Journal of Molecular Microbiology and Biotechnology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Evidence of Interruption of the comM Gene in a Large Series of Clinical Isolates of Multidrug-Resistant Acinetobacter baumannii 
This study demonstrated that thecomM gene is targeted by transposons in Iranian MDR-AB isolates belonging to different GCs. The data also showed that the carriage of interruptedcomM is not exclusive to MDR isolates ofA. baumannii.J Mol Microbiol Biotechnol 2016;26:410-413 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - September 15, 2016 Category: Microbiology Source Type: research

Proteomic Analyses Uncover the Mechanisms Underlying Antibiotic Resistance Differences among Three Acinetobacter baumannii Isolates
This study aimed to investigate the molecular mechanisms underlying the antibiotic resistance difference among threeAcinetobacter baumannii isolates. FiftyA. baumannii isolates were first subjected to an antimicrobial susceptibility test, then three isolates differing in antibiotic resistance were selected and subjected to iTRAQ (isobaric tags for relative and absolute quantification)-based proteomics analysis. Differential proteins among the threeA. baumannii isolates were further identified and subjected to gene ontology functional enrichment analysis. A resistant isolate (A1), a less resistant one (A8) and a susceptible...
Source: Journal of Molecular Microbiology and Biotechnology - September 15, 2016 Category: Microbiology Source Type: research

Systems Perspective of Morbillivirus Replication
Systems biology refers to system-wide changes in biological components such as RNA/DNA (genomics), protein (proteomics) and lipids (lipidomics). In this review, we provide comprehensive information about morbillivirus replication. Besides discussing the role of individual viral/host proteins in virus replication, we also discuss how systems-level analyses could improve our understanding of morbillivirus replication, host-pathogen interaction, immune response and disease resistance. Finally, we discuss how viroinformatics is likely to provide important insights for understanding genome-genome, genome-protein and protein-pro...
Source: Journal of Molecular Microbiology and Biotechnology - September 10, 2016 Category: Microbiology Source Type: research

Transport and Catabolism of Pentitols by Listeria monocytogenes
Transposon insertion intoListeria monocytogenes lmo2665, which encodes an EIIC of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS), was found to preventD-arabitol utilization. We confirm this result with a deletion mutant and show that Lmo2665 is also required forD-xylitol utilization. We therefore called this protein EIICAxl. Both pentitols are probably catabolized via the pentose phosphate pathway (PPP) becauselmo2665 belongs to an operon, which encodes the three PTSAxl components, two sugar-P dehydrogenases, and most PPP enzymes. The two dehydrogenases oxidize the pentitol-phosphates produced during ...
Source: Journal of Molecular Microbiology and Biotechnology - August 24, 2016 Category: Microbiology Source Type: research

Analysis of 58 Families of Holins Using a Novel Program, PhyST
We have designed a freely accessible program, PhyST, which allows the automated characterization of any family of homologous proteins within the Transporter Classification Database. The program performs an NCBI-PSI-BLAST search and reports (1) the average protein sequence length with standard deviations, (2) the average predicted number of transmembrane segments, (3) the total number of homologues retrieved, (4) a quantitative list of all source phyla, and (5) potential fusion proteins of sizes considerably exceeding the average size of the proteins retrieved. We have applied this program to 58 families of holins, and the ...
Source: Journal of Molecular Microbiology and Biotechnology - August 23, 2016 Category: Microbiology Source Type: research

Expression and Biochemical Characterization of a Thermostable Branching Enzyme from Geobacillus thermoglucosidans 
In this study, theGeobacillus thermoglucosidans gene-encoding branching enzyme was expressed inEscherichia coliBL21 (DE3) and the protein was isolated and characterized.G. thermoglucosidansbranching enzyme is a thermostable enzyme with an optimal reaction temperature of nearly 60 °C and a half-life at 65°C of approximately 1.1 h. The activity of the recombinant enzyme is optimal at pH 7.5, with broad stability between pH 5.5 and 9.0. Its thermostability, relatively broad pH stability and optimal temperature near the temperature at which starch begins to gelatinize may make it easy to use in industrial production. Further...
Source: Journal of Molecular Microbiology and Biotechnology - August 17, 2016 Category: Microbiology Source Type: research

Peptide Selectivity of the Proton-Coupled Oligopeptide Transporter from Neisseria meningitidis 
This study provides important novel information towards understanding how these transporters recognize their substrates.J Mol Microbiol Biotechnol 2016;26:312-319 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - August 17, 2016 Category: Microbiology Source Type: research

The Number, Organization, and Size of Polymorphic Membrane Protein Coding Sequences as well as the Most Conserved Pmp Protein Differ within and across Chlamydia Species
Variation is a central trait of the polymorphic membrane protein (Pmp) family. The number of pmp coding sequences differs between Chlamydia species, but it is unknown whether the number of pmp coding sequences is constant within a Chlamydia species. The level of conservation of the Pmp proteins has previously only been determined for Chlamydia trachomatis. As different Pmp proteins might be indispensible for the pathogenesis of different Chlamydia species, this study investigated the conservation of Pmp proteins both within and across ...
Source: Journal of Molecular Microbiology and Biotechnology - July 28, 2016 Category: Microbiology Source Type: research

