A novel approach to improving hybrid capture sequencing targeting efficiency

Publication date: Available online 20 July 2019Source: Molecular and Cellular ProbesAuthor(s): WenXiang Lu, Miao Zhu, Yi Chen, Yunfei BaiAbstractAt present, approaches to hybrid capture sequencing have many limitations, from their significant complexity and labor requirements, to their low enrichment efficiency, resulting in their limited utilization. In an effort to overcome these drawbacks, we have developed a novel method that relied upon direct genomic DNA hybridization and single-stranded DNA library preparation. Using this novel protocol, we were able to achieve a targeting efficiency as high as 75%, and we found this approach to overall be an efficient and simple approach to DNA library construction.
Source: Molecular and Cellular Probes - Category: Molecular Biology Source Type: research