Journal of Molecular Microbiology and Biotechnology 
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This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.Heterologous Expression of 3-O-Deacylase in Acinetobacter baumannii Modulates the Endotoxicity of Lipopolysaccharide
The lipopolysaccharide (LPS) of Acinetobacter baumannii is a potent stimulator of proinflammatory cytokines, such as interleukin-6 (IL-6). The 3-O-deacylase (PagL)-modifying enzyme that removes the 3-O-linked acyl chain from the disaccharide backbone of lipid A provides the opportunity to develop a new therapeutic compound that could reduce detrimental inflammatory responses. The plasmid pMMB66EH-PagL obtained by recombinant DNA technology was electroporated into A. baumannii ATCC 19606. Compared with wild-type LPS, outer membrane vesicles and inactivated whole cells of engineered bacteria had a statistically significant d...
Source: Journal of Molecular Microbiology and Biotechnology - February 13, 2015 Category: Microbiology Source Type: research
Evaluation of a Possible Role of Stigmatella aurantiaca ACE in Aβ Peptide Degradation: A Molecular Modeling Approach
Amyloid-β (Aβ)-degrading enzymes are known to degrade Aβ peptides, a causative agent of Alzheimer's disease. These enzymes are responsible for maintaining Aβ concentration. However, loss of such enzymes or their Aβ-degrading activity because of certain genetic as well as nongenetic reasons initiates the accumulation of Aβ peptides in the human brain. Considering the limitations of the human enzymes in clearing Aβ peptide, the search for microbial enzymes that could cleave Aβ is necessary. Hence, we built a three-dimensional model of angiotensin-converting enzyme (ACE) from Stigmatella aurantiaca using homology mode...
Source: Journal of Molecular Microbiology and Biotechnology - February 10, 2015 Category: Microbiology Source Type: research
Biochemical Characterization and Antioxidant and Antiproliferative Activities of Different Ganoderma Collections
This study provides a comparison between G. lucidum and G. resinaceum mycelial strains, and shows that G. resinaceum could be utilized to obtain several bioactive compounds.J Mol Microbiol Biotechnol 2015;25:16-25 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - February 3, 2015 Category: Microbiology Source Type: research
Lactic Acid Bacteria as a Surface Display Platform for Campylobacter jejuni Antigens
Conclusion: Our results documented that the LysM-mediated binding system allows us to construct GEM particles that present 2 C. jejuni antigens.J Mol Microbiol Biotechnol 2015;25:1-10 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - February 3, 2015 Category: Microbiology Source Type: research
Prevalence and Genotyping of Commensal Neisseria with Reduced Susceptibility to Penicillin
We analyzed 85 Neisseria spp. strains collected by swabbing from neutropenic patients to determine the prevalence of reduced susceptibility to penicillin and to ascertain the clonal relationship between these strains. High genetic diversity and an elevated level of penicillin resistance were found among commensal Neisseria clinical isolates.J Mol Microbiol Biotechnol 2015;25:11-15 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - February 3, 2015 Category: Microbiology Source Type: research
On the Possibility of Lipid-Induced Regulation of Conformation and Immunogenicity of Influenza A Virus H1/N1 Hemagglutinin as Antigen of TI-Complexes
The tubular immunostimulating complex (TI-complex) consisting of cucumarioside A2-2, cholesterol and monogalactosyldiacylglycerol (MGDG) from marine macrophytes is the perspective antigen delivery system for subunit vaccines. MGDG is a lipid matrix for the protein antigen incorporated in the TI-complex. The aim of the present work was to study the influence of MGDGs from different macrophytes on conformation and immunogenicity of the secreted recombinant uncleaved hemagglutinin monomer (HA0S) of influenza A virus H1/N1. Differential scanning calorimetry, fluorescence spectroscopy and circular dichroism showed a dependence ...
Source: Journal of Molecular Microbiology and Biotechnology - November 7, 2014 Category: Microbiology Source Type: research
In vitro and in vivo Activity of the Dimer of PMAP-36 Expressed in Pichia pastoris
The antimicrobial peptide PMAP-36 exists as a homodimer stabilized by an intermolecular disulfide bridge. The dimer of PMAP-36 exhibits a potent and rapid microbicidal activity against a wide spectrum of microorganisms. The gene encoding the antiparallel dimer (PMAP-36)2 was designed and codon-optimized according to bias of Pichia pastoris. The gene was then expressed in the P. pastoris strain GS115. The concentration of the recombinant product reached 106 mg/l. In vitro activity assays indicated that the recombinant peptide showed antimicrobial activities against Gram-positive and Gram-negative bacteria but did not cause ...