Systematic Exploration of the Glycoproteome of the Beneficial Gut Isolate Lactobacillus rhamnosus GG
Glycoproteins form an interesting class of macromolecules involved in bacterial-host interactions, but they are not yet widely explored in Gram-positive and beneficial species. Here, an integrated and widely applicable approach was followed to identify putative bacterial glycoproteins, combining proteome fractionation with 2D protein and glycostained gels and lectin blots. This approach was validated for the microbiota isolate Lactobacillus rhamnosus GG. The approach resulted in a list of putative glycosylated proteins receiving a ‘glycosylation score'. Ultimately, we could identify 41 unique glycosylated p...
Source: Journal of Molecular Microbiology and Biotechnology - July 28, 2016 Category: Microbiology Source Type: research

The Number, Organization, and Size of Polymorphic Membrane Protein Coding Sequences as well as the Most Conserved Pmp Protein Differ within and across Chlamydia Species
Variation is a central trait of the polymorphic membrane protein (Pmp) family. The number of pmp coding sequences differs between Chlamydia species, but it is unknown whether the number of pmp coding sequences is constant within a Chlamydia species. The level of conservation of the Pmp proteins has previously only been determined for Chlamydia trachomatis. As different Pmp proteins might be indispensible for the pathogenesis of different Chlamydia species, this study investigated the conservation of Pmp proteins both within and across ...
Source: Journal of Molecular Microbiology and Biotechnology - July 27, 2016 Category: Microbiology Source Type: research

Soluble Prokaryotic Expression and Purification of Human Interferon Alpha-2b Using a Maltose-Binding Protein Tag
In this study, seven N-terminal fusion partners - hexahistidine (His6), thioredoxin, glutathione S -transferase (GST), maltose-binding protein (MBP), N-utilization substance protein A, protein disulfide bond isomerase (PDI), and b'a' domain of PDI - were tested for soluble overexpression of codon-optimized IFN α-2b in E. coli . Low temperature increased the expression level of all of the tagged proteins except for the GST fusion. All the tags, except for His6 and GST, improved solubility. We purified IFN α-2b from the MBP-tagged fusion using immobilized metal affinity chromatography and anion ex...
Source: Journal of Molecular Microbiology and Biotechnology - July 27, 2016 Category: Microbiology Source Type: research

Systematic Exploration of the Glycoproteome of the Beneficial Gut Isolate Lactobacillus rhamnosus GG
Glycoproteins form an interesting class of macromolecules involved in bacterial-host interactions, but they are not yet widely explored in Gram-positive and beneficial species. Here, an integrated and widely applicable approach was followed to identify putative bacterial glycoproteins, combining proteome fractionation with 2D protein and glycostained gels and lectin blots. This approach was validated for the microbiota isolate Lactobacillus rhamnosus GG. The approach resulted in a list of putative glycosylated proteins receiving a ‘glycosylation score'. Ultimately, we could identify 41 unique glycosylated p...
Source: Journal of Molecular Microbiology and Biotechnology - July 27, 2016 Category: Microbiology Source Type: research

Transport and Catabolism of Carbohydrates by Neisseria meningitidis
We identified the genes encoding the proteins for the transport of glucose and maltose in Neisseria meningitidis strain 2C4-3. A mutant deleted for NMV_1892 (glcP) no longer grew on glucose and deletion of NMV_0424 (malY) prevented the utilization of maltose. We also purified and characterized glucokinase and α-phosphoglucomutase, which catalyze early catabolic steps of the two carbohydrates. N. meningitidis catabolizes the two carbohydrates either via the Entner-Doudoroff (ED) pathway or the pentose phosphate pathway, thereby forming glycer...
Source: Journal of Molecular Microbiology and Biotechnology - July 26, 2016 Category: Microbiology Source Type: research

Transport and Catabolism of Carbohydrates by Neisseria meningitidis 
We identified the genes encoding the proteins for the transport of glucose and maltose in Neisseria meningitidis strain 2C4-3. A mutant deleted for NMV_1892 (glcP) no longer grew on glucose and deletion of NMV_0424 (malY) prevented the utilization of maltose. We also purified and characterized glucokinase and α-phosphoglucomutase, which catalyze early catabolic steps of the two carbohydrates. N. meningitidis catabolizes the two carbohydrates either via the Entner-Doudoroff (ED) pathway or the pentose phosphate pathway, thereby forming glycer...
Source: Journal of Molecular Microbiology and Biotechnology - July 25, 2016 Category: Microbiology Source Type: research

Clinical and Molecular Analysis of Klebsiella pneumoniae Causing Liver Abscess in China
Conclusions: Compared to the string test alone, integrating rmpA and abc detection with the string test is robuster and more extensive in the identification of liver abscess-causing hvKP strains. Multiplex PCR assays may therefore accelerate the early laboratory detection of hvKP. J Mol Microbiol Biotechnol 2016;26:245-251 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - July 21, 2016 Category: Microbiology Source Type: research