Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

A Novel Type of Gene Interaction in D. melanogaster
Publication date: Available online 11 January 2017 Source:Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis Author(s): B.F. Chadov, E.V. Chadova, N.B. Fedorova The genes interact according to classical mechanisms, namely, complementation, modification, polymery, and epistasis, in the cells and organisms carrying these genes. Here we describe a novel type of gene interaction when the interacting genes reside in parents, whereas the interaction event takes place in their progenies lacking these genes. The conditional mutations in the D. melanogaster male X chromosome caused the “prohibition on prod...
Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis - January 10, 2017 Category: Cytology Source Type: research

The DNA damage response of C. elegans affected by gravity sensing and radiosensitivity during the Shenzhou-8 spaceflight
Publication date: Available online 7 January 2017 Source:Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis Author(s): Ying Gao, Dan Xu, Lei Zhao, Yeqing Sun Space radiation and microgravity are recognized as primary and inevitable risk factors for humans traveling in space, but the reports regarding their synergistic effects remain inconclusive and vary across studies due to differences in the environmental conditions and intrinsic biological sensitivity. Thus, we studied the synergistic effects on transcriptional changes in the global genome and DNA damage response (DDR) by using dys-1 mutant and...
Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis - January 6, 2017 Category: Cytology Source Type: research

Synergism of dam, MutH, and MutS in methylation-directed mismatch repair in Escherichia coli
This study showed that mutS defect produced a higher mutation frequency than mutH did. Interestingly, double defects in dam and mutS synergistically produced a dramatically higher spontaneous mutation frequency than the summation of mutation frequencies of FX-11 strains with individual deficiency of dam or mutS, suggesting that Dam may work with MutHL to partially accomplish the task of recognizing the mismatch sites to retain partial mismatch repair capacity. (Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis)
Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis - January 5, 2017 Category: Cytology Source Type: research

Sample size determination for the fluctuation experiment
Publication date: Available online 19 December 2016 Source:Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis Author(s): Qi Zheng The Luria-Delbrück fluctuation experiment protocol is increasingly employed to determine microbial mutation rates in the laboratory. An important question raised at the planning stage is “How many cultures are needed?” For over 70 years sample sizes have been determined either by intuition or by following published examples where sample sizes were chosen intuitively. This paper proposes a practical method for determining the sample size. The proposed method relies on e...
Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis - December 19, 2016 Category: Cytology Source Type: research

Influence of reduced glutathione on end-joining of DNA double-strand breaks: Cytogenetical and molecular approach
Publication date: Available online 9 November 2016 Source:Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis Author(s): Nitin Ghoshal, Sheetal Sharma, Atanu Banerjee, Sillarine Kurkalang, Sathees C Raghavan, Anupam Chatterjee Radiation induced DNA double-strand breaks (DSB) are the major initial lesions whose misrejoining may lead to exchange aberrations. However, the role of glutathione (GSH), a major cellular thiol, in regulating cell’s sensitivity to DNA damaging agents is not well understood. Influence of endogenous GSH on the efficiency of X-rays and bleomycin (Blem) induced DNA DSBs end-j...
Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis - November 9, 2016 Category: Cytology Source Type: research

Effects of Ru(CO)3Cl-glycinate on the Developmental Toxicities induced by X-ray and Carbon-ion Irradiation in Zebrafish Embryos
Publication date: Available online 5 November 2016 Source:Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis Author(s): Rong Zhou, Jing’e Song, Jing Si, Hong Zhang, Bin Liu, Lu Gan, Xin Zhou, Yupei Wang, Junfang Yan, Qianjing Zhang The inhibitory effects of carbon monoxide (CO), generated by Ru(CO)3Cl-glycinate [CO-releasing molecule (CORM-3)], on developmental toxicity in zebrafish embryos induced by ionizing radiation with different linear energy transfer (LET) were studied. Zebrafish embryos at 5h post-fertilization were irradiated with X-ray (low-LET) and carbon-ion (high-LET) with or w...
Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis - November 5, 2016 Category: Cytology Source Type: research

Mapping the factors affecting the frequency and types of micronuclei in an elderly population from Southern Bohemia
Publication date: Available online 26 October 2016 Source:Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis Author(s): Andrea Rossnerova, Katerina Honkova, Jitka Pavlikova, Zuzana Freitinger Skalicka, Renata Havrankova, Ivo Solansky, Pavel Rossnerr, Radim J. Sram, Friedo Zölzer The micronucleus assay is one of the most common methods used to assess chromosomal damage (losses or breaks) in human peripheral blood lymphocytes (PBL) in genetic toxicology. Most studies have focused on analyzing total micronuclei (MN), but identifying the content of MN can provide more detailed information. The ma...
Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis - October 28, 2016 Category: Cytology Source Type: research

