Rapid and visual detection of dengue virus using recombinase polymerase amplification method combined with lateral flow dipstick
Publication date: Available online 14 June 2019Source: Molecular and Cellular ProbesAuthor(s): Yun Xi, Chang-Zhi Xu, Zhi-Zhi Xie, Dong-Lin Zhu, Jie-Ming DongAbstractDengue virus (DENV), a member of the genus Flavivirus within the family Flaviviridae, is one of the most significant mosquito-borne viruses that causing dengue fever in human. A rapid diagnostic would be helpful to detect DENV infection in a timely manner. In the last decade, recombinase polymerase amplification (RPA) technique has been experiencing rapid development and widely employed to detect various other pathogens. In present study, a reverse transcriptio...
Source: Molecular and Cellular Probes - June 14, 2019 Category: Molecular Biology Source Type: research
A PCR-based NGS protocol for whole genome sequencing of West Nile virus lineage 2 directly from biological specimens
Publication date: Available online 12 June 2019Source: Molecular and Cellular ProbesAuthor(s): Serafeim C. Chaintoutis, Elpida Papadopoulou, Angeliki Melidou, Anna Papa, Chrysostomos I. DovasAbstractLineage 2 West Nile virus (WNV) strains have been implicated in severe encephalitis outbreaks in humans and equines residing in Europe. WNV molecular characterization is important for the development of diagnostic assays, as well as for obtaining molecular information, which is necessary for epidemiological investigations of virus transmission in areas at risk. For whole genome sequencing of lineage 2 WNV strains, directly from...
Source: Molecular and Cellular Probes - June 13, 2019 Category: Molecular Biology Source Type: research
Assessment of ctDNA in CSF may be a more rapid means of assessing surgical outcomes than plasma ctDNA in glioblastoma
Publication date: Available online 4 June 2019Source: Molecular and Cellular ProbesAuthor(s): Jue-hui Li, Zhen-qiang He, Fu-hua Lin, Zheng-he Chen, Shi-yu Yang, Hao Duan, Xiao-bing Jiang, Fuad Al-Nahari, Xiang-heng Zhang, Jiang-huang Wang, Guan-hua Zhang, Zhen-feng Zhang, Cong Li, Yong-Gao MouAbstractWe aimed to develop a high-throughput deep DNA sequencing assay of cerebrospinal fluid (CSF) to identify clinically relevant oncogenic mutations that contribute to the development of glioblastoma (GBM) and serve as biomarkers to predict patients' responses to surgery. For this purpose, we recruited five patients diagnosed with...
Source: Molecular and Cellular Probes - June 4, 2019 Category: Molecular Biology Source Type: research
Editorial Board
Publication date: June 2019Source: Molecular and Cellular Probes, Volume 45Author(s): (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - May 28, 2019 Category: Molecular Biology Source Type: research
Recovery of Muscovy duck-origin goose parvovirus from an infectious clone containing an E-box motif (CACATG) deletion within the left terminal region
This study laid the foundation for further understanding of the relationship between E-box deletion in the L-ITR and MDGPV virulence. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - May 24, 2019 Category: Molecular Biology Source Type: research
SI: Next-generation sequencing in human molecular genetic diagnostics
Publication date: Available online 15 May 2019Source: Molecular and Cellular ProbesAuthor(s): Sabine Hoffjan (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - May 15, 2019 Category: Molecular Biology Source Type: research
Endostar regulates EMT, migration and invasion of lung cancer cells through the HGF-Met pathway
ConclusionES suppresses lung cancer progression by down-regulating HGF-Met signaling, revealing the possible mechanism of ES in the process of treating lung cancer patients. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - May 14, 2019 Category: Molecular Biology Source Type: research
In silico prediction of B-cell epitopes for twenty-five mite allergens: The therapeutic potentials for immunotherapy
Publication date: Available online 14 May 2019Source: Molecular and Cellular ProbesAuthor(s): Narjes Ebrahimi, Navid Nezafat, Hossein Esmaeilzadeh, Younes Ghasemi, Seyed Hesamodin Nabavizadeh, Soheila AlyasinAbstractMite allergens are one of the major allergens; however, their structures and epitopes have not been thoroughly studied. In the present study, we predicted the tertiary structures of several mite allergens and also identified the B-cell epitopes, which can be suggested as potential epitopes for allergen immunotherapy. Twenty-five mite allergens, from six mite allergen groups, were investigated; homology modeling...
