Journal of Molecular Microbiology and Biotechnology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

The Protein Expression Level of a Heterogeneous Gene Inserted in LIPI-1 of the Listeria ivanovii Genome Relies on Its Insertion Orientation
In this study, we inserted theM. tuberculosisantigen geneRv0129cinto LI strains at the same site as the genome but with a different insertion orientation. RT-qPCR and Western blot showed that when the insertion orientation of the heterogeneous gene was opposite to theLIorfXYZgene in the Listeriapathogenicity island 1 in the bacterial genome, the heterogeneous gene could be transcribed well but the protein expression level seemed limited, both in vitro and in vivo. When inserted at an orientation consistent withLIorfXYZ at the same site in the genome, the expected 43-kD protein was observed in vitro as well as in a mouse mo...
Source: Journal of Molecular Microbiology and Biotechnology - November 22, 2017 Category: Microbiology Source Type: research

The Protein Expression Level of a Heterogeneous Gene Inserted in LIPI-1 of the Listeria ivanovii Genome Relies on Its Insertion Orientation
In this study, we inserted theM. tuberculosisantigen geneRv0129cinto LI strains at the same site as the genome but with a different insertion orientation. RT-qPCR and Western blot showed that when the insertion orientation of the heterogeneous gene was opposite to theLIorfXYZgene in the Listeriapathogenicity island 1 in the bacterial genome, the heterogeneous gene could be transcribed well but the protein expression level seemed limited, both in vitro and in vivo. When inserted at an orientation consistent withLIorfXYZ at the same site in the genome, the expected 43-kD protein was observed in vitro as well as in a mouse mo...
Source: Journal of Molecular Microbiology and Biotechnology - November 21, 2017 Category: Microbiology Source Type: research

The Membrane Attack Complex/Perforin Superfamily
The membrane attack complex/perforin (MACPF) superfamily consists of a diverse group of proteins involved in bacterial pathogenesis and sporulation as well as eukaryotic immunity, embryonic development, neural migration and fruiting body formation. The present work shows that the evolutionary relationships between the members of the superfamily, previously suggested by comparison of their tertiary structures, can also be supported by analyses of their primary structures. The superfamily includes the MACPF family (TC 1.C.39), the cholesterol-dependent cytolysin (CDC) family (TC 1.C.12.1 and 1.C.12.2) and the pleurotolysin p...
Source: Journal of Molecular Microbiology and Biotechnology - November 16, 2017 Category: Microbiology Source Type: research

Progress in Bacillus subtilis Spore Surface Display Technology towards Environment, Vaccine Development, and Biocatalysis
Spore surface display is the most desirable with enhanced effects, low cost, less time consuming and the most promising technology for environmental, medical, and industrial development. Spores have various applications in industry due to their ability to survive in harsh industrial processes including heat resistance, alkaline tolerance, chemical tolerance, easy recovery, and reusability. Yeast and bacteria, including gram-positive and -negative, are the most frequently used organisms for the display of various proteins (eukaryotic and prokaryotic), but unlike spores, they can rupture easily due to nutritive properties, s...
Source: Journal of Molecular Microbiology and Biotechnology - October 25, 2017 Category: Microbiology Source Type: research

A Review of Conventional PCR Assays for the Detection of Selected Phytopathogens of Wheat
This article reviews frequently used DNA regions for fungus identification and discusses already known PCR assays for detection of the aforementioned wheat pathogens. We demonstrate that PCR-based wheat pathogen identification assays require further research. In particular, the number of diagnostic tests forFusarium graminearum,Puccinia spp., andP. tritici-repentis are insufficient.J Mol Microbiol Biotechnol 2017;27:175-189 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - October 25, 2017 Category: Microbiology Source Type: research

Antitumor Effect of a DNA Vaccine Harboring Prostate Cancer-Specific Antigen with IL-12 as an Intramolecular Adjuvant
To improve the lower immune intensity of DNA vaccines, we developed a DNA vaccine based on prostate cancer-specific antigen (PSA), which has been suggested as a potential target for prostate cancer therapy, and enhanced the DNA vaccine potency using interleukin-12 (IL-12) as an intramolecular adjuvant. A series of DNA plasmids encoding human PSA, IL-12, and their conjugates was constructed and injected into female mice intramuscularly, followed by an electric pulse. The humoral and cellular immune responses after immunization were detected by ELISA and ELISPOT, respectively. To evaluate the therapeutic efficacy of these pl...
Source: Journal of Molecular Microbiology and Biotechnology - October 25, 2017 Category: Microbiology Source Type: research

Heterologous Expression of Spinosyn Biosynthetic Gene Cluster in Streptomyces Species Is Dependent on the Expression of Rhamnose Biosynthesis Genes
This report demonstrates spinosyn production in 2Streptomyces strains and stresses the essential role of rhamnose in this process. This work also provides a potential alternative route for producing spinosyn analogs by means of genetic manipulation in the heterologous hosts.J Mol Microbiol Biotechnol 2017;27:190-198 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - October 25, 2017 Category: Microbiology Source Type: research

