A novel plasmid, pSAA0430-08, from Streptococcus anginosus subsp. anginosus strain 0430-08
Publication date: January 2018 Source:Plasmid, Volume 95 Author(s): Atsushi Tabata, Douglas Deutsch, Seiya Otsuka, Kathleen Verratti, Toshifumi Tomoyasu, Hideaki Nagamune, Vincent A. Fischetti Mobile genetic elements (MGEs) are the genetic material often involved in the interspecies and intraspecies genetic transduction in bacteria. However, little is known about MGEs in the Anginosus group of streptococci (AGS), one of the streptococcal groups found in the oral cavity of humans. We looked for the presence of MGEs in Streptococcus anginosus subsp. anginosus (SAA), a representative species belonging to AGS, and fou...
Source: Plasmid - April 14, 2018 Category: Biotechnology Source Type: research
Construction of pOGOduet – An inducible, bicistronic vector for synthesis of recombinant proteins in Corynebacterium glutamicum
Publication date: January 2018 Source:Plasmid, Volume 95 Author(s): Oliver Goldbeck, Gerd M. Seibold The Gram-positive Corynebacterium glutamicum is widely known for its application in the industrial production of amino acids and as a non-pathogenic model organism for cell wall biosynthesis in the group of CMN bacteria. For biotechnological and physiological studies often co-expression of recombinant genes is required, however for C. glutamicum no vector for the independent co-expression of two genes was described. We here created the novel expression vector pOGOduet for C. glutamicum, which carries the ColE1 replicon ...
Source: Plasmid - April 14, 2018 Category: Biotechnology Source Type: research
Comparative analysis of rhizobial chromosomes and plasmids to estimate their evolutionary relationships
Publication date: March–May 2018 Source:Plasmid, Volumes 96–97 Author(s): Xinye Wang, Dongying Liu, Yantao Luo, Liang Zhao, Zhenshan Liu, Minxia Chou, Entao Wang, Gehong Wei In the present study, complete genomic sequences retrieved from 57 rhizobial strains that covered four genera including 11 species were analyzed comprehensively. The four types of replicons: chromosomes, chromids, nonsymbiotic plasmids, and symbiotic plasmids were investigated and compared among these strains. Results showed that co-evolution occurred among these four replicons based on the similarities in average nucleotide identity. Hig...
Source: Plasmid - April 14, 2018 Category: Biotechnology Source Type: research
Compatibility and entry exclusion of IncA and IncC plasmids revisited: IncA and IncC plasmids are compatible
Publication date: March–May 2018 Source:Plasmid, Volumes 96–97 Author(s): Stephanie J. Ambrose, Christopher J. Harmer, Ruth M. Hall In an early study, IncA and IncC plasmids that were reported to be compatible were grouped as the “A-C complex” based on similarities and on strong entry exclusion. However, recently, the term IncA/C has been used frequently to describe plasmids belonging to both of these two groups. Granted that the supporting data was not included in the original reports and that the consensus iteron sequences have since been shown to be essentially identical, we have addressed the question agai...
Source: Plasmid - April 14, 2018 Category: Biotechnology Source Type: research
Complete sequence of the tumor-inducing plasmid pTiChry5 from the hypervirulent Agrobacterium tumefaciens strain Chry5
Publication date: March–May 2018 Source:Plasmid, Volumes 96–97 Author(s): Shuai Shao, Xiaorong Zhang, G. Paul H. van Heusden, Paul J.J. Hooykaas Agrobacterium tumefaciens strain Chry5 is hypervirulent on many plants including soybean that are poorly transformed by other A. tumefaciens strains. Therefore, it is considered as a preferred vector for genetic transformation of plants. Here we report the complete nucleotide sequence of its chrysopine-type Ti-plasmid pTiChry5. It is comprised of 197,268 bp with an overall GC content of 54.5%. Two T-DNA regions are present and 219 putative protein-coding sequences coul...