Source: Molecular and Cellular Probes - May 14, 2019 Category: Molecular Biology Source Type: research
Development of a loop-mediated isothermal amplification assay for Vibrio cholerae O1 and O139
Publication date: Available online 10 May 2019Source: Molecular and Cellular ProbesAuthor(s): Hidemasa Izumiya, Masatomo Morita, Eiji Arakawa, Tuan Cuong Ngo, Hoai Thu Nguyen, Dong Tu Nguyen, Makoto OhnishiAbstractA loop-mediated isothermal amplification assay was developed. It was designed for recognizing Vibrio cholerae O1/O139, where atpA, rfbN, and wfbR genes were adopted. The assay specifically detected the target with sensitivities of 5–67 copies per reaction in 1 h. The assay will aid rapid detection of the cholera bacterium. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - May 11, 2019 Category: Molecular Biology Source Type: research
Let-7 inhibits the migration and invasion of extravillous trophoblast cell via targeting MDM4
ConclusionsLet-7 silencing promoted proliferation, migration and invasion in EVT cells through the up-regulation of MDM4. Our study provided new insights into the molecular mechanism of PE. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - May 10, 2019 Category: Molecular Biology Source Type: research
NGS-Based genetic testing for heritable cardiovascular diseases. Specific requirements for obtaining informed consent
Publication date: Available online 3 May 2019Source: Molecular and Cellular ProbesAuthor(s): Jörg Schmidtke, Kathrin Wittkowski, Ralf GlaubitzAbstractClinical genetic testing in cardiovascular genetic medicine has undergone rapid changes. Next generation sequencing allows simultaneous testing of all genes associated with any cardiovascular phenotype, and molecular genetic testing for multiple genes has become the standard of practice for cardiovascular medicine. While technical and clinical advantages of multigenic approaches are evident, informed consent procedures have become more complex and challenging to the physicia...
Source: Molecular and Cellular Probes - May 4, 2019 Category: Molecular Biology Source Type: research
Association among ACE, ESR1 polymorphisms and preeclampsia in Brazilian pregnant women
ConclusionThis pioneer study involving Brazilian women showed no association among the studied polymorphisms and PE, which suggests that ins/del ACE and SNPs ESR1 do not contribute to this disease occurrence in Brazil. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - April 25, 2019 Category: Molecular Biology Source Type: research
A TaqMan probe-based real-time PCR to differentiate porcine epidemic diarrhea virus virulent strains from attenuated vaccine strains
Publication date: Available online 17 April 2019Source: Molecular and Cellular ProbesAuthor(s): Jing Liu, Li-min Li, Jiu-qaun Han, Tai-ran Sun, Xue Zhao, Rui-tao Xu, Qin-ye SongAbstractPorcine epidemic diarrhea virus (PEDV) is an important pathogen causing severe watery diarrhea, vomiting, dehydration, and death in sucking piglets. Attenuated vaccines have been used widely in sows in order to protect piglets through passive lactogenic immunity. Rapid and sensitive detection methods for differentiating attenuated vaccine strains from virulent ones are essential and practical in PEDV prevention and control. Based on the dele...
Source: Molecular and Cellular Probes - April 18, 2019 Category: Molecular Biology Source Type: research
An exonuclease-assisted fluorescence sensor for assaying alkaline phosphatase based on SYBR Green I
In this report, we propose a fast, reliable and convenient approach to determine the alkaline phosphatase (ALP) activity based on a label-free fluorescence strategy. Upon catalysis of ALP, dephosphorylated dsDNA hampers the λ exonuclease (λexo) cleavage, shows high affinity to SYBR Green I (SG I), resulting in a strong fluorescence emission peak at 520 nm. In the absence of ALP, the dsDNA with 5′-phosphoryl-termini could be employed as a substrate of λexo. After cleavage, a weak fluorescence emission peaks at 520 nm could be observed. The assay was both selective and sensitive, and the detection limit was found to...
Source: Molecular and Cellular Probes - April 13, 2019 Category: Molecular Biology Source Type: research
Development of a TB green II-based duplex real-time fluorescence quantitative PCR assay for the simultaneous detection of porcine circovirus 2 and 3
Publication date: Available online 10 April 2019Source: Molecular and Cellular ProbesAuthor(s): Yu Zhao, Hao-ying Han, Lin Fan, Run-Bo Tian, Jian-Tao Cui, Jin-Yi Li, Hong-Ying Chen, Ming-Fan Yang, Lan-Lan ZhengAbstractPorcine circovirus 3 (PCV3), as a newly emerged circovirus, is widely distributed in pig populations worldwide. Co-infection of PCV2 and PCV3 has been reported frequently in clinical samples. In the present study, a TB Green II-based duplex real-time polymerase chain reaction (qPCR) was developed to rapidly and differentially detect PCV2 and PCV3. The assay specifically detected PCV2 and PCV3, with no fluores...
Source: Molecular and Cellular Probes - April 11, 2019 Category: Molecular Biology Source Type: research
