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Influences of Various Peptide Linkers on the Thermotoga maritima MSB8 Nitrilase Displayed on the Spore Surface of Bacillus subtilis 
In the present study, fusion genes composed ofThermotoga maritima MSB8 nitrilase andBacillus subtilis 168 outer coat protein CotG were constructed with various peptide linkers and displayed onB. subtilis DB 403 spores. The successful display of CotG-nit fusion proteins on the spore surface ofB. subtilis was verified by Western blot analysis and activity measurement. It was demonstrated that the fusion with linker GGGGSEAAAKGGGGS presented the highest thermal and pH stability, which is 2.67- and 1.9-fold of the fusion without linker. In addition, fusion with flexible linker (GGGGS)3 demonstrated better thermal and pH stabil...
Source: Journal of Molecular Microbiology and Biotechnology - January 19, 2017 Category: Microbiology Source Type: research

Production and Rheological Properties of Welan Gum Produced by Sphingomonas sp. ATCC 31555 with Different Nitrogen Sources
This study aimed to investigate the effect of nitrogen sources on the production and rheological properties of welan gum produced bySphingomonas sp. ATCC 31555. Six different nitrogen sources were used for ATCC 31555 fermentation, and 2 of these were further analyzed due to their more positive influence on welan gum production and bacterial biomass. Bacterial biomass, welan gum yield, welan viscosity, molecular weight, monosaccharide composition, acyl content, and welan structure were analyzed. Welan gum production and the biomass concentration of ATCC 31555 were higher in media containing NaNO3 and beef extract. Welan vis...
Source: Journal of Molecular Microbiology and Biotechnology - January 17, 2017 Category: Microbiology Source Type: research

Complete Sequence and Organization of pFR260, the Bacillus thuringiensis INTA Fr7-4 Plasmid Harboring Insecticidal Genes
We report the complete sequence and analysis of pFR260, a novel megaplasmid of 260,595 bp from theBacillus thuringiensis strain INTA Fr7-4 isolated in Argentina. It carries 7 insecticidal genes: 3cry8 copies previously reported, 2vip1, and 2vip2. Also, it carries a gene encoding a putative atypical Cry protein. These genes are arranged in a region of approximately 105 kbp in size with characteristics of a pathogenicity island with a potential coleopteran-specific insecticide profile. DNA strand composition asymmetry, as determined by GC skew analysis, and the presence of a Rep protein involved in the initiation of replicat...
Source: Journal of Molecular Microbiology and Biotechnology - January 17, 2017 Category: Microbiology Source Type: research

Production and Rheological Properties of Welan Gum Produced by Sphingomonas sp. ATCC 31555 with Different Nitrogen Sources
This study aimed to investigate the effect of nitrogen sources on the production and rheological properties of welan gum produced bySphingomonas sp. ATCC 31555. Six different nitrogen sources were used for ATCC 31555 fermentation, and 2 of these were further analyzed due to their more positive influence on welan gum production and bacterial biomass. Bacterial biomass, welan gum yield, welan viscosity, molecular weight, monosaccharide composition, acyl content, and welan structure were analyzed. Welan gum production and the biomass concentration of ATCC 31555 were higher in media containing NaNO3 and beef extract. Welan vis...
Source: Journal of Molecular Microbiology and Biotechnology - January 16, 2017 Category: Microbiology Source Type: research

Complete Sequence and Organization of pFR260, the Bacillus thuringiensis INTA Fr7-4 Plasmid Harboring Insecticidal Genes
We report the complete sequence and analysis of pFR260, a novel megaplasmid of 260,595 bp from theBacillus thuringiensis strain INTA Fr7-4 isolated in Argentina. It carries 7 insecticidal genes: 3cry8 copies previously reported, 2vip1, and 2vip2. Also, it carries a gene encoding a putative atypical Cry protein. These genes are arranged in a region of approximately 105 kbp in size with characteristics of a pathogenicity island with a potential coleopteran-specific insecticide profile. DNA strand composition asymmetry, as determined by GC skew analysis, and the presence of a Rep protein involved in the initiation of replicat...
Source: Journal of Molecular Microbiology and Biotechnology - January 16, 2017 Category: Microbiology Source Type: research

