Editorial Board
Publication date: March–May 2018Source: Plasmid, Volumes 96–97Author(s): (Source: Plasmid)
Source: Plasmid - July 6, 2018 Category: Biotechnology Source Type: research
Complete sequence of the tumor-inducing plasmid pTiChry5 from the hypervirulent Agrobacterium tumefaciens strain Chry5
Publication date: March–May 2018Source: Plasmid, Volumes 96–97Author(s): Shuai Shao, Xiaorong Zhang, G. Paul H. van Heusden, Paul J.J. HooykaasAbstractAgrobacterium tumefaciens strain Chry5 is hypervirulent on many plants including soybean that are poorly transformed by other A. tumefaciens strains. Therefore, it is considered as a preferred vector for genetic transformation of plants. Here we report the complete nucleotide sequence of its chrysopine-type Ti-plasmid pTiChry5. It is comprised of 197,268 bp with an overall GC content of 54.5%. Two T-DNA regions are present and 219 putative protein-coding sequences coul...
Source: Plasmid - July 6, 2018 Category: Biotechnology Source Type: research
Compatibility and entry exclusion of IncA and IncC plasmids revisited: IncA and IncC plasmids are compatible
Publication date: March–May 2018Source: Plasmid, Volumes 96–97Author(s): Stephanie J. Ambrose, Christopher J. Harmer, Ruth M. HallAbstractIn an early study, IncA and IncC plasmids that were reported to be compatible were grouped as the “A-C complex” based on similarities and on strong entry exclusion. However, recently, the term IncA/C has been used frequently to describe plasmids belonging to both of these two groups. Granted that the supporting data was not included in the original reports and that the consensus iteron sequences have since been shown to be essentially identical, we have addressed the question aga...
Source: Plasmid - July 6, 2018 Category: Biotechnology Source Type: research
Comparative analysis of rhizobial chromosomes and plasmids to estimate their evolutionary relationships
Publication date: March–May 2018Source: Plasmid, Volumes 96–97Author(s): Xinye Wang, Dongying Liu, Yantao Luo, Liang Zhao, Zhenshan Liu, Minxia Chou, Entao Wang, Gehong WeiAbstractIn the present study, complete genomic sequences retrieved from 57 rhizobial strains that covered four genera including 11 species were analyzed comprehensively. The four types of replicons: chromosomes, chromids, nonsymbiotic plasmids, and symbiotic plasmids were investigated and compared among these strains. Results showed that co-evolution occurred among these four replicons based on the similarities in average nucleotide identity. High co...
Source: Plasmid - July 6, 2018 Category: Biotechnology Source Type: research
Identification of large cryptic plasmids in Clostridioides (Clostridium) difficile
Publication date: March–May 2018Source: Plasmid, Volumes 96–97Author(s): Jacob Amy, Dieter Bulach, Daniel Knight, Tom Riley, Priscilla Johanesen, Dena LyrasAbstractClostridioides (Clostridium) difficile is a major bacterial pathogen of both humans and animals. Several species of pathogenic clostridia are known to harbour large plasmids with combinations of virulence, antibiotic resistance and metabolism determinants. Small cryptic plasmids have been previously identified in C. difficile, but there is a lack of recent work examining the prevalence and heterogeneity of plasmids in this diverse bacterial species. A survey...
Source: Plasmid - July 6, 2018 Category: Biotechnology Source Type: research
Mobile macrolide resistance genes in staphylococci
Publication date: Available online 26 May 2018Source: PlasmidAuthor(s): Andrea T. Feßler, Yang Wang, Congming Wu, Stefan SchwarzAbstractMacrolide resistance in staphylococci is based on the expression of a number of genes which specify four major resistance mechanisms: (i) target site modification by methylation of the ribosomal target site in the 23S rRNA, (ii) ribosome protection via ABC-F proteins, (iii) active efflux via Major Facilitator Superfamily (MFS) transporters, and (iv) enzymatic inactivation by phosphotransferases or esterases. So far, 14 different classes of erm genes, which code for 23S rRNA methylases, ha...
Source: Plasmid - July 6, 2018 Category: Biotechnology Source Type: research
Baculovirus for gene delivery to mammalian cells: Past, present and future
Publication date: June 2018Source: Plasmid, Volume 98Author(s): Maysam Mansouri, Philipp BergerAbstractBaculovirus is an insect virus which has been used for more than thirty years for production of recombinant proteins in insect cells. However, baculovirus can also be harnessed for efficient gene delivery to mammalian cells if it is equipped with mammalian promoters. This technology is known as BacMam and has been used for gene delivery to immortalized cell lines, stem cells, and primary cells, as well as for gene delivery in animals. Baculovirus has unique features when compared to mammalian viruses. Besides the fact tha...
Source: Plasmid - July 6, 2018 Category: Biotechnology Source Type: research
Mobile lincosamide resistance genes in staphylococci
Publication date: Available online 19 June 2018Source: PlasmidAuthor(s): Andrea T. Feßler, Yang Wang, Congming Wu, Stefan SchwarzAbstractLincosamide resistance in staphylococci is based on the expression of a number of genes which specify three major resistance mechanisms: (i) enzymatic inactivation by lincosamide nucleotidyltransferases, (ii) ribosome protection by ABC-F proteins, and (iii) methylation of the ribosomal target sites in the 23S rRNA by Cfr or Erm methylases. So far, only two lnu genes, lnu(A) and lnu(B), which code for different types of lincosamide nucleotidyltransferases, have been found in staphylococci...
