Novel plasmid conferring kanamycin and tetracycline resistance in the Turkey-derived Campylobacter jejuni strain 11601MD

Publication date: Available online 4 June 2016 Source:Plasmid Author(s): M.D. Crespo, E. Altermann, J. Olson, W.G. Miller, K. Chandrashekhar, S. Kathariou In Campylobacter spp., resistance to the antimicrobials kanamycin and tetracycline is frequently associated with plasmid-borne genes. However, relatively few plasmids of Campylobacter jejuni have been fully characterized to date. A novel plasmid (p11601MD; 44,095nt) harboring tet(O) was identified in C. jejuni strain 11601MD, which was isolated from the jejunum of a turkey produced conventionally in North Carolina. Analysis of the p11601MD sequence revealed the presence of a high-GC content cassette with four genes that included tet(O) and a putative aminoglycoside transferase gene (aphA-3) highly similar to kanamycin resistance determinants. Several genes putatively involved in conjugative transfer were also identified on the plasmid. These findings will contribute to a better understanding of the distribution of potentially self-mobilizing plasmids harboring antibiotic resistance determinants in Campylobacter spp. from turkeys and other sources.
Source: Plasmid - Category: Biotechnology Source Type: research