Influence of NanoLC Column and Gradient Length as well as MS/MS Frequency and Sample Complexity on Shotgun Protein Identification of Marine Bacteria
Protein identification by shotgun proteomics, i.e., nano-liquid chromatography (nanoLC) peptide separation online coupled to electrospray ionization (ESI) mass spectrometry (MS)/MS, is the most widely used gel-free approach in proteome research. While the mass spectrometer accounts for mass accuracy and MS/MS frequency, the nanoLC setup and gradient time influence the number of peptides available for MS analysis, which ultimately determine the number of proteins identifiable. Here, we report on the influence of (i) analytical column length (15, 25, or 50 cm) coupled to (ii) the applied gradient length (120, 240, 360, 480, ...
Source: Journal of Molecular Microbiology and Biotechnology - October 25, 2017 Category: Microbiology Source Type: research

Overexpression of a Laccase with Dye Decolorization Activity from Bacillus sp. Induced in Escherichia coli
In this study, a novel laccase gene was cloned from Bacillus sp., analyzed, and functionally expressed in Escherichia coli. The laccase was highly induced in theE. coli expression system with a maximum intracellular activity of 16 U mg-1 protein. The optimal temperature and pH of the purified laccase were 40 °C and 4.6, respectively, when ABTS (2,2'-azino-bis[3-ethylbenzothiazoline-6-sulfonate]) was used as the substrate. The purified laccase showed high stability in the pH range of 3.0-9.0, and retained more than 70% of its activity after 24 h of incubation at 40°C with a pH value of 9.0. Furthermore , the enzyme exhibi...
Source: Journal of Molecular Microbiology and Biotechnology - October 25, 2017 Category: Microbiology Source Type: research

Rare Human Codons and HCMV Translational Regulation
Restriction of protein synthesis characterizes human cytomegalovirus (HCMV) latency in the human host. In analyzing the molecular factors that hinder HCMV expression, the present study shows that HCMV genes frequently use 6 rare codons, i.e., GCG (Ala), CCG (Pro), CGT (Arg), CGC (Arg), TCG (Ser), and ACG (Thr). In some instances, the rare host codons are clustered along viral nucleotide sequences and represent the majority in sequences encoding short alanine and proline repeats. Given the positive correlation between codon usage, tRNA content, and protein production, the results support the hypothesis that HCMV usage of ra...
Source: Journal of Molecular Microbiology and Biotechnology - October 25, 2017 Category: Microbiology Source Type: research

Structure-Function Analysis of the Periplasmic Escherichia coli Cyclophilin PpiA in Relation to Biofilm Formation
The presence of peptidyl-prolylcis/trans isomerases (PPIases, EC: 5.2.1.8) in all domains of life indicates their biological importance. Cyclophilin PpiA, present in the periplasm of gram-negative bacteria, possesses PPIase activity but its physiological functionsare still not clearly defined. Here, we demonstrate that the #x0394;ppiA deletion strain fromEscherichia coli exhibits an increased ability for biofilm formation and enhanced swimming motility compared to the wild-type strain. To identify structural features of PpiA which are necessary for the negative modulation of biofilm formation, we constructed a series of mu...
Source: Journal of Molecular Microbiology and Biotechnology - October 25, 2017 Category: Microbiology Source Type: research

ERAP1 and PDE8A Are Downregulated in Cattle Protected against Bovine Tuberculosis
In this study, we found thatERAP1 andPDE8A were downregulated in vaccinated cattle that were protected from experimentalM. bovis challenge. Remarkably, both genes encode proteins that have been negatively associated with immune protection against bTB.J Mol Microbiol Biotechnol 2017;27:237-245 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - October 25, 2017 Category: Microbiology Source Type: research

Cell Wall-Treated Lactococcus lactis Increases the Plasmid Transfer Efficiency of Internal Ribosome Entry Site-Incorporated Lactococcal Bicistronic Vector into DF1 Cells
This study demonstrates that cell wall treatment ofLactococcus lactis harbouring the internal ribosome entry site-incorporated lactococcal bicistronic vector pNZ:VIG mediated the delivery of genes into an eukaryotic cell line, DF1 cells, through bactofection. Bactofection analysis showed that the pNZ:VIG plasmid inL. lactis can be transferred into DF1 cells and that both theVP2 andgfp genes cloned in the plasmid can be transcribed and translated. The protein band relative to the Mr of VP2 protein (49 kDa) was successfully detected via Western blot analysis, while green fluorescence was successfully detected using a fluores...
Source: Journal of Molecular Microbiology and Biotechnology - October 25, 2017 Category: Microbiology Source Type: research

Cell Wall-Treated Lactococcus lactis Increases the Plasmid Transfer Efficiency of Internal Ribosome Entry Site-Incorporated Lactococcal Bicistronic Vector into DF1 Cells
This study demonstrates that cell wall treatment ofLactococcus lactis harbouring the internal ribosome entry site-incorporated lactococcal bicistronic vector pNZ:VIG mediated the delivery of genes into an eukaryotic cell line, DF1 cells, through bactofection. Bactofection analysis showed that the pNZ:VIG plasmid inL. lactis can be transferred into DF1 cells and that both theVP2 andgfp genes cloned in the plasmid can be transcribed and translated. The protein band relative to the Mr of VP2 protein (49 kDa) was successfully detected via Western blot analysis, while green fluorescence was successfully detected using a fluores...
Source: Journal of Molecular Microbiology and Biotechnology - October 20, 2017 Category: Microbiology Source Type: research