost strains of Acinetobacter lwoffii. The 4135bp plasmid carries mobА and mobC genes that mediate its mobilization by conjugative plasmids. The nucleotide sequences of mobА and mobC are similar to those of mobilization genes of the modern p...
Source: Plasmid - February 18, 2016 Category: Biotechnology Source Type: research
