Zebrafish Pax1a and Pax1b are required for pharyngeal pouch morphogenesis and ceratobranchial cartilage development
Publication date: Available online 13 February 2020Source: Mechanisms of DevelopmentAuthor(s): Yu-Hsiu Liu, Tz-Chi Lin, Sheng-Ping L. HwangAbstractPharyngeal arches are derived from all three germ layers and molecular interactions among the tissue types are required for proper development of subsequent pharyngeal cartilages; however, the mechanisms underlying this process are not fully described. Here we report that in zebrafish, Pax1a and Pax1b have overlapping and essential functions in pharyngeal pouch morphogenesis and subsequent ceratobranchial cartilage development. Both pax1a and pax1b are co-expressed in pharyngeal...
Source: Mechanisms of Development - February 13, 2020 Category: Biology Source Type: research
Transcriptome analysis of axolotl oropharyngeal explants during taste bud differentiation stages
Publication date: Available online 7 February 2020Source: Mechanisms of DevelopmentAuthor(s): Priya Kohli, Lauren Marazzi, Deborah EastmanAbstractThe Mexican salamander, Ambystoma mexicanum (Axolotl), is an excellent vertebrate model system to understand development and regeneration. Studies in axolotl embryos have provided important insights into taste bud development. Taste bud specification and determination occur in the oropharyngeal endoderm of axolotl embryos during gastrulation and neurulation, respectively, whereas taste bud innervation and taste cell differentiation occur later in development. Axolotl embryos are ...
Source: Mechanisms of Development - February 8, 2020 Category: Biology Source Type: research
Disconnect between the developing eye and craniofacial prominences in the avian embryo
Publication date: Available online 7 February 2020Source: Mechanisms of DevelopmentAuthor(s): Jamil Jomaa, Jessica Martínez-Vargas, Shadya Essaili, Nida Haider, John AbramyanAbstractIn the amniote embryo, the upper jaw and nasal cavities form through coordinated outgrowth and fusion of craniofacial prominences. Adjacent to the embryonic prominences are the developing eyes, which abut the maxillary and lateral nasal prominences. The embryos of extant sauropsids (birds and nonavian reptiles) develop particularly large eyes in comparison to mammals, leading researchers to propose that the developing eye may facilitate outgro...
Source: Mechanisms of Development - February 8, 2020 Category: Biology Source Type: research
Claudin-7b and Claudin-h are required for controlling cilia morphogenesis in the zebrafish kidney
Publication date: Available online 27 December 2019Source: Mechanisms of DevelopmentAuthor(s): Miaomiao Jin, Donglian Wang, Wenyan Xu, Hong Wang, Ying CaoAbstractClaudins are a family of proteins which are the most important components of the tight junctions. The location of Claudins on the renal tubule epithelial determines its paracellular transport characteristics, but whether Claudins have other functions in kidneys remains still unclear. Here, we showed that the transcripts encoding two Claudin family proteins, claudin-7b (cldn-7b) and claudin-h (cldn-h), were expressed in the transporting cells in the zebrafish prone...
Source: Mechanisms of Development - December 28, 2019 Category: Biology Source Type: research
Functional analysis of engrailed in Tribolium segmentation
Publication date: Available online 26 November 2019Source: Mechanisms of DevelopmentAuthor(s): Jinsung Lim, Chong Pyo ChoeAbstractThe segment-polarity gene engrailed is required for segmentation in the early Drosophila embryo. Loss of Engrailed function results in segmentation defects that vary in severity from pair-rule phenotypes to a lawn phenotype lacking in obvious of segmentation. During segmentation, Engrailed is expressed in stripes with a single segmental periodicity in Drosophila, which is conserved in all arthropods examined so far. To define segments, the segmental stripes of Engrailed induce the segmental stri...
Source: Mechanisms of Development - November 26, 2019 Category: Biology Source Type: research
Editorial Board
Publication date: December 2019Source: Mechanisms of Development, Volume 160Author(s): (Source: Mechanisms of Development)
Source: Mechanisms of Development - November 26, 2019 Category: Biology Source Type: research
Regulation of Numb during planar cell polarity establishment in the Drosophila eye
Publication date: Available online 31 October 2019Source: Mechanisms of DevelopmentAuthor(s): Pedro M. Domingos, Andreas Jenny, Keon F. Combie, David del Alamo, Marek Mlodzik, Hermann Steller, Bertrand MollereauAbstractThe establishment of planar cell polarity (PCP) in the Drosophila eye requires correct specification of the R3/R4 pair of photoreceptor cells, determined by a Frizzled mediated signaling event that specifies R3 and induces Delta to activate Notch signaling in the neighboring cell, specifying it as R4. Here, we investigated the role of the Notch signaling negative regulator Numb in the specification of R3/R4 ...