Source: Plasmid - July 6, 2018 Category: Biotechnology Source Type: research
Broad-host-range Inc18 plasmids: Occurrence, spread and transfer mechanisms
Publication date: Available online 19 June 2018Source: PlasmidAuthor(s): Verena Kohler, Ankita Vaishampayan, Elisabeth GrohmannAbstractConjugative plasmid transfer is one of the major mechanisms responsible for the spread of antibiotic resistance and virulence genes. The incompatibility (Inc) 18 group of plasmids is a family of plasmids replicating by the theta-mechanism, whose members have been detected frequently in enterococci and streptococci. Inc18 plasmids encode a variety of antibiotic resistances, including resistance to vancomycin, chloramphenicol and the macrolide-lincosamide-streptogramine (MLS) group of antibio...
Source: Plasmid - July 6, 2018 Category: Biotechnology Source Type: research
Broad-host-range Inc18 plasmids: Occurrence, spread and transfer mechanisms
Publication date: Available online 19 June 2018 Source:Plasmid Author(s): Verena Kohler, Ankita Vaishampayan, Elisabeth Grohmann Conjugative plasmid transfer is one of the major mechanisms responsible for the spread of antibiotic resistance and virulence genes. The incompatibility (Inc) 18 group of plasmids is a family of plasmids replicating by the theta-mechanism, whose members have been detected frequently in enterococci and streptococci. Inc18 plasmids encode a variety of antibiotic resistances, including resistance to vancomycin, chloramphenicol and the macrolide-lincosamide-streptogramine (MLS) group of antibiot...
Source: Plasmid - June 20, 2018 Category: Biotechnology Source Type: research
Mobile lincosamide resistance genes in staphylococci
Publication date: Available online 19 June 2018 Source:Plasmid Author(s): Andrea T. Feßler, Yang Wang, Congming Wu, Stefan Schwarz Lincosamide resistance in staphylococci is based on the expression of a number of genes which specify three major resistance mechanisms: (i) enzymatic inactivation by lincosamide nucleotidyltransferases, (ii) ribosome protection by ABC-F proteins, and (iii) methylation of the ribosomal target site in the 23S rRNA by Cfr or Erm methylases. So far, only two lnu genes, lnu(A) and lnu(B), which code for different types of lincosamide nucleotidyltransferases have been found in staphylococci. ...
Source: Plasmid - June 20, 2018 Category: Biotechnology Source Type: research
Baculovirus for gene delivery to mammalian cells: Past, present and future
Publication date: Available online 26 May 2018 Source:Plasmid Author(s): Maysam Mansouri, Philipp Berger Baculovirus is an insect virus which has been used for more than thirty years for production of recombinant proteins in insect cells. However, baculovirus can also be harnessed for efficient gene delivery to mammalian cells if it is equipped with mammalian promoters. This technology is known as BacMam and has been used for gene delivery to immortalized cell lines, stem cells, and primary cells, as well as for gene delivery in animals. Baculovirus has unique features when compared to mammalian viruses. Besides the fa...
Source: Plasmid - May 27, 2018 Category: Biotechnology Source Type: research
Mobile macrolide resistance genes in staphylococci
Publication date: Available online 26 May 2018 Source:Plasmid Author(s): Andrea T. Feßler, Yang Wang, Congming Wu, Stefan Schwarz Macrolide resistance in staphylococci is based on the expression of a number of genes which specify three major resistance mechanisms: (i) target site modification by methylation of the ribosomal target site in the 23S rRNA, (ii) active efflux via ABC transporters or Major Facilitator Superfamily (MFS) transporters, and (iii) enzymatic inactivation by phosphotransferases or esterases. So far, 14 different classes of erm genes, which code for 23S rRNA methylases, have been reported to occu...
Source: Plasmid - May 26, 2018 Category: Biotechnology Source Type: research
Identification of large cryptic plasmids in Clostridioides (Clostridium) difficile
Publication date: March–May 2018 Source:Plasmid, Volumes 96–97 Author(s): Jacob Amy, Dieter Bulach, Daniel Knight, Tom Riley, Priscilla Johanesen, Dena Lyras Clostridioides (Clostridium) difficile is a major bacterial pathogen of both humans and animals. Several species of pathogenic clostridia are known to harbour large plasmids with combinations of virulence, antibiotic resistance and metabolism determinants. Small cryptic plasmids have been previously identified in C. difficile, but there is a lack of recent work examining the prevalence and heterogeneity of plasmids in this diverse bacterial species. A surv...
Source: Plasmid - May 15, 2018 Category: Biotechnology Source Type: research
Environmental Mycobacterium avium subsp. hominissuis have a higher probability to act as a recipient in conjugation than clinical strains
Publication date: January 2018 Source:Plasmid, Volume 95 Author(s): Salma A. Shoulah, Anna M. Oschmann, Abdelfattah Selim, Torsten Semmler, Carsten Schwarz, Elisabeth Kamal, Faysal Hamouda, Elsayed Galila, Wilbert Bitter, Astrid Lewin Mycobacterium avium subsp. hominissuis (MAH) is a widespread opportunistic pathogen that can be isolated from environment (dust, soil and water) and patients with lung or lymphnode infection. In our previous research we revealed the pronounced genetic diversity in MAH by identifying eight different types of a newly described genomic island. In order to identify mechanisms of such ...
Source: Plasmid - April 14, 2018 Category: Biotechnology Source Type: research