Antipathy of Trichoderma against Sclerotium rolfsii Sacc.: Evaluation of Cell Wall-Degrading Enzymatic Activities and Molecular Diversity Analysis of Antagonists
The fungusTrichoderma is a teleomorph of theHypocrea genus and associated with biological control of plant diseases. The microscopic, biochemical, and molecular characterization ofTrichodermawas carried out and evaluated for in vitro antagonistic activity against the fungal pathogenSclerotium rolfsii causing stem rot disease in groundnut. In total, 11 isolates ofTrichoderma were examined for antagonism at 6 and 12 days after inoculation (DAI). Out of 11,T. virensNBAII Tvs12 evidenced the highest (87.91%) growth inhibition of the test pathogen followed byT. koningii MTCC 796 (67.03%),T. virideNBAII Tv23 (63.74%), andT. harz...
Source: Journal of Molecular Microbiology and Biotechnology - January 13, 2017 Category: Microbiology Source Type: research

Antipathy of Trichoderma against Sclerotium rolfsii Sacc.: Evaluation of Cell Wall-Degrading Enzymatic Activities and Molecular Diversity Analysis of Antagonists
The fungusTrichoderma is a teleomorph of theHypocrea genus and associated with biological control of plant diseases. The microscopic, biochemical, and molecular characterization ofTrichodermawas carried out and evaluated for in vitro antagonistic activity against the fungal pathogenSclerotium rolfsii causing stem rot disease in groundnut. In total, 11 isolates ofTrichoderma were examined for antagonism at 6 and 12 days after inoculation (DAI). Out of 11,T. virensNBAII Tvs12 evidenced the highest (87.91%) growth inhibition of the test pathogen followed byT. koningii MTCC 796 (67.03%),T. virideNBAII Tv23 (63.74%), andT. harz...
Source: Journal of Molecular Microbiology and Biotechnology - January 12, 2017 Category: Microbiology Source Type: research

The Role of the ydiB Gene, Which Encodes Quinate/Shikimate Dehydrogenase, in the Production of Quinic, Dehydroshikimic and Shikimic Acids in a PTS- Strain of Escherichia coli
We report the effect of the inactivation or the overexpression ofydiB inE. coli strain PB12.SA22 on SA, QA, and DHS production in batch fermentor cultures. The results showed that the inactivation ofydiBresulted in a 75% decrease in the molar yield of QA and a 6.17% reduction in the yield of QA (mol/mol) relative to SA with respect to the parental strain. The overexpression ofydiB caused a 500% increase in the molar yield of QA and resulted in a 152% increase in QA (mol/mol) relative to SA, with a sharp decrease in SA production. Production of SA, QA, and DHS in parental and derivativeydiBstrains suggests that the synthesi...
Source: Journal of Molecular Microbiology and Biotechnology - November 18, 2016 Category: Microbiology Source Type: research

The Role of the ydiB Gene, Which Encodes Quinate/Shikimate Dehydrogenase, in the Production of Quinic, Dehydroshikimic and Shikimic Acids in a PTS - Strain of Escherichia coli 
We report the effect of the inactivation or the overexpression ofydiB inE. coli strain PB12.SA22 on SA, QA, and DHS production in batch fermentor cultures. The results showed that the inactivation ofydiBresulted in a 75% decrease in the molar yield of QA and a 6.17% reduction in the yield of QA (mol/mol) relative to SA with respect to the parental strain. The overexpression ofydiB caused a 500% increase in the molar yield of QA and resulted in a 152% increase in QA (mol/mol) relative to SA, with a sharp decrease in SA production. Production of SA, QA, and DHS in parental and derivativeydiBstrains suggests that the synthesi...
Source: Journal of Molecular Microbiology and Biotechnology - November 17, 2016 Category: Microbiology Source Type: research

