GSTO1 regards as a meritorious regulator in cutaneous malignant melanoma cells
ConclusionsTo sum up, our outcomes exhibited that weakening GSTO1 reduced the proliferation and mobility of CMM cells, increased the apoptosis ability of CMM cells, and arrested cell cycle at G1 phase, which can be achieved by affecting the expression of PCNA, p53 and the EMT process. This discovery provided a new perspective for elucidating the mechanism of CMM, and offered theoretical support for searching clinical therapeutic targets in the future. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - September 13, 2019 Category: Molecular Biology Source Type: research
Designing self-assembled peptide nanovaccine against Streptococcus pneumoniae: An in silico strategy
Publication date: Available online 11 September 2019Source: Molecular and Cellular ProbesAuthor(s): Hesam Dorosti, Mahboobeh Eslami, Navid Nezafat, Fardin Fadaei, Younes GhasemiAbstractStreptococcus pneumoniae is the main cause of diseases such as meningitis, pneumoniae and sepsis, especially in children and old people. Due to costly antibiotic treatment, and increasing resistance of pneumococcus, developing high-efficient protective vaccine against this pathogen is an urgent need. Although the pneumoniae polysaccharide vaccine (PPV) and pneumonia conjugate vaccines (PCV) are the efficient pneumococcal vaccine in children ...
Source: Molecular and Cellular Probes - September 12, 2019 Category: Molecular Biology Source Type: research
Development and application of a fiber2 protein-based indirect ELISA for detection of duck adenovirus 3
Publication date: Available online 10 September 2019Source: Molecular and Cellular ProbesAuthor(s): Cuiteng Chen, Chunhe Wan, Shaohua Shi, Longfei Cheng, Zhen Chen, Guanghua Fu, Rongchang Liu, Chunhua Zhu, Yu HuangAbstractDuck adenovirus 3 (DAdV-3) is a newly identified duck adenovirus that has recently emerged in China. The incidence of duck infection caused by this virus is very high, with very large economic losses to the poultry industry. Thus, there is an urgent need for a serological assay for the specific detection of DAdV-3. To this end, prokaryotic expression of the fiber2 protein of DAdV-3 was used as a coating a...
Source: Molecular and Cellular Probes - September 11, 2019 Category: Molecular Biology Source Type: research
miR-1307–3p overexpression inhibits cell proliferation and promotes cell apoptosis by targeting ISM1 in colon cancer
ConclusionsmiR-1307–3p inhibited activation of Wnt3a/β-catenin signaling through targeting downregulation of ISM1, thereby inhibited proliferation and promote apoptosis of COAD cells. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - September 10, 2019 Category: Molecular Biology Source Type: research
Hsa-miR-346 plays a role in the development of sepsis by downregulating SMAD3 expression and is negatively regulated by lncRNA MALAT1
Publication date: Available online 5 September 2019Source: Molecular and Cellular ProbesAuthor(s): Qiuhong Yang, Kaiqi Cao, Guangjun Jin, Jiancheng ZhangAbstractSepsis is a common complication in infection, trauma, and surgery. Severe sepsis has been identified as the leading cause of death in patients suffering from noncardiovascular ailments in intensive care units. In the current study, we used lipopolysaccharide (LPS) to stimulate the mouse macrophage cell line RAW264.7, and investigated the effects of the lncRNA MALAT1/hsa-miR-346/SMAD3 regulatory network on the progression of sepsis. We showed that MALAT1 inhibited R...
Source: Molecular and Cellular Probes - September 7, 2019 Category: Molecular Biology Source Type: research
GR and Foxa1 promote the transcription of ANGPTL4 in bovine adipocytes
In conclusion, GR and Foxa1 were determined to be responsible for the regulation of ANGPTL4 transcription. Our results may provide a basis for further investigation of ANGPTL4 regulation and a reference for improvement of beef quality in cattle. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - September 4, 2019 Category: Molecular Biology Source Type: research
Differential expression of miRNAs regulating NF-κB and STAT3 crosstalk during colitis-associated tumorigenesis
Publication date: Available online 31 August 2019Source: Molecular and Cellular ProbesAuthor(s): Sherien M. El-Daly, Enayat A. Omara, Jihan Hussein, Eman Refaat, Zakaria El-KhayatAbstractInflammatory bowel disease (IBD) is mostly responsible for the development of colitis-associated colon cancer. Of the several signaling pathways involved in colonic inflammation, the activation and crosstalk between NF-κB and STAT3 serve as the pivotal regulatory hubs that regulate epithelial tumorigenesis by linking inflammation with cancer development. Understanding the molecular mechanisms regulating the crosstalk between NF-κB and ST...
Source: Molecular and Cellular Probes - September 1, 2019 Category: Molecular Biology Source Type: research
The ribosomal intergenic spacer (IGS) in the potato and tobacco cyst nematodes, Globodera pallida, G. rostochiensis and G. tabacum
In this study, the first flanking portion of the IGS was amplified, cloned and sequenced from PCN and TCN. Primers were then designed to amplify the whole IGS sequence. PCR amplification of IGS from G. tabacum, G. pallida, and G. rostochiensis yielded respectively: a single amplicon of 3 kb, three amplicons sized 2.5, 2.6 and 2.9 kb, and two amplicons sized 2.8 and 2.9 kb. Results showed that Globodera spp. has more than one variant copy of the IGS, with both long and short repetitive DNA elements. An approximately 400 bp long region without any internal repetitive elements, were identified in a position between the ...
