Point-of-care detection of 16S rRNA of Staphylococcus aureus based on multiple biotin-labeled DNA probes
Publication date: Available online 30 July 2019Source: Molecular and Cellular ProbesAuthor(s): Xiaoliang Zheng, Yue Wang, Shengjun Bu, Zhibao Chen, Jiayu WanAbstractA visual method that combines multiple biotin-labeled DNA probes and lateral-flow nucleic acid biosensor was developed to detect Staphylococcus aureus. The 16S rRNA from Staphyloccocus aureus (S. aureus), coupled with multiple biotin-labeled DNA probes, was functionalized in a signal structure for lateral-flow point-of-care detection. The secondary structure of the 16S rRNA was unwound by two specific capture probes modified by Fam and multiple bridge probes, w...
Source: Molecular and Cellular Probes - July 30, 2019 Category: Molecular Biology Source Type: research
A seminested RT-PCR for molecular genotyping of the Brazilian BR-I Infectious Bronchitis Virus Strain (GI-11)
In this study, 62.24% of 572 organ pools from the 5 regions of Brazil tested positive in a 3′UTR screening, and 84.83% were typed as BR-I IBV. BR-I was detected in the respiratory, digestive and urogenital tracts in pooled samples from all Brazilian geographical regions and in all the breeding systems analyzed. Specificity and sensitivity tests as well as phylogenetic analysis successfully confirmed the expected clustering of the sequences detected by this assay with the BR-I (GI-11) group. The nested PCR described in this study represents a suitable and valuable tool in the diagnosis, epidemiology, monitoring and vaccin...
Source: Molecular and Cellular Probes - July 29, 2019 Category: Molecular Biology Source Type: research
Editorial Board
Publication date: August 2019Source: Molecular and Cellular Probes, Volume 46Author(s): (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - July 28, 2019 Category: Molecular Biology Source Type: research
Publisher note
Publication date: Available online 24 July 2019Source: Molecular and Cellular ProbesAuthor(s): (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - July 25, 2019 Category: Molecular Biology Source Type: research
A novel approach to improving hybrid capture sequencing targeting efficiency
Publication date: Available online 20 July 2019Source: Molecular and Cellular ProbesAuthor(s): WenXiang Lu, Miao Zhu, Yi Chen, Yunfei BaiAbstractAt present, approaches to hybrid capture sequencing have many limitations, from their significant complexity and labor requirements, to their low enrichment efficiency, resulting in their limited utilization. In an effort to overcome these drawbacks, we have developed a novel method that relied upon direct genomic DNA hybridization and single-stranded DNA library preparation. Using this novel protocol, we were able to achieve a targeting efficiency as high as 75%, and we found thi...
Source: Molecular and Cellular Probes - July 21, 2019 Category: Molecular Biology Source Type: research
Detection of coralyne and heparin by polymerase extension reaction using SYBR Green I
Publication date: Available online 16 July 2019Source: Molecular and Cellular ProbesAuthor(s): Changbei Ma, Miangjian Chen, Hailun He, Leilei ChenAbstractPolydeoxyadenosine (poly (dA)) has been extensively applied for detecting many drug molecules. Herein, we developed a sensitive method for detecting coralyne and heparin using a modified DNA probe with poly (dA) at one end. In the absence of coralyne, the DNA probe was digested by the Exonuclease I (Exo I), and therefore the SYBR Green I (SG I) emitted an extremely low fluorescent signal. While coralyne specifically binding to poly (dA) with strong propensity could remark...
Source: Molecular and Cellular Probes - July 18, 2019 Category: Molecular Biology Source Type: research
Evaluation of two aneuploidy screening tests for chorionic villus samples: Multiplex ligation-dependent probe amplification and fluorescence in situ hybridization
Publication date: Available online 15 July 2019Source: Molecular and Cellular ProbesAuthor(s): Tonghua Wu, Yuanchang Zhu, Ling Hong, Qi Lin, Chunmei Chen, Jing Yang, Lijun Ye, Wensi Huang, Yong ZengAbstractThe vast majority of first-trimester pregnancy losses are the consequence of numerical aberrations in fetal chromosomes, which may involve nearly all chromosomes. Although commercial probes for all chromosomes are available for multiplex ligation-dependent probe amplification (MLPA) and fluorescence in situ hybridization (FISH) analyses, their use has rarely been reported for screening all 24 chromosomes for early fetal ...
Source: Molecular and Cellular Probes - July 16, 2019 Category: Molecular Biology Source Type: research
Quantitative PCR evaluation of deletions/duplications identified by array CGH
Publication date: Available online 11 July 2019Source: Molecular and Cellular ProbesAuthor(s): Federica Baldan, Nadia Passon, Silvia Burra, Eliana Demori, Patrizia Dello Russo, Giuseppe DamanteAbstractGenomic deletions/duplications detected by array comparative genomic hybridization (aCGH) should be confirmed by an independent technology. This approach allows also to test, at low cost, inheritance of the imbalance. In the present study we explored the use of quantitative PCR (qPCR) to confirm aCGH-detected potentially clinically relevant imbalances. Only samples with DLRS <0.2 were tested for confirmation. aCGH results...
