Journal of Molecular Microbiology and Biotechnology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

ERAP1 and PDE8A Are Downregulated in Cattle Protected against Bovine Tuberculosis
In this study, we found thatERAP1 andPDE8A were downregulated in vaccinated cattle that were protected from experimentalM. bovis challenge. Remarkably, both genes encode proteins that have been negatively associated with immune protection against bTB.J Mol Microbiol Biotechnol 2017;27:237-245 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - September 14, 2017 Category: Microbiology Source Type: research

 ERAP1 and PDE8A Are Downregulated in Cattle Protected against Bovine Tuberculosis
In this study, we found thatERAP1 andPDE8A were downregulated in vaccinated cattle that were protected from experimentalM. bovis challenge. Remarkably, both genes encode proteins that have been negatively associated with immune protection against bTB.J Mol Microbiol Biotechnol 2017;27:237-245 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - September 13, 2017 Category: Microbiology Source Type: research

Expression and Characterization of Serotype 2 Streptococcus suis Arginine Deiminase
Conclusions: This study profoundly demonstrated the involvement of ArcA enzymatic activity inS. suis survival under acidic conditions.J Mol Microbiol Biotechnol 2017;27:133-146 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - September 12, 2017 Category: Microbiology Source Type: research

Structure-Function Analysis of the Periplasmic Escherichia coli Cyclophilin PpiA in Relation to Biofilm Formation
The presence of peptidyl-prolylcis/trans isomerases (PPIases, EC: 5.2.1.8) in all domains of life indicates their biological importance. Cyclophilin PpiA, present in the periplasm of gram-negative bacteria, possesses PPIase activity but its physiological functionsare still not clearly defined. Here, we demonstrate that the #x0394;ppiA deletion strain fromEscherichia coli exhibits an increased ability for biofilm formation and enhanced swimming motility compared to the wild-type strain. To identify structural features of PpiA which are necessary for the negative modulation of biofilm formation, we constructed a series of mu...
Source: Journal of Molecular Microbiology and Biotechnology - September 11, 2017 Category: Microbiology Source Type: research

Structure-Function Analysis of the Periplasmic Escherichia coli Cyclophilin PpiA in Relation to Biofilm Formation
The presence of peptidyl-prolylcis/trans isomerases (PPIases, EC: 5.2.1.8) in all domains of life indicates their biological importance. Cyclophilin PpiA, present in the periplasm of gram-negative bacteria, possesses PPIase activity but its physiological functionsare still not clearly defined. Here, we demonstrate that the #x0394;ppiA deletion strain fromEscherichia coli exhibits an increased ability for biofilm formation and enhanced swimming motility compared to the wild-type strain. To identify structural features of PpiA which are necessary for the negative modulation of biofilm formation, we constructed a series of mu...
Source: Journal of Molecular Microbiology and Biotechnology - September 8, 2017 Category: Microbiology Source Type: research

Overexpression of a Laccase with Dye Decolorization Activity from Bacillus sp. Induced in Escherichia coli
In this study, a novel laccase gene was cloned from Bacillus sp., analyzed, and functionally expressed in Escherichia coli. The laccase was highly induced in theE. coli expression system with a maximum intracellular activity of 16 U mg-1 protein. The optimal temperature and pH of the purified laccase were 40 °C and 4.6, respectively, when ABTS (2,2'-azino-bis[3-ethylbenzothiazoline-6-sulfonate]) was used as the substrate. The purified laccase showed high stability in the pH range of 3.0-9.0, and retained more than 70% of its activity after 24 h of incubation at 40°C with a pH value of 9.0. Furthermore , the enzyme exhibi...
Source: Journal of Molecular Microbiology and Biotechnology - September 1, 2017 Category: Microbiology Source Type: research

Overexpression of a Laccase with Dye Decolorization Activity from Bacillus sp. Induced in Escherichia coli 
In this study, a novel laccase gene was cloned from Bacillus sp., analyzed, and functionally expressed in Escherichia coli. The laccase was highly induced in theE. coli expression system with a maximum intracellular activity of 16 U mg-1 protein. The optimal temperature and pH of the purified laccase were 40 °C and 4.6, respectively, when ABTS (2,2'-azino-bis[3-ethylbenzothiazoline-6-sulfonate]) was used as the substrate. The purified laccase showed high stability in the pH range of 3.0-9.0, and retained more than 70% of its activity after 24 h of incubation at 40°C with a pH value of 9.0. Furthermore , the enzyme exhibi...
Source: Journal of Molecular Microbiology and Biotechnology - August 31, 2017 Category: Microbiology Source Type: research

Rare Human Codons and HCMV Translational Regulation
Restriction of protein synthesis characterizes human cytomegalovirus (HCMV) latency in the human host. In analyzing the molecular factors that hinder HCMV expression, the present study shows that HCMV genes frequently use 6 rare codons, i.e., GCG (Ala), CCG (Pro), CGT (Arg), CGC (Arg), TCG (Ser), and ACG (Thr). In some instances, the rare host codons are clustered along viral nucleotide sequences and represent the majority in sequences encoding short alanine and proline repeats. Given the positive correlation between codon usage, tRNA content, and protein production, the results support the hypothesis that HCMV usage of ra...
Source: Journal of Molecular Microbiology and Biotechnology - August 31, 2017 Category: Microbiology Source Type: research