A Codon Deletion at the Beginning of Green Fluorescent Protein Genes Enhances Protein Expression
Recombinant protein expression is one of the key issues in protein engineering and biotechnology. Among the different models for assessing protein production and structure-function studies, green fluorescent protein (GFP) is one of the preferred models because of its importance as a reporter in cellular and molecular studies. In this research we analyze the effect of codon deletions near the amino terminus of different GFP proteins on fluorescence. Our study includes Gly4 deletions in the enhanced GFP (EGFP), the red-shifted GFP and the red-shifted EGFP. The Gly4 deletion mutants and their corresponding wild-type counterpa...
Source: Journal of Molecular Microbiology and Biotechnology - November 7, 2016 Category: Microbiology Source Type: research

Contents Vol. 26, 2016
J Mol Microbiol Biotechnol 2016;26:I-IV (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - October 25, 2016 Category: Microbiology Source Type: research

HA1-2-fljB Vaccine Induces Immune Responses against Pandemic Swine-Origin H1N1 Influenza Virus in Mice
In 2009, a novel pandemic swine-origin influenza A (H1N1) virus caused a public emergency of international concern. Vaccination is the primary strategy for the control of influenza epidemics. However, the poor immunopotency of many vaccine antigens is a major barrier to the development of effective vaccines against influenza. Flagellin, a Toll-like receptor 5 (TLR5) ligand, has been used as an adjuvant to enhance the immunopotency of vaccines in preclinical studies. Here, we developed a recombinant candidate vaccine, HA1-2-fljB, in which the globular head of the hemagglutinin (HA) antigen (residues 62-284) from H1N1 virus ...
Source: Journal of Molecular Microbiology and Biotechnology - October 7, 2016 Category: Microbiology Source Type: research

Molecular Epidemiology of Human Metapneumovirus in Riyadh Province, Saudi Arabia
Human metapneumovirus (HMPV) is an important cause of respiratory tract illness in children. Two HMPV subgroups, A and B, and four genotypes, A1, A2, B1 and B2, have been identified. Concurrent circulation of the different genotypes in yearly epidemics has been recorded globally, but not in Saudi Arabia. The current report was designed to study HMPV epidemiology in Saudi children and to analyze the genetic diversity and circulation patterns. Nasopharyngeal aspirates (n = 174) were collected from hospitalized children in Riyadh (2008-2009). The screening of samples using real-time RT-PCR identified 19 HMPV strains. The majo...
Source: Journal of Molecular Microbiology and Biotechnology - September 30, 2016 Category: Microbiology Source Type: research

Evidence of Interruption of the comM Gene in a Large Series of Clinical Isolates of Multidrug-Resistant Acinetobacter baumannii
This study demonstrated that thecomM gene is targeted by transposons in Iranian MDR-AB isolates belonging to different GCs. The data also showed that the carriage of interruptedcomM is not exclusive to MDR isolates ofA. baumannii.J Mol Microbiol Biotechnol 2016;26:410-413 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - September 16, 2016 Category: Microbiology Source Type: research

Proteomic Analyses Uncover the Mechanisms Underlying Antibiotic Resistance Differences among Three Acinetobacter baumannii Isolates
This study aimed to investigate the molecular mechanisms underlying the antibiotic resistance difference among threeAcinetobacter baumannii isolates. FiftyA. baumannii isolates were first subjected to an antimicrobial susceptibility test, then three isolates differing in antibiotic resistance were selected and subjected to iTRAQ (isobaric tags for relative and absolute quantification)-based proteomics analysis. Differential proteins among the threeA. baumannii isolates were further identified and subjected to gene ontology functional enrichment analysis. A resistant isolate (A1), a less resistant one (A8) and a susceptible...
Source: Journal of Molecular Microbiology and Biotechnology - September 16, 2016 Category: Microbiology Source Type: research