Editorial Board
Publication date: June 2018Source: Plasmid, Volume 98Author(s): (Source: Plasmid)
Source: Plasmid - October 5, 2018 Category: Biotechnology Source Type: research
Linezolid resistance genes and genetic elements enhancing their dissemination in enterococci and streptococci
Publication date: Available online 22 September 2018Source: PlasmidAuthor(s): Ewa SadowyAbstractLinezolid is considered a last resort drug in treatment of severe infections caused by Gram-positive pathogens, resistant to other antibiotics, such as vancomycin-resistant enterococci (VRE), methicillin-resistant staphylococci and multidrug resistant pneumococci. Although the vast majority of Gram-positive pathogenic bacteria remain susceptible to linezolid, resistant isolates of enterococci, staphylococci and streptococci have been reported worldwide. In these bacteria, apart from mutations, affecting mostly the 23S rRNA genes...
Source: Plasmid - October 5, 2018 Category: Biotechnology Source Type: research
Plasmids for making multiple knockouts in a radioresistant bacterium Deinococcus radiodurans
Publication date: November 2018Source: Plasmid, Volume 100Author(s): Ganesh K. Maurya, Hari S. MisraAbstractThe gene knockouts are mostly created using homologous recombination-based replacement of target gene(s) with the expressing cassette of selection marker gene(s). Here, we constructed a series of plasmids bearing the expressing cassettes of genes encoding different antibiotics markers like nptII (KanR), aadA (SpecR), cat (CmR) and aac(3) (GenR). D. radiodurans is a radioresistant Gram positive bacterium that does not support the independent maintenance of colE1 origin-based plasmids. Using these constructs, the disru...
Source: Plasmid - October 5, 2018 Category: Biotechnology Source Type: research
An IncX1 plasmid isolated from Salmonella enterica subsp. enterica serovar Pullorum carrying blaTEM-1B, sul2, arsenic resistant operons
Publication date: Available online 21 September 2018Source: PlasmidAuthor(s): Xiu-Zhong Zhang, Chang-Wei Lei, Jin-Xin Zeng, Yan-Peng Chen, Zhuang-Zhuang Kang, Yu-Long Wang, Xiao-Lan Ye, Xi-Wen Zhai, Hong-Ning WangAbstractWe have identified an IncX1 plasmid named pQJDSal1 from Salmonella enterica subsp. enterica serovar Pullorum (S. Pullorum). The plasmid is 67,685 bp in size and has 72 putative genes. pQJDSal1 harbors a conserved IncX1-type backbone with predicted regions for conjugation, replication and partitioning, as well as a toxin/antitoxin plasmid addiction system. Two regions (A and B) that have not been previous...
Source: Plasmid - September 22, 2018 Category: Biotechnology Source Type: research
Plasmid-mediated resistance is going wild
Publication date: Available online 21 September 2018Source: PlasmidAuthor(s): Monika Dolejska, Costas C. PapagiannitsisAbstractMultidrug resistance (MDR) Gram-negative bacteria have been increasingly reported in humans, companion animals and farm animals. The growing trend of plasmid-mediated resistance to antimicrobial classes of critical importance is attributed to the emergence of epidemic plasmids, rapidly disseminating resistance genes among the members of Enterobacteriaceae family. The use of antibiotics to treat humans and animals has had a significant impact on the environment and on wild animals living and feeding...
Source: Plasmid - September 22, 2018 Category: Biotechnology Source Type: research
Impact of plasmid interactions with the chromosome and other plasmids on the spread of antibiotic resistance
Publication date: Available online 19 September 2018Source: PlasmidAuthor(s): João Alves Gama, Rita Zilhão, Francisco DionisioAbstractNaturally occurring plasmids have medical importance given that they frequently code for virulence or antibiotic resistance. In many cases, plasmids impose a fitness cost to their hosts, meaning that the growth rate of plasmid-bearing cells is lower than that of plasmid-free cells. However, this does not fit with the fact that plasmids are ubiquitous in nature nor that plasmids and their hosts adapt to each other very fast – as has been shown in laboratory evolutionary assays. Even when ...
Source: Plasmid - September 20, 2018 Category: Biotechnology Source Type: research
Identification and sequence analysis of two novel cryptic plasmids isolated from the vaginal mucosa of South African women
Publication date: Available online 18 September 2018Source: PlasmidAuthor(s): Lyle Harris, Leonardo J. van Zyl, Bronwyn M. Kirby-McCullough, Leonard H. Damelin, Caroline T. Tiemessen, Marla TrindadeAbstractThe vaginal mucosa is dominated by Gram positive, rod shaped lactobacilli which serve as a natural barrier against infection. In both healthy- and bacterial vaginosis (BV)-infected women Lactobacillus crispatus and Lactobacillus jensenii have been found to be the predominant Lactobacillus species. Many studies have been conducted to assess factors influencing lactobacilli dominance in the vaginal microbiome. In the prese...
