pCERC3 from a commensal ST95 Escherichia coli: A ColV virulence-multiresistance plasmid carrying a sul3-associated class 1 integron
Publication date: March–May 2016 Source:Plasmid, Volumes 84–85 Author(s): Robert A. Moran, Kathryn E. Holt, Ruth M. Hall The rare sulphonamide resistance gene sul3 was found in the commensal Escherichia coli ST95 strain 22.1-R1 that was isolated in 2010 from the faeces of a healthy Australian adult. The genome of 22.1-R1 was sequenced and a 144,344bp RepFII/FIB plasmid, pCERC3, carrying sul3 was assembled. The sul3 gene is part of a class 1 integron featuring a sul3-containing conserved segment (sul3-CS) that replaced the classic sul1-containing 3′-conserved segment (3′-CS) usually seen in class 1 integro...
Source: Plasmid - February 18, 2016 Category: Biotechnology Source Type: research
pNEB193-derived suicide plasmids for gene deletion and protein expression in the methane-producing archaeon, Methanosarcina acetivorans
Publication date: Available online 11 February 2016 Source:Plasmid Author(s): Mitchell T. Shea, Mary E. Walter, Nikolas Duszenko, Anne-Lise Ducluzeau, Jared Aldridge, Shannon K. King, Nicole R. Buan Gene deletion and protein expression are cornerstone procedures for studying metabolism in any organism, including methane-producing archaea (methanogens). Methanogens produce coenzymes and cofactors not found in most bacteria, therefore it is sometimes necessary to express and purify methanogen proteins from the natural host. Protein expression in the native organism is also useful when studying post-translat...
Source: Plasmid - February 11, 2016 Category: Biotechnology Source Type: research
pBR322 vectors having tetracycline-dependent replication
In this report pAM34 has been modified by replacement of the lac regulatory elements with those of the transposon Tn10 tetracycline resistance module. This resulted in medium copy number plasmids that are dependent on the presence of tetracycline (or less satisfactorily, anhydrotetracycline) for replication. The tetracycline-dependent plasmids are rapidly lost in the absence of tetracycline and plasmid loss is markedly accelerated when the host strain expresses a tetracycline efflux pump. (Source: Plasmid)
Source: Plasmid - February 11, 2016 Category: Biotechnology Source Type: research
Sequence analysis of five endogenous plasmids isolated from Lactobacillus pentosus F03
Publication date: Available online 4 February 2016 Source:Plasmid Author(s): Lin-Shu Kung, Ming-Te Yang, Jyh-Shiun Lin Lactobacillus pentosus F03, a strain isolated from pig intestines in Taiwan, contains multiple endogenous plasmids. We isolated, completely sequenced, and characterized five of the plasmids present in L. pentosus F03 designated as pF03–1 (3282bp), pF03–2 (3293bp), pF03–3 (1787bp), pF03–4 (2138bp), and pF03–5 (1949bp). The replication types of these plasmids were predicted by comparing the features of the replicon nucleotides and the similarity of replication proteins with those of the ...
Source: Plasmid - February 4, 2016 Category: Biotechnology Source Type: research
Insect cell transformation vectors that support high level expression and promoter assessment in insect cell culture
Publication date: January 2016 Source:Plasmid, Volume 83 Author(s): Paul D. Shirk, Richard B. Furlong A somatic transformation vector, pDP9, was constructed that provides a simplified means of producing permanently transformed cultured insect cells that support high levels of protein expression of foreign genes. The pDP9 plasmid vector incorporates DNA sequences from the Junonia coenia densovirus that are involved in integration of the densovirus in insect cell chromosomes and a promoter/enhancer system that results in high levels of expression. The plasmid also contains two markers that permit selection of transfo...
Source: Plasmid - January 24, 2016 Category: Biotechnology Source Type: research
Insect cell transformation vectors that supports high level expression and promoter assessment in insect cell culture
Publication date: Available online 13 January 2016 Source:Plasmid Author(s): Paul D. Shirk, Richard B. Furlong A somatic transformation vector, pDP9, was constructed that provides a simplified means of producing permanently transformed cultured insect cells that support high levels of protein expression of foreign genes. The pDP9 plasmid vector incorporates DNA sequences from the Junonia coenia densovirus that are involved in integration of the densovirus in insect cell chromosomes and a promoter/enhancer system that results in high levels of expression. The plasmid also contains two markers that permit selection o...
Source: Plasmid - January 13, 2016 Category: Biotechnology Source Type: research
Whole genome sequencing of diverse Shiga toxin-producing and non-producing Escherichia coli strains reveals a variety of virulence and novel antibiotic resistance plasmids
Publication date: Available online 31 December 2015 Source:Plasmid Author(s): Liliana Losada, Chitrita DebRoy, Diana Radune, Maria Kim, Ravi Sanka, Lauren Brinkac, Subhashinie Kariyawasam, Daniel Shelton, Pina M. Fratamico, Vivek Kapur, Peter C.H. Feng The genomes of a diverse set of Escherichia coli, including many Shiga toxin-producing strains of various serotypes were determined. A total of 39 plasmids were identified among these strains, and many carried virulence or putative virulence genes of Shiga toxin-producing E. coli strains, virulence genes for other pathogenic E. coli groups, and some...
