Construction and evaluation of an adenoviral vector for the liver-specific expression of the serine/arginine-rich splicing factor, SRSF3
This report details the construction of the albumin promoter-driven adenoviral shuttle vector, termed pmAlbAd5-FLAG.SRSF3, that can be used to generate functional adenovirus to express FLAG-SRSF3 specifically in liver. This vector would be suitable for over-expression of other splicing factors that could inhibit virus production. In addition, this vector would allow only liver-specific expression of other cargo genes when used in a whole-animal paradigm. (Source: Plasmid)
Source: Plasmid - August 2, 2015 Category: Biotechnology Source Type: research
The Xis2d protein of CTnDOT binds to the intergenic region between the mob and tra operons
Publication date: Available online 23 July 2015 Source:Plasmid Author(s): Crystal M. Hopp, Jeffrey F. Gardner, Abigail A. Salyers CTnDOT is a 65kbp integrative and conjugative element (ICE) that carries genes encoding both tetracycline and erythromycin resistances. The Excision operon of this element encodes Xis2c, Xis2d, and Exc proteins involved in the excision of CTnDOT from host chromosomes. These proteins are also required in the complex transcriptional regulation of the divergently transcribed transfer (tra) and mobilization (mob) operons of CTnDOT. Transcription of the tra operon is positively regulated by...
Source: Plasmid - July 24, 2015 Category: Biotechnology Source Type: research
Development of a new pCAMBIA binary vector using GATEWAY® technology
Publication date: Available online 23 July 2015 Source:Plasmid Author(s): Julie Leclercq, Toth Szabolcs, Florence Martin, Pascal Montoro pCAMBIA vectors have become popular for their easy handling, stability and the existence of a range of selection and reporter genes. However, these vectors have yet to integrate the Gateway® cloning system, which has enabled site-specific recombination without the need for restriction enzymes and ligases. This paper set out to convert the pCambia2300 binary vector into a destination vector with the Gateway® cassette driven by the CaMV35S promoter. The destination vector, pCa...
Source: Plasmid - July 24, 2015 Category: Biotechnology Source Type: research
A novel gateway-compatible binary vector series (PC-GW) for flexible cloning of multiple genes for genetic transformation of plants
Publication date: Available online 15 July 2015 Source:Plasmid Author(s): Jyoti Dalal, Roopa Yalamanchili, Christophe La Hovary, Mikyoung Ji, Maria Rodriguez-Welsh, Denise Aslett, Sowmya Ganapathy, Amy Grunden, Heike Sederoff, Rongda Qu The rapidly advancing field of plant synthetic biology requires transforming plants with multiple genes. This has sparked a growing interest in flexible plant transformation vectors, which can be used for multi-gene transformations. We have developed a novel binary vector series, named the PC-GW series (GenBank: KP826769-KP826773), for Agrobacterium-mediated plant tr...
Source: Plasmid - July 16, 2015 Category: Biotechnology Source Type: research
Transformation of Shewanella baltica with ColE1-like and P1 plasmids and their maintenance during bacterial growth in cultures
Publication date: Available online 10 July 2015 Source:Plasmid Author(s): Klaudia Milewska , Grzegorz Węgrzyn , Agnieszka Szalewska-Pałasz Presence of natural plasmids has been reported for many Shewanella isolates. However, knowledge about plasmid replication origin and segregation mechanisms is not extensive for this genus. Shewanella baltica is an important species in the marine environment due to its denitrification ability in oxygen-deficient zones and the potential role in bioremediation processes. However, no information about possible use of plasmid vectors in this species has been reported to date. Here w...
Source: Plasmid - July 11, 2015 Category: Biotechnology Source Type: research
Inhibitory effect of UvrD and DinG on the replication of ColE1-derived plasmids in Escherichia coli
Publication date: Available online 2 July 2015 Source:Plasmid Author(s): Nalae Kang , Eunsil Choi , Sung-Gun Kim , Jihwan Hwang CspA has been identified as a major cold-shock protein in E. coli. CspA binds to RNAs which are abnormally folded at low temperature and then acts as an RNA chaperone unfolding those RNAs. The dramatic expression of cspA at low temperature is contributed by posttranscriptional stability and robust translatability. Interestingly, when cspA mRNA encoding a premature nonsense codon was overexpressed at low temperature, cell growth was completely inhibited. This phenotype was termed LACE (the ...
Source: Plasmid - July 2, 2015 Category: Biotechnology Source Type: research
Curing a Large Endogenous Plasmid by Single Substitution of a Partitioning Gene
Publication date: Available online 27 June 2015 Source:Plasmid Author(s): Ningning Song , Jie Xu , Zhaoli Li , Jean-Pierre Hernalsteens To investigate whether plasmid-free cells of pathogenic E. coli can be isolated by disrupting a single gene in an endogenous plasmid without further treatment, the effect of the disruption of partitioning genes on the inheritance of the endogenous plasmid pUTI89 of the uropathogenic E. coli strain UTI89 was studied. We found that mutation of parB, which encodes a type Ib partitioning protein, could cause loss of the endogenous plasmid at a ratio of about one percent. Clones derived...