Source: Plasmid - September 19, 2018 Category: Biotechnology Source Type: research
Generation of a synthetic binary plasmid that confers resistance to nourseothricin for genetic engineering of Sporothrix schenckii
Publication date: Available online 17 September 2018Source: PlasmidAuthor(s): Alma K. Tamez-Castrellón, Ricardo Romo-Lucio, Iván Martínez-Duncker, Héctor M. Mora-MontesAbstractSome members of the Sporothrix genus can cause sporotrichosis, a worldwide distributed mycosis that affects several mammalian species, including human beings. Sporothrix schenckii and Sporothrix brasiliensis are the fungal species frequently associated with this disease, and the latter has gained significant interest because of the increased number of cases associated with transmission by cats. Despite the relevance of these organisms in the medi...
Source: Plasmid - September 18, 2018 Category: Biotechnology Source Type: research
Multi-target plasmid controls for conventional and real-time PCR-based serotyping of Streptococcus pneumoniae
ConclusionsThese quantifiable multi-target plasmids simplify the preparation of controls for PCR-based serotyping of S. pneumoniae, and methods herein could be extended to other highly multiplexed PCR assays. (Source: Plasmid)
Source: Plasmid - September 12, 2018 Category: Biotechnology Source Type: research
Construction and identification of the recombinant hFcεRIα/RBL-2H3 cells
Publication date: Available online 10 September 2018Source: PlasmidAuthor(s): Zhongcheng Liu, Lifang Hao, Nanan Wang, Su Zhang, Nan Zhang, Zhenzhen Xu, Yanlei Yang, Yanfen ZhangAbstractIgE/FcεRI signal pathway plays a crucial role in triggering allergic reactions, and there is no cross-recognition between IgE and FcεRI in human and rats. In order to obtain the hFcεRIα/ RBL-2H3 cell line, total RNA was extracted from U937 cells, and the human FcεRIα gene was obtained by RT-PCR technology. Then the amplified product was digested and inserted into the pIRES2-EGFP vector. After the plasmid was transfected into the RBL-2H...
Source: Plasmid - September 11, 2018 Category: Biotechnology Source Type: research
Development of a Gateway-compatible pCAMBIA binary vector for RNAi-mediated gene knockdown in plants
Publication date: Available online 8 September 2018Source: PlasmidAuthor(s): Deshui Yu, Libing Liao, Yi Zhang, Kedong Xu, Ju Zhang, Kun Liu, Xiaoli Li, Guangxuan Tan, Jurui Zheng, Yong He, Changling Xu, Jinjin Zhao, Beibei Fu, Jiaxing Xie, Jie Mao, Chengwei LiAbstractRNA interference (RNAi), based on hairpin RNA (hpRNA) expression, plays an important role in functional analysis of plant genes. Traditional methods for making RNAi constructs usually involve multiple time-consuming cloning steps. We have developed a Gateway-compatible binary vector for RNAi-mediated gene knockdown in plants from pCAMBIA2301 and pHANNIBAL vect...
Source: Plasmid - September 8, 2018 Category: Biotechnology Source Type: research
A genome editing vector that enables easy selection and identification of knockout cells
In this report, we show that our novel vector, pGedit, significantly facilitates genome editing. (Source: Plasmid)
Source: Plasmid - September 7, 2018 Category: Biotechnology Source Type: research
Antimicrobial resistance plasmid reservoir in food and food-producing animals
Publication date: Available online 6 September 2018Source: PlasmidAuthor(s): Jean-Yves Madec, Marisa HaenniAbstractAntimicrobial resistance (AMR) plasmids have been recognized as important vectors for efficient spread of AMR phenotypes. The food reservoir includes both food-producing animals and food products, and a huge diversity of AMR plasmids have been reported in this sector. Based on molecular typing methods and/or whole genome sequencing approaches, certain AMR genes/plasmids combinations were found more frequently in food compared to other settings. However, the food source of a definite AMR plasmid is highly compl...
Source: Plasmid - September 6, 2018 Category: Biotechnology Source Type: research
Thinking outside the (Pill) box: Does toxic metal exposure thwart antibiotic stewardship best practices?
Publication date: Available online 4 September 2018Source: PlasmidAuthor(s): Anne O. Summers, Andrew G. Wiggins, Stephen P. LaVoie, Joy Wireman, Anne O. Summers (Source: Plasmid)
Source: Plasmid - September 6, 2018 Category: Biotechnology Source Type: research
A new vector for heterologous gene expression in Escherichia coli with increased stability in the absence of antibiotic
Publication date: Available online 5 September 2018Source: PlasmidAuthor(s): Fara Amelia Primelles Eguia, Henrique Roman Ramos, Stefanie Kraschowetz, Daniel Omote, Celso Raul Romero Ramos, Paulo Lee Ho, Eneas Carvalho, Viviane Maimoni GonçalvesAbstractExpression vectors for industrial production should be stable and allow tight control of protein synthesis. This is necessary to ensure plasmid transmission to daughter cells in order to achieve a stable population capable of synthesizing high amounts of the target protein. A high-copy-number plasmid, pAE, was previously used for laboratory-scale production of recombinant hu...
Source: Plasmid - September 6, 2018 Category: Biotechnology Source Type: research
