Development of real-time PCR assays for single and simultaneous detection of infectious bursal disease virus and chicken anemia virus
Publication date: Available online 14 November 2018Source: Molecular and Cellular ProbesAuthor(s): Claudia Techera, Gonzalo Tomás, Yanina Panzera, Alejandro Banda, Paula Perbolianachis, Ruben Pérez, Ana MarandinoAbstractInfectious bursal disease virus (IBDV) and chicken anemia virus (CAV) cause relevant immunosuppressive diseases in poultry. Clinical diagnosis of these viruses is challenging given the different disease presentations and the frequent occurrence of co-infections with other pathogens. Here, we standardized and validated simplex and duplex RT-qPCR assays for the straightforward detection of IBDV and CAV. The...
Source: Molecular and Cellular Probes - November 15, 2018 Category: Molecular Biology Source Type: research
Genes and transcription factors related to the adverse effects of maternal type I diabetes mellitus on fetal development
ConclusionsSome genes (e.g. CXCL12 and CCL5) and their TFs were significantly and abnormally regulated in the umbilical cord tissue from the pregnancies of T1DM mothers compared to that from non-T1DM mothers. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - November 15, 2018 Category: Molecular Biology Source Type: research
Rapid and sensitive detection of Amphidinium carterae by loop-mediated isothermal amplification combined with a chromatographic lateral-flow dipstick
In conclusion, the developed LAMP-LFD assay can be used as a reliable and simple method of detecting A. carterae. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - November 11, 2018 Category: Molecular Biology Source Type: research
Editorial Board
Publication date: December 2018Source: Molecular and Cellular Probes, Volume 42Author(s): (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - November 11, 2018 Category: Molecular Biology Source Type: research
Establishment of rapid detection method and surveillance of budgerigar fledgling disease virus using a TaqMan Real-Time PCR
In this study, we developed a TaqMan Real-time PCR assay to detect BFDV by targeting a conserved region in VP1 gene. The detection limit of the assay was 30 DNA gene copies, 1000 times more sensitive than conventional PCR. The coefficients of variation were less than 1.09% in either intra- or inter-assays, indicating high reproducibility. By using this method, the prevalence of BFDV in China was evaluated. 56 feces samples were collected from four psittacine birds breeding facilities in China. The results showed 28 out of 56 samples were positive for BFDV in Real-Time PCR assay, while only 19 samples were positive in PCR a...
Source: Molecular and Cellular Probes - November 9, 2018 Category: Molecular Biology Source Type: research