Source: Journal of Molecular Microbiology and Biotechnology - November 7, 2014 Category: Microbiology Source Type: research
Regression of Solid Tumors by Induction of MazF, a Bacterial mRNA Endoribonuclease
MazF from Escherichia coli is an endoribonuclease that specifically cleaves mRNAs at ACA sequences. Its induction in mammalian cells has been shown to cause programmed cell death. Here we explored if a bacterial MazF-MazE toxin-antitoxin system can be used for gene therapy. For this, we first constructed a tetracycline-inducible MazF expression system in human embryonic kidney cells (T-Rex 293-mazF). Solid tumors were formed by injecting T-Rex 293-mazF cells into nude mice. All 8 mice injected with the cells developed solid tumors, which regressed upon induction of MazF. In 4 mice, tumors completely regressed, while in the...
Source: Journal of Molecular Microbiology and Biotechnology - November 7, 2014 Category: Microbiology Source Type: research
Recent Approaches in Vaccine Development against Streptococcus pneumoniae
Streptococcus pneumoniae is a major cause of morbidity and mortality among children under 5 years of age worldwide. Vaccines have long been used for protection against pneumococcal infections. Capsular polysaccharides of pneumococci are main antigenic components of these vaccines. However, pneumococcal polysaccharide-based vaccines are not able to elicit appropriate immunological responses in young children and cannot induce the immune memory. Thus, pneumococcal conjugate vaccines were developed through chemical coupling of an immunogenic carrier protein to the capsule. The currently available pneumococcal conjugate vaccin...
Source: Journal of Molecular Microbiology and Biotechnology - November 7, 2014 Category: Microbiology Source Type: research
Psychobiotics and Their Involvement in Mental Health
J Mol Microbiol Biotechnol 2014;24:211-214 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - November 7, 2014 Category: Microbiology Source Type: research
Sequence and Expression of Two cry8 Genes from Bacillus thuringiensis INTA Fr7-4, a Native Strain from Argentina
We found and characterized two cry8 genes from the Bacillus thuringiensis strain INTA Fr7-4 isolated in Argentina. These genes, cry8Kb3 and cry8Pa3, are located in a tandem array within a 13,200-bp DNA segment sequenced from a preparation of total DNA. They encode 1,169- and 1,176-amino-acid proteins, respectively. Both genes were cloned with their promoter sequences and the proteins were expressed separately in an acrystalliferous strain of B. thuringiensis leading to the formation of ovoid crystals in the recombinant strains. The toxicity against larvae of Anthonomus grandis Bh. (Coleoptera: Curculionidae) of a spore-cry...
Source: Journal of Molecular Microbiology and Biotechnology - November 7, 2014 Category: Microbiology Source Type: research
Metagenome of Mehsani Buffalo Rumen Microbiota: An Assessment of Variation in Feed-Dependent Phylogenetic and Functional Classification
Conclusion: The present study provides a base to understand the modulating behavior of microbiota which can be manipulated to improve livestock nutrient utilization efficiency and for targeting the efficient catabolism of complex carbohydrate molecules as well.J Mol Microbiol Biotechnol 2014;24:249-261 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - November 7, 2014 Category: Microbiology Source Type: research
Molecular Characterization of Clinical Isolates of Moraxella catarrhalis by Randomly Amplified Polymorphic DNA Fingerprinting
Moraxella catarrhalis, a less virulent microorganism that colonizes the upper respiratory tract, has recently been associated with lower respiratory disease, especially in HIV-positive immunocompromised individuals and children. Here, we correlated the DNA clustering pattern of 24 clinical isolates of M. catarrhalis for β-lactamase production and drug resistance, from different disease groups using three different arbitrarily selected primers, P1 (5′-TCACGATGCA-3′), P14 (5′-GATCAAGTCC-3′) and P17 (5′-GATCTGACAC-3′). M. catarrhalis revealed three distinct banding patterns with primer P1, four with P14 and P17. ...
Source: Journal of Molecular Microbiology and Biotechnology - November 7, 2014 Category: Microbiology Source Type: research
Rational Design of K173A Substitution Enhances Thermostability Coupled with Catalytic Activity of Enterobacter sp. Bn12 Lipase
In conclusion, results of this study demonstrate the positive role of generation of a stabilizing protein motif through rational protein engineering that improves the enzyme characteristics.J Mol Microbiol Biotechnol 2014;24:262-269 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - November 7, 2014 Category: Microbiology Source Type: research
Dissemination of ESBL-Producing Escherichia coli of Chicken Origin to the Nearby River Water
In this study, antimicrobial resistance, resistance genes, and genetic similarity of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli of different origins (chicken feces and upstream and downstream river waters) were analyzed to track the spread of drug-resistant bacteria of animals. The results showed that a total of 29 ESBL-producing E. coli were obtained from 258 samples, and isolation rates of the ESBL-producing E. coli from chicken feces and upstream and downstream waters were 10.7% (16/150), 3.7% (1/27), and 14.8% (12/81), respectively. The ESBL-producing E. coli from upstream water was resistant to 7...
Source: Journal of Molecular Microbiology and Biotechnology - November 7, 2014 Category: Microbiology Source Type: research
