Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis RSS feedThis is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Simple sequence repeat variations expedite phage divergence: mechanisms of indels and gene mutations
This study provides the first evidence of SSR variations expediting phage divergence, and enables insights into the events and mechanisms of genome evolution. NRUBME allows us to emulate natural evolution to design indels for various applications. Graphical abstract (Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis)
Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis - April 17, 2016 Category: Cytology Source Type: research

Charged Particle Mutagenesis at Low Dose and Fluence in Mouse Splenic T Cells
Publication date: Available online 29 March 2016 Source:Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis Author(s): Dmytro Grygoryev, Stacey Gauny, Michael Lasarev, Anna Ohlrich, Amy Kronenberg, Mitchell S. Turker High-energy heavy charged particles (HZE ions) found in the deep space environment can significantly affect human health by inducing mutations and related cancers. To better understand the relation between HZE ion exposure and somatic mutation, we examined cell survival fraction, Aprt mutant frequencies, and the types of mutations detected for mouse splenic T cells exposed in ...
Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis - March 29, 2016 Category: Cytology Source Type: research

Vincristine-induced bystander effect in human lymphocytes
In this study, we aimed to assess whether or not a bystander response can be induced in cultured human peripheral lymphocytes by vincristine, a chemotherapeutic mutagen acting as spindle poison, and by mitomycin-C, an alkylating agent already known to induce this response in human lymphoblastoid cells. Designing a modified ad hoc protocol for the cytokinesis blocked micronucleus (MN) assay, we detected the presence of a dose-dependent bystander response in untreated cultures receiving the conditioned medium (CM) from mitomycin-C (MMC) or vincristine (VCR) treated cultures. In the case of MMC, MN frequencies, expressed as m...
Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis - March 25, 2016 Category: Cytology Source Type: research

Lack of increased DNA double-strand breaks in peripheral blood mononuclear cells of individuals from high level natural radiation areas of Kerala coast in India
Publication date: Available online 25 March 2016 Source:Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis Author(s): Vinay Jain, P.R.Vivek Kumar, P.K.M. Koya, G. Jaikrishan, Birajalaxmi Das The high level natural radiation area (HLNRA) of Kerala is a 55km long and 0.5km wide strip in south west coast of India. The level of background radiation in this area varies from<1.0mGy/year to 45.0mGy/year. It offers unique opportunity to study the effect of chronic low dose/low dose-rate radiation directly on human population. Spontaneous level of DNA double strand breaks (DSBs) was quantifie...
Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis - March 25, 2016 Category: Cytology Source Type: research

Implications of genotypic differences in the generation of a urinary metabolomics radiation signature.
Publication date: Available online 25 March 2016 Source:Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis Author(s): Evagelia C. Laiakis, Evan L. Pannkuk, Maria Elena Diaz-Rubio, Yi-Wen Wang, Tytus D. Mak, Cynthia M. Simbulan-Rosenthal, David J. Brenner, Albert J. Fornace The increased threat of radiological terrorism and accidental nuclear exposures, together with increased usage of radiation-based medical procedures, has made necessary the development of minimally invasive methods for rapid identification of exposed individuals. Genetically predisposed radiosensitive individuals co...
Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis - March 25, 2016 Category: Cytology Source Type: research

Moderate malnutrition in rats induces somatic gene mutations
Publication date: Available online 8 March 2016 Source:Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis Author(s): M. Monserrat Pacheco-Martínez, Edith Cortés-Barberena, Elsa Cervantes-Ríos, María del Carmen García-Rodríguez, Leonor Rodríguez-Cruz, Rocío Ortiz-Muñiz The relationship between malnutrition and genetic damage has been widely studied in human and animal models, leading to the observation that interactions between genotoxic exposure and micronutrient status appear to affect genomic stability. A new assay has been developed that uses the phosphatidylinositol glycan cl...
Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis - March 18, 2016 Category: Cytology Source Type: research

Nucleotide excision repair deficiency increases levels of acrolein-derived cyclic DNA adduct and sensitizes cells to apoptosis induced by docosahexaenoic acid and acrolein
Publication date: Available online 16 March 2016 Source:Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis Author(s): Jishen Pan, Elizabeth Sinclair, Zhuoli Xuan, Marcin Dyba, Ying Fu, Supti Sen, Deborah Berry, Karen Creswell, Jiaxi Hu, Rabindra Roy, Fung-Lung Chung The acrolein derived cyclic 1,N2-propanodeoxyguanosine adduct (Acr-dG), formed primarily from ω-3 polyunsaturated fatty acids such as docosahexaenoic acid (DHA) under oxidative conditions, while proven to be mutagenic, is potentially involved in DHA-induced apoptosis. The latter may contribute to the chemopreventive...
Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis - March 16, 2016 Category: Cytology Source Type: research