Source: Molecular and Cellular Probes - August 28, 2019 Category: Molecular Biology Source Type: research
PRPF4 is a novel therapeutic target for the treatment of breast cancer by influencing growth, migration, invasion, and apoptosis of breast cancer cells via p38 MAPK signaling pathway
In conclusion, the PRPF4 gene plays an important role in the growth of breast cancer cells and is therefore a potential therapeutic target. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - August 23, 2019 Category: Molecular Biology Source Type: research
A duplex PCR assay for the simultaneous detection and differentiation of Muscovy duck parvovirus and goose parvovirus
In conclusion, a duplex PCR assay for the simultaneous detection and differentiation of MDPV and GPV was established using only three highly specific primers. Our finding suggested that country-wide vaccination with MDPV and GPV vaccines in waterfowls are necessary. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - August 21, 2019 Category: Molecular Biology Source Type: research
miR-34a and miR-29b as indicators for prognosis of treatment-free survival of chronic lymphocytic leukemia patients in Chinese Uygur and Han populations
Publication date: Available online 16 August 2019Source: Molecular and Cellular ProbesAuthor(s): Yan Li, Min Mao, Hong Liu, Xiaomin Wang, Zhen Kou, Yuling Nie, Yichun Wang, Zengsheng Wang, Qin Huang, Tao Lang, Zailinuer Gu, Li An, Xiaoyan Zhang, Lin FuAbstractThe abnormal expression of miRNAs may play critical roles in the occurrence, development and prognosis of chronic lymphocytic leukemia (CLL), with potential ethnic differences being involved. p53 and immunoglobulin heavy chain variable region gene (IGVH) mutations were monitored and miRNA profile screening of CD19 + cells from Uygur CLL patients was performed, ana...
Source: Molecular and Cellular Probes - August 17, 2019 Category: Molecular Biology Source Type: research
Rapid and quantitative detection of viable emetic Bacillus cereus by PMA-qPCR assay in milk
Publication date: Available online 16 August 2019Source: Molecular and Cellular ProbesAuthor(s): Ping Zhou, Guoyang Xie, Taobo Liang, Bei Yu, Zoraida Aguilar, Hengyi XuAbstractEmetic Bacillus cereus is one of the causative agents of foodborne diseases which can cause vomiting-type food poisoning after ingestion of contaminated food. To minimize B. cereus food poisoning, propidium monoazide (PMA) combined with quantitative polymerase chain reaction (qPCR) called PMA-qPCR was applied for detecting viable emetic B. cereus in milk. The cereulide synthetase gene of emetic B. cereus (cesB) was chosen for the primer, and PMA trea...
Source: Molecular and Cellular Probes - August 17, 2019 Category: Molecular Biology Source Type: research
Development of SYBR Green real-time PCR and nested RT-PCR for the detection of Potato Mop-top Virus (PMTV) and viral surveys in Progeny tubers derived from PMTV infected Potato tubers
In this study, a new SYBR Green qPCR (qRT-PCR) and a nested RT-PCR (nRT-PCR) were developed to detect Potato mop-top virus (PMTV) in potato tuber tissues. The SYBR Green qRT-PCR and nRT-PCR assays were approximately 104- and 103- fold more sensitive than the conventional RT-PCR assay. The progeny tubers derived from PMTV-infected potato tubers were tested by conventional RT-PCR, SYBR Green qRT-PCR and nRT-PCR assays. Of the 17 samples, 9 (52.9%) were positive for PMTV by conventional RT-PCR, 11 (64.7%) were positive by nRT-PCR, and 17 (100%) were positive by SYBR Green qRT-PCR. Compared to nRT-PCR, SYBR Green qRT-PCR was s...
Source: Molecular and Cellular Probes - August 16, 2019 Category: Molecular Biology Source Type: research
Dual priming oligonucleotide (DPO)-based real-time RT-PCR assay for accurate differentiation of four major viruses causing porcine viral diarrhea
Publication date: Available online 12 August 2019Source: Molecular and Cellular ProbesAuthor(s): Shuo Jia, Baohua Feng, Zhuo Wang, Yingying Ma, Xuwen Gao, Yanping Jiang, Wen Cui, Xinyuan Qiao, Lijie Tang, Yijing Li, Li Wang, Yigang XuAbstractCurrently in China, porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), porcine rotavirus (PoRV), and porcine deltacoronavirus (PDCoV) are the major causes of porcine viral diarrhea, and mixed infections in clinics are common, resulting in significant economic losses in pig industry. Here, a dual priming oligonucleotide (DPO)-based multiplex real-time SY...
Source: Molecular and Cellular Probes - August 13, 2019 Category: Molecular Biology Source Type: research
Establishment of a recombinase polymerase amplification (RPA) assay for the detection of Brucella spp. Infection
In conclusion, RPA is a useful and convenient field and point of care test for brucellosis. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - August 10, 2019 Category: Molecular Biology Source Type: research