Source: Molecular and Cellular Probes - July 12, 2019 Category: Molecular Biology Source Type: research
Application of loop-mediated isothermal amplification (LAMP) for rapid detection of Atlantic cod (Gadus morhua), Pacific cod (Gadus macrocephalus) and haddock (Melanogrammus aeglefinus)
Publication date: Available online 8 July 2019Source: Molecular and Cellular ProbesAuthor(s): Yi Wang, Junli Feng, Xiaolan TianAbstractCodfish is a commercially important species of sea fish and plays an important role in the world fishery. In our study, two loop-mediated isothermal amplification (LAMP) assays (real-time fluorescence LAMP and visual LAMP) were established for the identification of three cod species in Gadidae (Gadus morhua, Gadus macrocephalus and Melanogrammus aeglefinus). 12S rDNA gene was used to design primers to distinguish the Gadidae and non-Gadidae species, and the mitochondrial Cytb gene was selec...
Source: Molecular and Cellular Probes - July 9, 2019 Category: Molecular Biology Source Type: research
SATQPCR: Website for statistical analysis of real-time quantitative PCR data
Publication date: Available online 5 July 2019Source: Molecular and Cellular ProbesAuthor(s): Corinne Rancurel, Trang van Tran, Céline Elie, Frédérique HilliouAbstractSATQPCR is a web tool providing statistical analysis of real-time quantitative PCR data including all MIQE rules (gene efficiency, selection of reference genes and normalization with them).Our application is a quick tool that provides to the biologist, graphs as well as statistical tables summarizing their results with the chosen methods (t-test or ANOVA with Tukey test).The application is available at http://satqpcr.sophia.inra.fr with a demo dataset. Sou...
Source: Molecular and Cellular Probes - July 7, 2019 Category: Molecular Biology Source Type: research
Effects of lycopene on ovarian cancer cell line SKOV3 in vitro: Suppressed proliferation and enhanced apoptosis
ConclusionLycopene could inhibit the proliferation of SKOV3 cells and enhance their apoptosis in vitro. The induction of cell apoptosis might be mediated by up-regulating Bax expression and down-regulating Bcl-2 expression. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - July 4, 2019 Category: Molecular Biology Source Type: research
Sp1 promotes cell migration and invasion in oral squamous cell carcinoma by upregulating Annexin A2 transcription
Publication date: Available online 26 June 2019Source: Molecular and Cellular ProbesAuthor(s): Xian-bin Liu, Jing Wang, Ke Li, Xian-nan FanAbstractOral squamous cell carcinoma (OSCC) is a common malignant tumor with high metastatic potential in head and neck. Revealing the mechanism of OSCC metastasis will benefit the prognosis and prevention of OSCC. Sp1 is a transcription factor involved in the progression of several tumors. Annexin A2 functions as an oncogene, and there are three putative Sp1 binding sites in the Annexin A2 promoter region. Therefore, we hypothesized that Sp1 could regulate OSCC metastasis by regulating...
Source: Molecular and Cellular Probes - June 27, 2019 Category: Molecular Biology Source Type: research
The lipid profile of three Malassezia species assessed by Raman spectroscopy and discriminant analysis
Publication date: Available online 25 June 2019Source: Molecular and Cellular ProbesAuthor(s): Chrysoula Petrokilidou, Eleftherios Pavlou, Georgios Gaitanis, Ioannis D. Bassukas, Manolis N. Saridomichelakis, Aristea Velegraki, Nikolaos KourkoumelisAbstractMalassezia yeasts constitute the major eukaryotic cutaneous flora of homoeothermic vertebrates. These lipophilic yeasts are able to cause, trigger, or aggravate common skin diseases under favorable conditions. Species identification and subspecies differentiation is currently based on morphological characteristics, lipid assimilation profile, and molecular tests. Mass spe...
Source: Molecular and Cellular Probes - June 25, 2019 Category: Molecular Biology Source Type: research
Computational design of a chimeric epitope-based vaccine to protect against Staphylococcus aureus infections
Publication date: Available online 22 June 2019Source: Molecular and Cellular ProbesAuthor(s): Nasim Hajighahramani, Mahboobeh Eslami, Manica Negahdaripour, Mohammad Bagher Ghoshoon, Ali Dehshahri, Nasrollah Erfani, Reza Heidari, Ahmad Gholami, Navid Nezafat, Younes GhasemiGraphical abstract (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - June 22, 2019 Category: Molecular Biology Source Type: research
Ultrasound-mediated destruction of vascular endothelial growth factor (VEGF) targeted and paclitaxel loaded microbubbles for inhibition of human breast cancer cell MCF-7 proliferation
ConclusionsThe potential effects of VTPLLM + US on apoptosis of MCF-7 cells suggest that applying VTPLLM + US might be an effective strategy in breast cancer therapies. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - June 20, 2019 Category: Molecular Biology Source Type: research