Gene Mutations in Rats with Moderate Malnutrition
Publication date: Available online 8 March 2016 Source:Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis Author(s): M.Monserrat Pacheco-Martínez, Edith Cortés-Barberena, Elsa Cervantes-Ríos, María del Carmen García-Rodríguez, Leonor Rodríguez-Cruz, Rocío Ortiz-Muñiz The relationship between malnutrition and genetic damage has been widely studied in human and animal models, leading to the observation that interactions between genotoxic exposure and micronutrient status appear to affect genomic stability. A new assay has been developed that uses the phosphatidylinositol glycan cla...
Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis - March 10, 2016 Category: Cytology Source Type: research

H2A/K pseudogene mutation may promote cell proliferation
Publication date: Available online 2 March 2016 Source:Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis Author(s): Jisheng Guo, Ruirui Jing, Xin Lv, Xiaoyue Wang, Junqiang Li, Lin Li, Cuiling Li, Daoguang Wang, Baibing Bi, Xinjun Chen, Jinghua Yang Little attention has been paid to the histone H2A/K pseudogene. Results from our laboratory showed that 7 of 10 kidney cancer patients carried a mutant H2A/K pseudogene; therefore, we were interested in determining the relationship between mutant H2A/K and cell proliferation. We used shotgun and label-free proteomics methods to stud...
Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis - March 2, 2016 Category: Cytology Source Type: research

Radiation-induced changes in DNA methylation of repetitive elements in the mouse heart
In this study, we exposed C57BL/6J male mice to low absorbed mean doses of two types of space radiation − proton (0.1Gy, 150 MeV, dose rate 0.53±0.08Gy/min), and heavy iron ions (56Fe) (0.5Gy, 600 MeV/n, dose rate 0.38±0.06Gy/min). Radiation-induced changes in cardiac DNA methylation associated with repetitive elements were detected. Specifically, modest hypomethylation of retrotransposon LINE-1 was observed at day 7 after irradiation with either protons or 56Fe. This was followed by LINE-1, and other retrotransposons, ERV2 and SINE B1, as well as major satellite DNA hypermethylation at day 90 after irradiation with 56...
Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis - March 2, 2016 Category: Cytology Source Type: research

Increased MTHFR promoter methylation in mothers of Down syndrome individuals
Publication date: Available online 22 February 2016 Source:Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis Author(s): Fabio Coppedè, Maria Denaro, Pierpaola Tannorella, Lucia Migliore Despite that advanced maternal age at conception represents the major risk factor for the birth of a child with Down syndrome (DS), most of DS babies are born from women aging less than 35 years. Studies performed in peripheral lymphocytes of those women revealed several markers of global genome instability, including an increased frequency of micronuclei, shorter telomeres and impaired global DNA methylatio...
Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis - February 23, 2016 Category: Cytology Source Type: research

Differences in the Origins of Kinetochore-positive and Kinetochore-negative Micronuclei: A Live Cell Imaging Study
Publication date: Available online 23 February 2016 Source:Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis Author(s): Erkang Jiang Micronuclei (MNi) are extensively used to evaluate genotoxicity and chromosomal instability. Classification of kinetochore-negative (K-MNi) and kinetochore-positive micronuclei (K+MNi) improves the specificity and sensitivity of the micronucleus (MN) test; however, the fundamental differences in the origins of K-MNi and K+MNi have not been addressed due to the limitations of traditional methods. In the current study, HeLa CENP B-GFP H2B-mCherry cells were constructed...
Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis - February 23, 2016 Category: Cytology Source Type: research

Identification of six novel mutations in Iranian patients with maple syrup urine disease and their in silico analysis​
In this study, two sets of multiplex polymorphic STR (Short Tandem Repeat) markers linked to the above genes were used to aid in homozygosity mapping in order to find probable pathogenic change(s) in the studied families. The families who showed homozygote haplotype for the BCKDHA gene were subsequently sequenced. Our findings showed that exons 2, 4 and 6 contain most of the mutations which are novel. The changes include two single nucleotide deletion (i.e. c. 143delT and c.702delT), one gross deletion covering the whole exon four c.(375+1_376-1)_(8849+1_885-1), two splice site changes (c.1167+1G>T, c. 288+1G&gt...
Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis - February 21, 2016 Category: Cytology Source Type: research

TRAIL causes deletions at the HPRT and TK1 loci of clonogenically competent cells
In this study we compared the types of mutations sustained in the HPRT and TK1 loci of clonogenically competent cells following treatment with TRAIL or the alkylating agent ethyl methanesulfonate (EMS). As expected, the loss-of-function mutations in the HPRT or TK1 loci triggered by exposure to EMS were almost all transitions. In contrast, only a minority of the mutations identified in TRAIL-treated clones lacking HPRT or TK1 activity were substitutions. Almost three quarters of the TRAIL-induced mutations were partial or complete deletions of the HPRT or TK1 genes, consistent with sub-lethal TRAIL treatment provoking doub...
Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis - February 20, 2016 Category: Cytology Source Type: research

FANCJ at the FORK
Publication date: Available online 17 February 2016 Source:Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis Author(s): Sharon B. Cantor, Sumeet Nayak (Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis)
Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis - February 18, 2016 Category: Cytology Source Type: research