Source: Mechanisms of Development - November 1, 2019 Category: Biology Source Type: research
Editorial “MOD”
Publication date: Available online 21 October 2019Source: Mechanisms of DevelopmentAuthor(s): Roberto Mayor (Source: Mechanisms of Development)
Source: Mechanisms of Development - October 22, 2019 Category: Biology Source Type: research
Initiation and early growth of the skull vault in zebrafish
Publication date: Available online 20 October 2019Source: Mechanisms of DevelopmentAuthor(s): Michelle Kanther, Alexandra Scalici, Azman Rashid, Kelly Miao, Ella Van Deventer, Shannon FisherAbstractThe zebrafish offers powerful advantages as a model system for examining the growth of the skull vault and the formation of cranial sutures. The zebrafish is well suited for large–scale genetic screens, available in large numbers, and continual advances in genetic engineering facilitate precise modeling of human genetic disorders. Most importantly, zebrafish are continuously accessible for imaging during critical periods of sk...
Source: Mechanisms of Development - October 22, 2019 Category: Biology Source Type: research
The discovery of the fundamental role of VEGF in the development of the vascular system
This article summarizes the fundamental contributions that have been made in the early days of angiogenesis research to define the involvement of VEGF/VEGFRs in the developing vasculature.Graphical abstract (Source: Mechanisms of Development)
Source: Mechanisms of Development - October 20, 2019 Category: Biology Source Type: research
In vivo assessment of gonad status, secondary sex characteristics and spawning in transparent Casper zebrafish
In this study, the effects of DES on reproduction were determined in a novel way by using transparent Casper zebrafish that allow direct visualization of gonad status over time. Changes in gonad status with DES treatment were correlated with effects on secondary sex characteristics (i.e., genital vent size and breeding tubercles) spawning and embryo production. The results suggest that the Casper zebrafish is a useful model for studying dynamics of reproductive events in vertebrate gonads in vivo and for determining effects of EDCs on zebrafish reproduction. (Source: Mechanisms of Development)
Source: Mechanisms of Development - October 19, 2019 Category: Biology Source Type: research
The Hedgehog receptor Patched1 regulates proliferation, neurogenesis, and axon guidance in the embryonic spinal cord
Publication date: Available online 18 October 2019Source: Mechanisms of DevelopmentAuthor(s): Angelo Iulianella, Danielle Stanton-TurcotteAbstractThe formation of the vertebrate nervous system depends on the complex interplay of morphogen signaling pathways and cell cycle progression to establish distinct cell fates. The Sonic hedgehog (Shh) signaling pathway is well understood to promote ventral cell fates in the developing spinal cord. A key regulator of Shh signaling is its receptor Patched1 (Ptch1). However, because the Ptch1 null mutation is lethal early in mouse embryogenesis, its role in controlling cell cycle progr...
Source: Mechanisms of Development - October 19, 2019 Category: Biology Source Type: research
Editorial Board
Publication date: October 2019Source: Mechanisms of Development, Volume 159Author(s): (Source: Mechanisms of Development)
Source: Mechanisms of Development - September 21, 2019 Category: Biology Source Type: research
An in vitro model of region-specific rib formation in chick axial skeleton: Intercellular interaction between somite and lateral plate cells
Publication date: Available online 4 September 2019Source: Mechanisms of DevelopmentAuthor(s): Kaoru Matsutani, Koji Ikegami, Hirohiko AoyamaAbstractThe axial skeleton is divided into different regions based on its morphological features. In particular, in birds and mammals, ribs are present only in the thoracic region. The axial skeleton is derived from a series of somites. In the thoracic region of the axial skeleton, descendants of somites coherently penetrate into the somatic mesoderm to form ribs. In regions other than the thoracic, descendants of somites do not penetrate the somatic lateral plate mesoderm. We perform...
Source: Mechanisms of Development - September 5, 2019 Category: Biology Source Type: research
EGL-38/Pax coordinates development in the Caenhorhabditis elegans egg-laying system through EGF pathway dependent and independent functions
Publication date: Available online 6 August 2019Source: Mechanisms of DevelopmentAuthor(s): Allison M. Webb Chasser, Ryan W. Johnson, Helen M. ChamberlinAbstractPaired box (Pax) proteins function as regulators of coordinated development in organogenesis by controlling factors such as cell growth and differentiation necessary to organize multiple cell types into a single, cohesive organ. Previous work has suggested that Pax transcription factors may regulate diverse cell types through participation in inductive cell-to-cell signaling, which has not been well explored. Here we show that EGL-38, a Pax2/5/8 ortholog, coordinat...
Source: Mechanisms of Development - August 7, 2019 Category: Biology Source Type: research
