Identification of FimH derivatives as adjuvant vaccinated with PAc that enhance protection against Streptcoccus mutans colonization
In this study, adjuvant potential of FimH variants was evaluated by investigating their ability to trigger innate immune response to DC2.4 and adaptive immunity to improve immunological characteristics. The data revealed that purified A106 and T158P up-regulated the expression of co-stimulatory molecules critically involved in DC2.4 activation by interaction with TLR4, whereas A25P and G73R did not induce the phenotypic maturation of DC2.4. Besides, the culture of DC2.4 with A106 and T158P enhanced the release of cytokines and protein phagocytosis. When formulated with PAc, T158P elicited more robust PAc-specific IgG and I...
Source: Molecular and Cellular Probes - March 31, 2019 Category: Molecular Biology Source Type: research
Fabry disease: Detection of Alu-mediated exon duplication by NGS
Publication date: Available online 29 March 2019Source: Molecular and Cellular ProbesAuthor(s): Martin Farr, Susana Ferreira, Arwa Al-Dilaimi, Sonja Bögeholz, Alexander Goesmann, Jörn Kalinowski, Cornelius Knabbe, Lothar Faber, João Paulo Oliveira, Volker RudolphAbstractMonogenetic diseases can be analyzed routinely by targeted DNA sequencing. If causative variants are not found, complementary methods like RNA sequencing or analysis of copy number variations by multiplex ligation-dependent probe amplification have to be considered. In the latter, especially exonic duplications or deletions can be detected, but the preci...
Source: Molecular and Cellular Probes - March 30, 2019 Category: Molecular Biology Source Type: research
Direct detection of Actinobacillus pleuropneumoniae in swine lungs and tonsils by real-time recombinase polymerase amplification assay
In this study, a real-time recombinase polymerase amplification assay (real-time RPA) based on the apxIVA gene was developed to rapid detect A. pleuropneumoniae. Real-time RPA was performed successfully in Genie III at the constant temperature of 39 °C for 20 min. The developed assay was highly specific for A. pleuropneumoniae, and the sensitivity at 95% probability was 536 fg of A. pleuropneumoniae genomic DNA. The real-time RPA for A. pleuropneumoniae was further evaluated on the 112 clinical swine lung and tonsil samples, and 25 (22.3%), 27 (24.1%), and 12 (10.7%) samples were positive for A. pleuropneumoniae by ...
Source: Molecular and Cellular Probes - March 29, 2019 Category: Molecular Biology Source Type: research
Corrigendum to “Enhanced multiplex PCR assay for the typing of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus”
Publication date: Available online 28 March 2019Source: Molecular and Cellular ProbesAuthor(s): Kunyan Zhang, Jo-Ann McClure, John M. Conly (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - March 28, 2019 Category: Molecular Biology Source Type: research
Development of a reverse transcription recombinase polymerase amplification assay for rapid detection of human respiratory syncytial virus
Publication date: Available online 25 March 2019Source: Molecular and Cellular ProbesAuthor(s): Yun Xi, Chang-Zhi Xu, Zhi-Zhi Xie, Dong-Lin Zhu, Jie-Ming Dong, Gang XiaoAbstractRespiratory syncytial virus (RSV) is one of the most important causative agents that causing respiratory tract infection in children and associated with high morbidity and mortality. A diagnostic method would be a robust tool for identification of RSV infection, especially in the resource-limited settings. Recombinase polymerase amplification (RPA) is a novel isothermal amplification technique which has been widely employed to detect human/animal pa...
Source: Molecular and Cellular Probes - March 26, 2019 Category: Molecular Biology Source Type: research
Which genes to assess in the NGS diagnostics of intellectual disability? The case for a consensus database-driven and expert-curated approach
Publication date: Available online 23 March 2019Source: Molecular and Cellular ProbesAuthor(s): Florian Erger, Christian P. Schaaf, Christian NetzerAbstractWhen deciding on which genes to assess in larger Next-Generation Sequencing (NGS) datasets for the molecular genetic diagnosis of intellectual disability (ID), geneticists today have a variety of gene-phenotype databases and expert-curated gene lists available. To quantify their respective completeness, we compare an ID gene selection auto-generated from the Human Phenotype Ontology gene-phenotype association database and expert-curated ID gene lists from three reputabl...
