Downregulated low-density lipoprotein receptor-related protein 6 induces the maldevelopment of extravillous trophoblast via Wnt/β-catenin signaling pathway
Publication date: Available online 24 January 2019Source: Molecular and Cellular ProbesAuthor(s): Xiaoyan Yu, Yan Zhang, Ping Yang, Xueli Gao, Yuping WangAbstractPreeclampsia (PE), a special type of hypertensive disorder complicating pregnancy (HDCP), is highly associated with the migratory and invasive capacity of extravillous trophoblasts (EVTs). Here, we aimed to study the functions of low-density lipoprotein receptor-related protein 6 (LRP6) in PE pathogenesis. A comparative analysis of cellular gene expressions between placenta tissues collected from PE patients and normal pregnant women showed that the expressions of...
Source: Molecular and Cellular Probes - January 24, 2019 Category: Molecular Biology Source Type: research
HADHA and HADHB gene associated phenotypes - Identification of rare variants in a patient cohort by Next Generation Sequencing
Publication date: Available online 22 January 2019Source: Molecular and Cellular ProbesAuthor(s): Isabel Diebold, Ulrike Schön, Rita Horvath, Oliver Schwartz, Elke Holinski-Feder, Heike Kölbel, Angela AbichtAbstractThe heterooctameric mitochondrial trifunctional protein (MTP), composed of four α- and β-subunits harbours three enzymes that each perform a different function in mitochondrial fatty acid β-oxidation. Pathogenic variants in the MTP genes (HADHA and HADHB) cause MTP deficiency, a rare autosomal recessive metabolic disorder characterized by phenotypic heterogeneity ranging from severe, early-onset, cardiac di...
Source: Molecular and Cellular Probes - January 23, 2019 Category: Molecular Biology Source Type: research
Editorial Board
Publication date: February 2019Source: Molecular and Cellular Probes, Volume 43Author(s): (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - January 20, 2019 Category: Molecular Biology Source Type: research
Novel dual-color drug screening model for GLUT4 translocation in adipocytes
In conclusion, we developed an easy-to-apply dual color GLUT4 probe to monitor GLUT4 translocation in insulin-responsive cells, which could be alternatively employed to high-throughput screen compounds regulating GLUT4 translocation and glucose uptake, even to dissect GLTU4 approaching, docking and fusion with the plasma membrane (PM), and to reveal relevant molecular mechanisms involved in these steps as expected. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - January 12, 2019 Category: Molecular Biology Source Type: research
A real-time recombinase polymerase amplification assay for the rapid detection of Vibrio harveyi
Publication date: Available online 3 January 2019Source: Molecular and Cellular ProbesAuthor(s): Jianhu Pang, Qiong Wang, Yuejun Fei, Peng Zhu, Longliang Qiao, Hailong Huang, Chenyang Dang, Weifang GaoAbstractVibrio harveyi is a pathogen that infects fish and shellfish worldwide, causing severe economic losses for the aquaculture industry. As the early diagnosis of V. harveyi infection is crucial to disease surveillance and prevention in cultured marine animals, a fast and accurate method to detect V. harveyi is required. Here, we performed recombinase polymerase amplification (RPA) using novel primers specifically designe...
Source: Molecular and Cellular Probes - January 3, 2019 Category: Molecular Biology Source Type: research
Next generation sequencing and imprinting disorders: Current applications and future perspectives: Lessons from Silver-Russell syndrome
Publication date: Available online 2 January 2019Source: Molecular and Cellular ProbesAuthor(s): Lea Neuheuser, Robert Meyer, Matthias Begemann, Miriam Elbracht, Thomas EggermannAbstractImprinting Disorders are a group of rare diseases with overlapping phenotypes which are associated with similar molecular changes and affect imprinted chromosomal regions. Clinical features mainly occur prenatally or in childhood, but have a severe lifelong impact on health. Due to their clinical and molecular heterogeneity, the diagnosis of imprinting disorders is often challenging and requires testing of a broad spectrum of genomic varian...
Source: Molecular and Cellular Probes - January 2, 2019 Category: Molecular Biology Source Type: research
Direct comparison of QIAamp DSP Virus Kit and QIAamp Circulating Nucleic Acid Kit regarding cell-free fetal DNA isolation from maternal peripheral blood
ConclusionsWe recommend CNAK over DSPVK for quantitative analysis of cffDNA. Nevertheless, DSPVK is definitely suitable for qualitative analysis as well as for research with limited budget, since it is almost 3 times cheaper than CNAK. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - December 23, 2018 Category: Molecular Biology Source Type: research
Rapid detection of Cucumber green mottle mosaic virus by reverse transcription recombinase polymerase amplification
Publication date: Available online 22 December 2018Source: Molecular and Cellular ProbesAuthor(s): Rong Zeng, Jinyan Luo, Shigang Gao, Lihui Xu, Zhiwei Song, Fuming DaiAbstractReverse transcription recombinase polymerase amplification (RT-RPA) for detection of Cucumber green mottle mosaic virus (CGMMV) was developed in this study. It was found to be specific, with a limit of detection of 0.5 pg of total CGMMV RNA. CGMMV on inoculated leaves was tested using RT-RPA assay, suggesting that this method is suitable for CGMMV detection from leaves. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - December 22, 2018 Category: Molecular Biology Source Type: research
Multiplex SYBR Green and duplex TaqMan real-time PCR assays for the detection of Photorhabdus Insect-Related (Pir) toxin genes pirA and pirB
Publication date: Available online 18 December 2018Source: Molecular and Cellular ProbesAuthor(s): Roberto Cruz-Flores, Hung Nam Mai, Arun K. DharAbstractAcute hepatopancreatic necrosis disease (AHPND), also known as Early mortality syndrome (EMS), is a recently emerged lethal disease that has caused major economic losses in shrimp aquaculture. The etiologic agents are Vibrio spp. that carry Photorhabdus Insect-Related (Pir) toxin genes pirA and pirB. A multiplex SYBR Green real-time PCR was developed that detects pirA, pirB, and two internal control genes, the shrimp 18S rRNA and the bacterial 16S rRNA genes in a single r...