Transposon-mediated activation of the Escherichia coli glpFK operon is inhibited by specific DNA-binding proteins: Implications for stress-induced transposition events
Publication date: November–December 2016 Source:Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, Volumes 793–794 Author(s): Zhongge Zhang, Milton H. Saier Escherichia coli cells deleted for the cyclic AMP (cAMP) receptor protein (Crp) gene (Δcrp) cannot utilize glycerol because cAMP-Crp is a required activator of the glycerol utilization operon, glpFK. We have previously shown that a transposon, Insertion Sequence 5 (IS5), can insert into the upstream regulatory region of the operon to activate the glpFK promoter and enable glycerol utilization. GlpR, which represses glpFK transcription, bind...
Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis - October 28, 2016 Category: Cytology Source Type: research

Evidence for cell-free nucleic acids as continuously arising endogenous DNA mutagens
This study suggests that circulating extracellular cfNAs act as physiological continuously arising DNA mutagens with implications for ageing, cancer and a host of other degenerative human pathologies. (Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis)
Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis - October 28, 2016 Category: Cytology Source Type: research

Cell-free nucleic acids as continuously arising endogenous DNA mutagens: A new proposal
This study suggests that circulating extracellular cfNAs act as physiological continuously arising DNA mutagens with implications for ageing, cancer and a host of other degenerative human pathologies. (Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis)
Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis - October 13, 2016 Category: Cytology Source Type: research

Screen for Genes Involved in Radiation Survival of Escherichia coli and Construction of a Reference database
Publication date: Available online 3 October 2016 Source:Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis Author(s): Neil Sargentini, Nicholas P. Gularte, Deborah A. Hudman A set of 3,907 single-gene knockout (Keio collection) strains of Escherichia coli K-12 was examined for strains with increased susceptibility to killing by X- or UV-radiation. After screening with a high-throughput resazurin-based assay and determining radiation survival with triplicate clonogenic assays, we identified 76 strains (and associated deleted genes) showing statistically-significant increased radiation sensitivity co...
Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis - October 2, 2016 Category: Cytology Source Type: research

Occupational exposure to anesthetics leads to genomic instability, cytotoxicity and proliferative changes
In conclusion, exposure to modern waste anesthetic gases did not induce systemic DNA damage, but it did result in genomic instability, cytotoxicity and proliferative changes, which were detected in the EBC of anesthesiologists. Thus, these professionals can be considered at risk for developing genetic alterations resulting from occupational exposure to these gases, suggesting the need to minimize this exposure. (Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis)
Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis - September 12, 2016 Category: Cytology Source Type: research

Human circulating ribosomal dna content significantly increases while circulating satellite iii (1q12) content decreases under chronic occupational exposure to low-dose gamma- neutron and tritium Βeta-radiation
Publication date: Available online 7 September 2016 Source:Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis Author(s): Inna B. Korzeneva, Svetlana V. Kostuyk, Elizaveta S. Ershova, Elena N. Skorodumova, Veronika F. Zhuravleva, Galina V. Pankratova, Irina V. Volkova, Elena V. Stepanova, Lev N. Porokhovnik, Natalia N. Veiko A single exposure to ionizing radiation (IR) results in an elevated cell-free DNA (cfDNA) content in the blood plasma. In this case, the cfDNA concentration can be a marker of the cell death in the organism. However, a chronic exposure to a low-dose IR enhances both the en...
Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis - September 6, 2016 Category: Cytology Source Type: research

The role of DNA polymerase ζ in translesion synthesis across bulky DNA adducts and cross-links in human cells
Publication date: Available online 25 August 2016 Source:Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis Author(s): Tetsuya Suzuki, Petr Grúz, Masamitsu Honma, Noritaka Adachi, Takehiko Nohmi Translesion DNA synthesis (TLS) is a cellular defense mechanisms against genotoxins. Defects or mutations in specialized DNA polymerases (Pols) involved in TLS are believed to result in hypersensitivity to various genotoxic stresses. Here, DNA polymerase ζ (Pol ζ)-deficient (KO: knockout) and Pol ζ catalytically dead (CD) human cells were established and their sensitivity towards cytotoxic activities o...
Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis - August 24, 2016 Category: Cytology Source Type: research

Influence of exposure to pesticides on telomere length in tobacco farmers: a biology system approach
Publication date: Available online 17 August 2016 Source:Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis Author(s): Vivian Francília Silva Kahl, Juliana da Silva, Fernanda Rabaioli da Silva Various pesticides in the form of mixtures must be used to keep tobacco crops pest-free. Recent studies have shown a link between occupational exposure to pesticides in tobacco crops and increased damage to the DNA, mononuclei, nuclear buds and binucleated cells in buccal cells as well as micronuclei in lymphocytes. Furthermore, pesticides used specifically for tobacco crops shorten telomere length (TL) signi...
Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis - August 17, 2016 Category: Cytology Source Type: research