Source: Plasmid - April 14, 2018 Category: Biotechnology Source Type: research
Pheromone-inducible expression vectors for fission yeast Schizosaccharomyces pombe
Publication date: Available online 26 November 2017 Source:Plasmid Author(s): Stefan Hennig, Nadine Hornauer, Gerhard Rödel, Kai Ostermann The fission yeast Schizosaccharomyces pombe is an attractive host for heterologous gene expression. However, expression systems for industrially viable large-scale fermentations are scarce. Several inducible expression vectors for S. pombe have been reported, with the strong thiamine-repressible nmt1 + promoter or derivatives thereof most commonly employed. Previously, the promoter regions of the genes sxa2 + and rep1 + were utilized to couple pheromone signaling to the expressio...
Source: Plasmid - December 4, 2017 Category: Biotechnology Source Type: research
A synthetic construct for genetic engineering of the emerging pathogenic yeast Candida auris
Publication date: January 2018 Source:Plasmid, Volume 95 Author(s): Tatiana A. Defosse, Yohann Le Govic, Patrick Vandeputte, Vincent Courdavault, Marc Clastre, Jean-Philippe Bouchara, Anuradha Chowdhary, Nathalie Giglioli-Guivarc'h, Nicolas Papon Candida auris has recently emerged as a global cause of severe hospital-acquired fungal infections. To enable functional genomic approaches for this prominent pathogen, we designed a synthetic construct that can be used to genetically transform the genome-sequenced strain VPCI 479/P/13 of C. auris following an efficient electroporation procedure. (Source: Plasmid)
Source: Plasmid - December 4, 2017 Category: Biotechnology Source Type: research
Evolution in situ of ARI-A in pB2-1, a type 1 IncC plasmid recovered from Klebsiella pneumoniae, and stability of Tn4352B
Publication date: November 2017 Source:Plasmid, Volume 94 Author(s): Christopher J. Harmer, Ruth M. Hall The IncC plasmid pB2-1, from a Klebsiella pneumoniae isolate recovered in Brisbane prior to 1995, belongs to a subtype of type 1 IncC plasmids, here designated type 1a, that includes those carrying carbapenem resistance genes such as bla NDM and bla KPC. pB2-1 carries a 2358bp deletion in the rhs1 gene found in four other type 1a IncC plasmids. pB2-1 confers resistance to ampicillin, gentamicin, kanamycin, neomycin, tobramycin, sulfamethoxazole, tetracycline and trimethoprim. It transferred at a frequency of 4.7×10...
Source: Plasmid - November 19, 2017 Category: Biotechnology Source Type: research
Construction of an expression vector that uses the aph promoter for protein expression in Corynebacterium glutamicum
Publication date: Available online 3 October 2017 Source:Plasmid Author(s): Wei Zhang, Zihao Zhao, Yankun Yang, Xiuxia Liu, Zhonghu Bai Corynebacterium glutamicum is an attractive host for the production of heterologous proteins despite its traditional use in fermentative production of amino acids. To enhance the expression levels of target genes, the development of useful promoters is required in the construction of expression systems. Here, we developed a new promoter, the aph promoter from aminoglycoside-3′-phosphotransferase gene, and used it to construct monocistronic and bicistronic expression systems that h...
Source: Plasmid - October 4, 2017 Category: Biotechnology Source Type: research
Novel toxins from type II toxin-antitoxin systems with acetyltransferase activity
Publication date: Available online 20 September 2017 Source:Plasmid Author(s): Dukas Jurėnas, Abel Garcia-Pino, Laurence Van Melderen Type II toxin-antitoxin (TA) systems are widespread in bacterial and archeal genomes. These modules are very dynamic and participate in bacterial genome evolution through horizontal gene transfer. TA systems are commonly composed of a labile antitoxin and a stable toxin. Toxins appear to preferentially inhibit the protein synthesis process. Toxins use a variety of molecular mechanisms and target nearly every step of translation to achieve their inhibitory function. This review focuses ...