Source: Molecular and Cellular Probes - December 19, 2018 Category: Molecular Biology Source Type: research
A novel fluorometric method for inorganic pyrophosphatase detection based on G-quadruplex-thioflavin T
Publication date: Available online 17 December 2018Source: Molecular and Cellular ProbesAuthor(s): Han Zhao, Changbei Ma, Mingjian ChenAbstractIn this paper, we propose a fluorometric approach for the highly sensitive detection of inorganic pyrophosphatase (PPase) based on G-quadruplex-thioflavin T (ThT). In the absence of PPase, Cu2+ can coordinate with pyrophosphate (PPi) to generate a Cu2+/PPi complex. Then the G-rich sequence folds into the G-quadruplex structure, which can combine with ThT to generate a remarkable fluorescent signal. In the presence of PPase, the coordinated compound can be destroyed by the PPase cata...
Source: Molecular and Cellular Probes - December 18, 2018 Category: Molecular Biology Source Type: research
Application of random amplified polymorphism DNA and 16S-23S rDNA intergenic spacer polymerase chain reaction-restriction fragment length polymorphism to predict major Streptococcus suis clonal complexes isolated from humans and pigs
This study revealed that RAPD had a greater discriminatory power than PCR-RFLP. This assay will be useful for screening or predicting major CCs relevant to human and pig S. suis clinical isolates and for low-cost screening of large numbers of isolates with rapid analytical capacity and could be utilized in most laboratories. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - December 6, 2018 Category: Molecular Biology Source Type: research
Ginsenoside Rb1 and mitochondria: A short review of the literature
Publication date: Available online 4 December 2018Source: Molecular and Cellular ProbesAuthor(s): Ping Zhou, Weijie Xie, Yifan Sun, Ziru Dai, Guang Li, Guibo Sun, Xiaobo SunAbstractMitochondria play a central role in various critical cellular processes, including energy synthesis, energy supply and apoptosis. Panax notoginseng, a commonly used traditional Chinese medicine, has various pharmacological effects on the human body. Ginsenosides are representative bioactive components of P. notoginseng. Recently, more attention has focused on ginsenoside Rb1 as an antioxidative and anti-inflammatory agent that can protect the ne...
Source: Molecular and Cellular Probes - December 5, 2018 Category: Molecular Biology Source Type: research
Prediction of epitopes of Neisseria Gonorrhoeae against USA human leukocyte antigen background: An immunoinformatic approach towards development of future vaccines for USA population
Publication date: Available online 29 November 2018Source: Molecular and Cellular ProbesAuthor(s): Malik Siddique Mahmood, Muhammad Asad Ullah, Hina Batool, Sana Batool, Naeem Mehmood AshrafAbstractGonorrheal infections are the second most prevalent sexually transmitted diseases STDs in the USA populations after Chlamydia. These infections have now become an urgent problem to address because Neisseria gonorrhoeae is capable of developing resistance to multiple antibiotic classes. Infection with these antibiotic-resistant strains has become the major public health concern. Although extensive researches are ongoing to contro...
Source: Molecular and Cellular Probes - November 29, 2018 Category: Molecular Biology Source Type: research
Selective growth inhibition of Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans by antisense peptide nucleic acids
Publication date: Available online 22 November 2018Source: Molecular and Cellular ProbesAuthor(s): Sadaomi Sugimoto, Hiroshi Maeda, Mizuki Kitamatsu, Ikuo Nishikawa, Muneyasu ShidaAbstractPeptide nucleic acids (PNA) are DNA/RNA analogs in which the sugar-phosphate backbone is replaced by N-2-aminoethylglycine. PNA are widely used for experimental antisense therapy due to their strong affinity to mRNA. By targeting specific genes, protein synthesis and the growth of bacteria or cancer cells can be inhibited by PNA. Here, we report the design and evaluation of antisense PNA for selective growth inhibition of Porphyromonas gi...
Source: Molecular and Cellular Probes - November 22, 2018 Category: Molecular Biology Source Type: research
Development of a droplet digital PCR assay for sensitive detection of porcine circovirus 3
In this study, we developed a novel droplet digital PCR (ddPCR) assay targeting the PCV3 cap gene to improve the sensitivity of PCV3 detection. The established assay is highly specific to PCV3, and does not cross react with other important swine pathogens. The assay's detection limit was 1.68 ± 0.29 copies of PCV3 DNA per reaction (n = 8), an approximately 10-fold greater sensitivity than that of our previously developed quantitative real-time PCR (qPCR) assay for the same virus. The ddPCR assay results were highly reproducible, with intra- and inter-assay coefficient of variation values of <9.0%. Of the 239 a...
Source: Molecular and Cellular Probes - November 21, 2018 Category: Molecular Biology Source Type: research
