Mitochondrial permeability transition pore in sea urchin female gametes
Publication date: Available online 25 July 2018Source: Mechanisms of DevelopmentAuthor(s): Elis Torrezan-Nitao, Regina Celia Bressan Queiroz Figueiredo, Luis Fernando Marques-SantosAbstractMitochondrial permeability transition pore (MPTP) has been associated to calcium homeostasis and reactive oxygen species (ROS) generation in several cell types. While extensively investigated in somatic cells, there are few data regarding MPTP phenomenon in gametes. The aim of the present work was to investigate MPTP occurrence in sea urchin female gametes. The protonophores CCCP and FCCP, and the Ca2+ ionophore ionomycin, were used as p...
Source: Mechanisms of Development - July 25, 2018 Category: Biology Source Type: research
Editorial Board
Publication date: August 2018Source: Mechanisms of Development, Volume 152Author(s): (Source: Mechanisms of Development)
Source: Mechanisms of Development - July 25, 2018 Category: Biology Source Type: research
Pdgf1aa regulates zebrafish neural crest cells migration through Hif-1 in an oxygen-independent manner
Publication date: Available online 18 July 2018Source: Mechanisms of DevelopmentAuthor(s): J. Espina, C. Marchant, G.V. De Ferrari, A.E. ReyesAbstractThe transcription factor Hif-1α regulates epithelial to mesenchymal transition and neural crest cell chemotaxis in Xenopus. Hif-1α is only stabilised under low oxygen levels, and the in vivo stabilisation of this factor in neural crest cells is poorly understood. Multiple oxygen-independent Hif-1α regulators have been described in cell cultures and cancer models. Among these, the PDGF pathway has been linked to neural crest development. The present study established a conn...
Source: Mechanisms of Development - July 19, 2018 Category: Biology Source Type: research
Transcriptional cross-regulation of Irre Cell Recognition Module (IRM) members in the Drosophila pupal retina
Publication date: Available online 18 July 2018Source: Mechanisms of DevelopmentAuthor(s): Maiaro Cabral Rosa Machado, Felipe Berti Valer, Carlos Antonio Couto Lima, Ricardo Guelerman Pinheiro RamosAbstractCell adhesion molecules play a central role in morphogenesis, as they mediate the complex range of interactions between different cell types that result in their arrangement in multicellular organs and tissues. How their coordinated dynamic expression in space and time - an essential requirement for their function - is regulated at the genomic and transcriptional levels constitutes an important, albeit still little under...
Source: Mechanisms of Development - July 18, 2018 Category: Biology Source Type: research
A catenin-dependent balance between N-cadherin and E-cadherin controls neuroectodermal cell fate choices
Publication date: August 2018Source: Mechanisms of Development, Volume 152Author(s): Crystal D. Rogers, Lisa K. Sorrells, Marianne E. BronnerAbstractCharacterizing endogenous protein expression, interaction and function, this study identifies in vivo interactions and competitive balance between N-cadherin and E-cadherin in developing avian (Gallus gallus) neural and neural crest cells. Numerous cadherin proteins, including neural cadherin (Ncad) and epithelial cadherin (Ecad), are expressed in the developing neural plate as well as in neural crest cells as they delaminate from the newly closed neural tube. To clarify indep...
Source: Mechanisms of Development - July 15, 2018 Category: Biology Source Type: research
The extraordinary biology and development of marsupial frogs (Hemiphractidae) in comparison with fish, mammals, birds, amphibians and other animals
Publication date: Available online 3 January 2018Source: Mechanisms of DevelopmentAuthor(s): Eugenia M. del PinoAbstractThe study of oogenesis and early development of frogs belonging to the family Hemiphractidae provide important comparison to the aquatic development of other frogs, such as Xenopus laevis, because reproduction on land characterizes the Hemiphractidae. In this review, the multinucleated oogenesis of the marsupial frog Flectonotus pygmaeus (Hemiphractidae) is analyzed and interpreted. In addition, the adaptations associated with the incubation of embryos in the pouch of the female marsupial frog Gastrotheca...
Source: Mechanisms of Development - July 11, 2018 Category: Biology Source Type: research
Metabo-Devo: A metabolic perspective of development
Publication date: Available online 21 February 2018Source: Mechanisms of DevelopmentAuthor(s): Lautaro Gándara, Pablo WappnerAbstractIn the last years, several reports have established the notion that metabolism is not just a housekeeping process, but instead an active effector of physiological changes. The idea that the metabolic status may rule a wide range of phenomena in cell biology is starting to be broadly accepted. Thus, current developmental biology has begun to describe different ways by which the metabolic profile of the cell and developmental programs of the organism can crosstalk. In this review, we discuss m...
Source: Mechanisms of Development - July 11, 2018 Category: Biology Source Type: research
Emerging role of dynamic RNA modifications during animal development
Publication date: Available online 11 April 2018Source: Mechanisms of DevelopmentAuthor(s): Estefanía Sánchez-Vásquez, Nagif Alata Jimenez, Nicolás A. Vázquez, Pablo H. Strobl-MazzullaAbstractThe central dogma of molecular biology statically says that the information flows from DNA to messenger RNA to protein. But the recent advances in mass spectrometry and high throughput technology have helped the scientists to view RNA as little more than a courier of genetic information encoded in the DNA. The dynamics of RNA modifications in coding and non-coding RNAs are just emerging as a carrier of non-genetic information, un...