Source: Plasmid - September 21, 2017 Category: Biotechnology Source Type: research
Multiple plasmid interference – Pledging allegiance to my enemy's enemy
Publication date: Available online 24 August 2017 Source:Plasmid Author(s): João Alves Gama, Rita Zilhão, Francisco Dionisio As shown in the previous paper, two distinct conjugative plasmids sometimes interact within bacterial cells, implicating changes of transfer rates. In most cases of interactions within bacteria, the transfer of one of the plasmids decreases. Less frequently, the transfer rate of one of the plasmids increases. Here we analyse what happens if three distinct conjugative plasmids colonize the same bacterial cell. Our aim is to understand how interactions between two plasmids affect the transfer ra...
Source: Plasmid - August 25, 2017 Category: Biotechnology Source Type: research
Conjugation efficiency depends on intra and intercellular interactions between distinct plasmids: Plasmids promote the immigration of other plasmids but repress co-colonizing plasmids
Publication date: Available online 24 August 2017 Source:Plasmid Author(s): João Alves Gama, Rita Zilhão, Francisco Dionisio Conjugative plasmids encode the genes responsible for the synthesis of conjugative pili and plasmid transfer. Expression of the conjugative machinery (including conjugative pili) may be costly to bacteria, not only due to the energetic/metabolic cost associated with their expression but also because they serve as receptors for certain viruses. Consequently, the presence of two plasmids in the same cell may be disadvantageous to each plasmid, because they may impose a higher fitness cost on the...
Source: Plasmid - August 25, 2017 Category: Biotechnology Source Type: research
Co-resident plasmids travel together
Publication date: Available online 24 August 2017 Source:Plasmid Author(s): João Alves Gama, Rita Zilhão, Francisco Dionisio Conjugative plasmids encode genes that enable them to transfer, by conjugation, from a given host cell to another host cell. Conjugative transfer, despite being an important feature of conjugative plasmids, is not constitutive for most plasmids, the reason being that genes involved in horizontal transfer are mostly repressed. Only upon their transient de-repression are plasmids able to transfer horizontally. If host cells harbour multiple plasmids, their simultaneous transfer depends on simult...
Source: Plasmid - August 25, 2017 Category: Biotechnology Source Type: research
Molecular characterization of the pA3J1 plasmid from the psychrotolerant Antarctic bacterium Pseudomonas sp. ANT_J3
In this study, the molecular characterization of the pA3J1 plasmid of Antarctic psychrotolerant bacterium Pseudomonas sp. ANT_J3 was performed. Within this plasmid, thirteen putative open reading frames were identified. Nine of them encoded proteins involved in replication, partitioning, postsegregational elimination of plasmid-less cells (via a toxin-antitoxin system activity), multimer resolution and mobilization by conjugal transfer. These genes constitute the plasmid backbone. The functional analysis of the pA3J1 maintenance region revealed that it is a narrow host range replicon, stably maintained in the host cells by...
Source: Plasmid - August 10, 2017 Category: Biotechnology Source Type: research
A novel type 1/2 hybrid IncC plasmid carrying fifteen antimicrobial resistance genes recovered from Proteus mirabilis in China
Publication date: Available online 27 July 2017 Source:Plasmid Author(s): Chang-Wei Lei, Ling-Han Kong, Su-Zhen Ma, Bi-Hui Liu, Yan-Peng Chen, An-Yun Zhang, Hong-Ning Wang IncC plasmids are of great concern as vehicles of broad-spectrum cephalosporins and carbapenems resistance genes bla CMY and bla NDM. The aim of this study was to sequence and characterize a multidrug resistance (MDR) IncC plasmid (pPm14C18) recovered from Proteus mirabilis. pPm14C18 was identified in a CMY-2-producing P. mirabilis isolate from chicken in China in 2014, and could be transferred to Escherichia coli conferring an MDR phenotype. Wh...
Source: Plasmid - July 28, 2017 Category: Biotechnology Source Type: research