Source: Plasmid - June 29, 2015 Category: Biotechnology Source Type: research
Transcriptome modulations due to A/C2 plasmid acquisition
In this study, RNA-Seq was performed in six different strains, spanning three species, to characterize the influence of host background on the A/C2 plasmid transcriptome. In five of these strains, chromosomal transcriptomes were compared in the presence and absence of A/C2 plasmid pAR060302. Host-specific effects on plasmid gene expression were identified, and acquisition of pAR060302 resulted in changes in the expression of chromosomal genes involved in metabolism and energy production. These results suggest that A/C2 plasmid fitness is, in part, dependent on host chromosome content, as well as environmental factors. (Source: Plasmid)
Source: Plasmid - June 13, 2015 Category: Biotechnology Source Type: research
Characterization of the Lactobacillus plantarum plasmid pCD033 and generation of the plasmid free strain L. plantarum 3NSH
Publication date: Available online 31 May 2015 Source:Plasmid Author(s): Silvia Heiss , Reingard Grabherr , Stefan Heinl Lactobacillus plantarum CD033, a strain isolated from grass silage in Austria, harbours a 7.9 kb plasmid designated pCD033. Sequence analysis identified 14 open reading frames and 8 of these were supposed to be putative coding sequences. Gene annotation revealed no putative essential genes being plasmid encoded, but a plasmid addiction system based on a PemI/PemK-like toxin-antitoxin system, able to stabilize plasmid maintenance. Absence of a replication initiation protein, a double strand origin...
Source: Plasmid - May 31, 2015 Category: Biotechnology Source Type: research
A novel suicide plasmid for efficient gene mutation in Listeria monocytogenes
Publication date: Available online 31 May 2015 Source:Plasmid Author(s): Hossam Abdelhamed , Mark L. Lawrence , Attila Karsi Although several plasmids have been used in Listeria monocytogenes for generating mutants by allelic exchange, construction of L. monocytogenes mutants has been inefficient due to lack of effective selection markers for first and second recombination events. To address this problem, we have developed a new suicide plasmid, pHoss1, by using the pMAD plasmid backbone and anhydrotetracycline selection marker (secY antisense RNA) driven by an inducible Pxyl/tetO promoter. Expression of the secY an...
Source: Plasmid - May 31, 2015 Category: Biotechnology Source Type: research
An Inducible Expression System for High-Level Expression of Recombinant Proteins in Slow Growing Mycobacteria
This study demonstrated that high levels of native-like recombinant mycobacterial proteins can be produced in mycobacterial hosts, and this may aid the analysis of mycobacterial protein function and the development of new treatments. (Source: Plasmid)
Source: Plasmid - May 27, 2015 Category: Biotechnology Source Type: research
A Series of Medium and High Copy Number Arabinose-Inducible Escherichia coli Expression Vectors Compatible with pBR322 and pACYC184
We report a series of pBAD vectors that replicate using the origin of plasmid RSF1030 that are compatible with pMB1 (ColE1) and p15A (pACYC) vectors. Both high (≥pBAD24) and medium (~pBAD322) copy number plasmids encoding resistance to ampicillin, chloramphenicol, kanamycin, tetracycline, spectinomycin/streptomycin, gentamycin, or trimethoprim are available. (Source: Plasmid)
Source: Plasmid - May 27, 2015 Category: Biotechnology Source Type: research
Different IncI1 plasmids from Escherichia coli carry IS Ecp1-blaCTX-M-15 associated with different Tn 2 -derived elements
Publication date: Available online 27 April 2015 Source:Plasmid Author(s): Zhiyong Zong , Andrew N. Ginn , Hana Dobiasova , Jonathan R. Iredell , Sally R. Partridge The bla CTX-M-15 gene, encoding the globally dominant CTX-M-15 extended-spectrum β-lactamase, has generally been found in a 2.971-kb ISEcp1-bla CTX-M-15-orf477Δ transposition unit, with ISEcp1 providing a promoter. In available IncF plasmid sequences from Escherichia coli, this transposition unit interrupts a truncated copy of transposon Tn2 that lies within larger multiresistance regions. In E. coli, bla CTX-M-15 is also commonly associated with Inc...
Source: Plasmid - May 12, 2015 Category: Biotechnology Source Type: research
Tn6026 and Tn6029 are found in complex resistance regions mobilised by diverse plasmids and chromosomal islands in multiple antibiotic resistant Enterobacteriaceae
Publication date: Available online 24 April 2015 Source:Plasmid Author(s): Cameron Reid , Piklu Roy Chowdhury , Steven P. Djordjevic Transposons flanked by direct copies of IS26 are important contributors to the evolution of multiple antibiotic resistance. Tn6029 and Tn6026 are examples of composite transposons that have become widely disseminated on small and large plasmids with different incompatibility markers in pathogenic and commensal Escherichia coli and various serovars of Salmonella enterica. Some of the plasmids that harbour these transposons also carry combinations of virulence genes. Recently, Tn6029 and...
Source: Plasmid - May 7, 2015 Category: Biotechnology Source Type: research
Characterization of a collection of plasmid-containing bacteria isolated from an on-farm biopurification system used for pesticide removal
Publication date: Available online 6 May 2015 Source:Plasmid Author(s): Martini María Carla , Albicoro Francisco Javier , Nour Eman , Schlüter Andreas , van Elsas Jan Dirk , Springael Dirk , Smalla Kornellia , Pistorio Mariano , Lagares Antonio , Del Papa María Florencia Biopurification systems (BPS) are complex soil-related and artificially-generated environments usually designed for the removal of toxic compounds from contaminated wastewaters. The present study has been conducted to isolate and characterize a collection of cultivable plasmid-carrying bacterial isolates recovered from a BPS established fo...
Source: Plasmid - May 7, 2015 Category: Biotechnology Source Type: research