Influence of NanoLC Column and Gradient Length as well as MS/MS Frequency and Sample Complexity on Shotgun Protein Identification of Marine Bacteria
Protein identification by shotgun proteomics, i.e., nano-liquid chromatography (nanoLC) peptide separation online coupled to electrospray ionization (ESI) mass spectrometry (MS)/MS, is the most widely used gel-free approach in proteome research. While the mass spectrometer accounts for mass accuracy and MS/MS frequency, the nanoLC setup and gradient time influence the number of peptides available for MS analysis, which ultimately determine the number of proteins identifiable. Here, we report on the influence of (i) analytical column length (15, 25, or 50 cm) coupled to (ii) the applied gradient length (120, 240, 360, 480, ...
Source: Journal of Molecular Microbiology and Biotechnology - August 29, 2017 Category: Microbiology Source Type: research

Heterologous Expression of Spinosyn Biosynthetic Gene Cluster in Streptomyces Species Is Dependent on the Expression of Rhamnose Biosynthesis Genes
This report demonstrates spinosyn production in 2Streptomyces strains and stresses the essential role of rhamnose in this process. This work also provides a potential alternative route for producing spinosyn analogs by means of genetic manipulation in the heterologous hosts.J Mol Microbiol Biotechnol 2017;27:190-198 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - August 21, 2017 Category: Microbiology Source Type: research

Heterologous Expression of Spinosyn Biosynthetic Gene Cluster in Streptomyces Species Is Dependent on the Expression of Rhamnose Biosynthesis Genes
This report demonstrates spinosyn production in 2Streptomyces strains and stresses the essential role of rhamnose in this process. This work also provides a potential alternative route for producing spinosyn analogs by means of genetic manipulation in the heterologous hosts.J Mol Microbiol Biotechnol 2017;27:190-198 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - August 18, 2017 Category: Microbiology Source Type: research

A Review of Conventional PCR Assays for the Detection of Selected Phytopathogens of Wheat
This article reviews frequently used DNA regions for fungus identification and discusses already known PCR assays for detection of the aforementioned wheat pathogens. We demonstrate that PCR-based wheat pathogen identification assays require further research. In particular, the number of diagnostic tests forFusarium graminearum,Puccinia spp., andP. tritici-repentis are insufficient.J Mol Microbiol Biotechnol 2017;27:175-189 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - August 3, 2017 Category: Microbiology Source Type: research

Antitumor Effect of a DNA Vaccine Harboring Prostate Cancer-Specific Antigen with IL-12 as an Intramolecular Adjuvant
To improve the lower immune intensity of DNA vaccines, we developed a DNA vaccine based on prostate cancer-specific antigen (PSA), which has been suggested as a potential target for prostate cancer therapy, and enhanced the DNA vaccine potency using interleukin-12 (IL-12) as an intramolecular adjuvant. A series of DNA plasmids encoding human PSA, IL-12, and their conjugates was constructed and injected into female mice intramuscularly, followed by an electric pulse. The humoral and cellular immune responses after immunization were detected by ELISA and ELISPOT, respectively. To evaluate the therapeutic efficacy of these pl...
Source: Journal of Molecular Microbiology and Biotechnology - August 3, 2017 Category: Microbiology Source Type: research

Progress in Bacillus subtilis Spore Surface Display Technology towards Environment, Vaccine Development, and Biocatalysis
Spore surface display is the most desirable with enhanced effects, low cost, less time consuming and the most promising technology for environmental, medical, and industrial development. Spores have various applications in industry due to their ability to survive in harsh industrial processes including heat resistance, alkaline tolerance, chemical tolerance, easy recovery, and reusability. Yeast and bacteria, including gram-positive and -negative, are the most frequently used organisms for the display of various proteins (eukaryotic and prokaryotic), but unlike spores, they can rupture easily due to nutritive properties, s...
Source: Journal of Molecular Microbiology and Biotechnology - June 13, 2017 Category: Microbiology Source Type: research

Progress in Bacillus subtilis Spore Surface Display Technology towards Environment, Vaccine Development, and Biocatalysis
Spore surface display is the most desirable with enhanced effects, low cost, less time consuming and the most promising technology for environmental, medical, and industrial development. Spores have various applications in industry due to their ability to survive in harsh industrial processes including heat resistance, alkaline tolerance, chemical tolerance, easy recovery, and reusability. Yeast and bacteria, including gram-positive and -negative, are the most frequently used organisms for the display of various proteins (eukaryotic and prokaryotic), but unlike spores, they can rupture easily due to nutritive properties, s...
Source: Journal of Molecular Microbiology and Biotechnology - June 12, 2017 Category: Microbiology Source Type: research