Source: Mechanisms of Development - July 11, 2018 Category: Biology Source Type: research
Regulation mechanisms and implications of sperm membrane hyperpolarization
Publication date: Available online 22 April 2018Source: Mechanisms of DevelopmentAuthor(s): Carla Ritagliati, Carolina Baro Graf, Cintia Stival, Dario KrapfAbstractMammalian sperm are unable to fertilize the egg immediately after ejaculation. In order to gain fertilization competence, they need to undergo a series of biochemical and physiological modifications inside the female reproductive tract, known as capacitation. Capacitation correlates with two essential events for fertilization: hyperactivation, an asymmetric and vigorous flagellar motility, and the ability to undergo the acrosome reaction. At a molecular level, c...
Source: Mechanisms of Development - July 11, 2018 Category: Biology Source Type: research
The biology and evolution of the Dilp8-Lgr3 pathway: A relaxin-like pathway coupling tissue growth and developmental timing control
Publication date: Available online 30 April 2018Source: Mechanisms of DevelopmentAuthor(s): Alisson M. Gontijo, Andres GarelliAbstractMany insects, like cockroaches, moths, and flies, can regenerate tissues by extending the growth-competent phases of their life cycle. The molecular and cellular players mediating this coordination between tissue growth and developmental timing have been recently discovered in Drosophila. The insulin/relaxin-like peptide, Dilp8, was identified as a factor communicating abnormal growth status of Drosophila larval imaginal discs to the neuroendocrine centers that control the timing of the onse...
Source: Mechanisms of Development - July 11, 2018 Category: Biology Source Type: research
CTCF knockout reveals an essential role for this protein during the zebrafish development
In conclusion, CTCF is a ubiquitous factor during the zebrafish development, which regulates the correct formation of different structures of the embryo, and its deregulation impacts on essential cell survival genes. Overall, this work provides a basis to look for the particular functions of CTCF in the different developing tissues and organs of the zebrafish. (Source: Mechanisms of Development)
Source: Mechanisms of Development - July 11, 2018 Category: Biology Source Type: research
Imaging transcription factors dynamics with advanced fluorescence microscopy methods
Publication date: Available online 10 May 2018Source: Mechanisms of DevelopmentAuthor(s): Paula Verneri, Juan José Romero, María Cecilia De Rossi, Yanina Alvarez, Camila Oses, Alejandra Guberman, Valeria LeviAbstractPluripotent stem cells (PSCs) are capable of self-renewing and producing all cell types derived from the three germ layers in response to developmental cues, constituting an important promise for regenerative medicine. Pluripotency depends on specific transcription factors (TFs) that induce genes required to preserve the undifferentiated state and repress other genes related to differentiation. The transcript...
Source: Mechanisms of Development - July 11, 2018 Category: Biology Source Type: research
G-quadruplex in animal development: Contribution to gene expression and genomic heterogeneity
Publication date: Available online 18 May 2018Source: Mechanisms of DevelopmentAuthor(s): Pablo Armas, Nora Beatriz CalcaterraAbstractDuring animal development, gene expression is orchestrated by specific and highly evolutionarily conserved mechanisms that take place accurately, both at spatial and temporal levels. The last decades have provided compelling evidence showing that chromatin state plays essential roles in orchestrating most of the stages of development. The DNA molecule can adopt alternative structures different from the helical duplex architecture. G-rich DNA sequences can fold as intrastrand quadruple helix ...
Source: Mechanisms of Development - July 11, 2018 Category: Biology Source Type: research
Regulation and function of p53: A perspective from Drosophila studies
Publication date: Available online 23 May 2018Source: Mechanisms of DevelopmentAuthor(s): María Clara Ingaramo, Juan A. Sánchez, Andrés DekantyAbstractTp53 is a central regulator of cellular responses to stress and one of the most frequently mutated genes in human cancers. P53 is activated by a myriad of stress signals and drives specific cellular responses depending on stress nature, cell type and cellular context. Additionally to its classical functions in regulating cell cycle arrest, apoptosis and senescence, newly described non-canonical functions of p53 are increasingly coming under the spotlight as important func...
Source: Mechanisms of Development - July 11, 2018 Category: Biology Source Type: research
The neuromuscular junction of Xenopus tadpoles: Revisiting a classical model of early synaptogenesis and regeneration
Publication date: Available online 26 May 2018Source: Mechanisms of DevelopmentAuthor(s): Francisca Bermedo-García, Jorge Ojeda, Emilio E. Méndez-Olivos, Sylvain Marcellini, Juan Larraín, Juan Pablo HenríquezAbstractThe frog neuromuscular junction (NMJ) has been extensively used as a model system to dissect the mechanisms involved in synapse formation, maturation, maintenance, regeneration, and function. Early NMJ synaptogenesis relies on a combination of cell-autonomous and interdependent pre/postsynaptic communication processes. Due to their transparency, comparatively easy manipulation, and remarkable regenerative a...
Source: Mechanisms of Development - July 11, 2018 Category: Biology Source Type: research