Source: Plasmid - January 11, 2016 Category: Biotechnology Source Type: research
Sequence analysis and minimal replicon determination of a new haloarchaeal plasmid pHF2 isolated from Haloferax sp. strain Q22
Publication date: Available online 19 November 2015 Source:Plasmid Author(s): Shaoxing Chen, Chuangming Wang, Hua Xiang A new cryptic plasmid, pHF2 (2520bp), was isolated from Haloferax sp. strain Q22 (=CGMCC 1.15317), a haloarchaeal strain living in a subterranean halite deposit. Sequence analysis revealed that it is the smallest plasmid in the genus Haloferax so far, and three syntropic open reading frames (ORF1, ORF2, and ORF3) were identified on the same strand. ORF1 encodes a putative replication initiation protein (Rep). Three typical motifs (I, II, and III) were presented in the Rep proteins of rolling-cir...
Source: Plasmid - November 22, 2015 Category: Biotechnology Source Type: research
Strain ŽP — the first bacterial conjugation-based “kill”–“anti-kill” antimicrobial system
Publication date: November 2015 Source:Plasmid, Volume 82 Author(s): Marjanca Starčič Erjavec, Živa Petkovšek, Marina V. Kuznetsova, Irina L. Maslennikova, Darja Žgur-Bertok As multidrug resistant bacteria pose one of the greatest risks to human health new alternative antibacterial agents are urgently needed. One possible mechanism that can be used as an alternative to traditional antibiotic therapy is transfer of killing agents via conjugation. Our work was aimed at providing a proof of principle that conjugation-based antimicrobial systems are possible. We constructed a bacterial conjugation-based “k...
Source: Plasmid - October 30, 2015 Category: Biotechnology Source Type: research
Construction of pDYN6902, a new Streptomyces integrative expression vector designed for cloning sequences interfering with Escherichia coli viability
Publication date: Available online 22 October 2015 Source:Plasmid Author(s): Annabelle Thibessard, Claire Bertrand, Julien Hiblot, Emilie Piotrowski, Pierre Leblond (Source: Plasmid)
Source: Plasmid - October 28, 2015 Category: Biotechnology Source Type: research
Construction of a novel twin-arginine translocation (Tat)-dependent type expression vector for secretory production of heterologous proteins in Corynebacterium glutamicum
In this study, we constructed a shuttle expression vector pAU3, which harbors the strong promoter tac-M for constitutive gene transcription, the consensus RBS sequence for protein translation, and the strong cgR_0949 signal sequence for protein secretion via the Tat pathway in C. glutamicum. The applicability of pAU3 was confirmed by the highly efficient expression and secretion of the CAT protein in C. glutamicum. The vector pAU3 is highly useful for secretory production of heterologous proteins in Corynebacterium glutamicum. Graphical abstract (Source: Plasmid)
Source: Plasmid - October 28, 2015 Category: Biotechnology Source Type: research
Development of an anhydrotetracycline-inducible expression system for expression of a neopullulanase in B. subtilis
In this study, we developed a robust expression system based on optimized P tetR of transposon Tn1721, which is repressible by its specific repressor, TetR. The first step of this work was focused on the optimization of structure and core elements of Tn1721 anhydrotetracycline-inducible promoters, P tetA and P tetR . Both promoters were inserted upstream of eGFP on a pUB110-derivative with high copy number. Reduction of the 18bp spacer region of both P tetA and P tetR to 17bp significantly increased their strength in B. subtilis. Nevertheless, only the optimized P tetR with 17bp spacer region (P tetR2) directed high l...
Source: Plasmid - October 10, 2015 Category: Biotechnology Source Type: research
Strain ŽP – the first bacterial conjugation-based »kill« – »anti-kill« antimicrobial system
Publication date: Available online 3 October 2015 Source:Plasmid Author(s): Marjanca Starčič Erjavec, Živa Petkovšek, Marina V. Kuznetsova, Irina L. Maslennikova, Darja Žgur-Bertok As multidrug resistant bacteria pose one of the greatest risks to human health new alternative antibacterial agents are urgently needed. One possible mechanism that can be used as an alternative to traditional antibiotic therapy is transfer of killing agents via conjugation. Our work was aimed at providing a proof of principle that conjugation-based antimicrobial systems are possible. We constructed a bacterial conjugation-bas...
Source: Plasmid - October 5, 2015 Category: Biotechnology Source Type: research
Identification, sequencing and comparative analysis of pBp15.S plasmid from the newly described entomopathogen Bacillus pumilus 15.1
Publication date: Available online 28 September 2015 Source:Plasmid Author(s): Diana C. Garcia-Ramon, Maria Jose Luque-Navas, C. Alfonso Molina, Coral del Val, Antonio Osuna, Susana Vilchez The Bacillus pumilus 15.1 strain, a recently described entomopathogenic strain active against Ceratitis capitata, contains at least two extrachromosomal elements, pBp15.1S and pBp15.1B. Given that B. pumilus is not a typical entomopathogenic bacterium, the acquisition of this extrachromosomal DNA may explain why B. pumilus 15.1 is toxic to an insect. One of the plasmids present in the strain, the pBp15.1S plasmid, was su...
Source: Plasmid - September 29, 2015 Category: Biotechnology Source Type: research
The antisense leitmotif: A prelude
Publication date: March 2015 Source:Plasmid, Volume 78 Author(s): M. Pilar Garcillán-Barcia, Manuel Espinosa (Source: Plasmid)
Source: Plasmid - August 13, 2015 Category: Biotechnology Source Type: research