Source: Molecular and Cellular Probes - March 24, 2019 Category: Molecular Biology Source Type: research
Editorial Board
Publication date: April 2019Source: Molecular and Cellular Probes, Volume 44Author(s): (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - March 24, 2019 Category: Molecular Biology Source Type: research
Α 2-stage, nested-like nucleic acid amplification method (IsoPCR) for the highly sensitive detection of HPV16 and HPV18 DNA
In conclusion, IsoPCR is a highly sensitive and specific diagnostic tool and it is suitable for the detection of low copy number of viral DNA in clinical specimens, providing critical information to healthcare providers. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - March 21, 2019 Category: Molecular Biology Source Type: research
Use of whole exome sequencing in the NICU: Case of an extremely low birth weight infant with syndromic features
In conclusion, our case illustrated that CS should be considered in ELBW infants showing a life-threatening combination of complex cardiac arrhythmia and hypokinesia. If a syndromic disorder is suspected in the neonatal intensive care unit (NICU) setting, WES is a useful, non-invasive diagnostic tool in critically ill ELBW infants. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - March 15, 2019 Category: Molecular Biology Source Type: research
Probe-specific loop-mediated isothermal amplification magnetogenosensor assay for rapid and specific detection of pathogenic Leptospira
This study highlighted the performance of the developed integrated loop-mediated isothermal amplification (LAMP) coupled with a colorimetric DNA-based magnetogenosensor. The biosensor operates through a DNA hybridization system in which a specific designed probe captures the target LAMP amplicons. We demonstrated the magnetogenosensor assay by detecting pathogenic Leptospira, which causes leptospirosis. The color change of the assay from brown to blue indicated a positive result, whereas a negative result was indicated by the assay maintaining its brown color. The DNA biosensor was able to detect DNA at a concentration as ...
Source: Molecular and Cellular Probes - March 13, 2019 Category: Molecular Biology Source Type: research
CDKL1 promotes the chemoresistance of human oral squamous cell carcinoma cells to hydroxycamptothecin
Publication date: Available online 22 February 2019Source: Molecular and Cellular ProbesAuthor(s): Keyi Li, Zhen Meng, Licheng Jiang, Chunpeng Xia, Kai Xu, Daoying Yuan, Haiying Chen, Bin Zhang, Shuwei LiuAbstractCDKL1 is a cyclin-dependent kinase-like kinase that is highly expressed in diverse types of cancer cells. However, the role of CDKL1 in the chemoresistance of oral squamous cell carcinoma (OSCC) remains largely undefined. Here, we explored the role of CDKL1 in the chemoresistance of the human OSCC cell line CAL27 to hydroxycamptothecin (HCPT). Real-time quantitative polymerase chain reaction and western blotting r...
Source: Molecular and Cellular Probes - February 23, 2019 Category: Molecular Biology Source Type: research
EvaGreen-based real-time PCR assay for sensitive detection of enzootic nasal tumor virus 2
In conclusion, we report that a highly sensitive, specific, and cost-effective EvaGreen-based real-time PCR assay is successful for the rapid detection of ENTV-2. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - February 14, 2019 Category: Molecular Biology Source Type: research
Development of a SYBR green I-based duplex real-time fluorescence quantitative PCR assay for the simultaneous detection of porcine epidemic diarrhea virus and porcine circovirus 3
Publication date: Available online 5 February 2019Source: Molecular and Cellular ProbesAuthor(s): Hao-ying Han, Hui-hua Zheng, Yu Zhao, Run-bo Tian, Peng-Li Xu, Hua-lin Hou, Hong-Ying Chen, Ming-Fan YangAbstractThe development of a rapid, specific, and sensitive SYBR Green I-based duplex real-time quantitative PCR assay is described for the simultaneous detection of porcine epidemic diarrhea virus (PEDV) and porcine circovirus type 3 (PCV3). The assay specifically detected PEDV and PCV3, with no fluorescence detected for other non-targeted pig pathogens. The assay showed a good linear relationship, and the limits of detect...
Source: Molecular and Cellular Probes - February 6, 2019 Category: Molecular Biology Source Type: research
MiR-486-5p inhibits the proliferation of leukemia cells and induces apoptosis through targeting FOXO1
ConclusionmiR-486-5p may inhibit the proliferation of leukemia cells and induce apoptosis through targeting FOXO1. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - February 5, 2019 Category: Molecular Biology Source Type: research
MicroRNA-224 impairs adipogenic differentiation of bovine preadipocytes by targeting LPL
In this study, the potential functions of miR-224 in regulating adipogenic differentiation were explored in bovine preadipocytes. Comparative transcriptome analysis between castrated male cattle with increased intramuscular fat (IMF) and intact male cattle revealed that miR-224 and LPL were abnormally expressed, correlating negatively, and LPL was a predicted target of miR-224. A dual luciferase reporter assay confirmed the negative targeting regulatory relationship between miR-224 and LPL. When miR-224 was either overexpressed or silenced, qRT-PCR showed a negative regulatory effect on LPL. mRNA expression levels of the f...
Source: Molecular and Cellular Probes - January 28, 2019 Category: Molecular Biology Source Type: research
