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Genomics Perspectives on Metabolism, Survival Strategies, and Biotechnological Applications of Brettanomyces bruxellensis LAMAP2480
In conclusion, the analysis of the genome ofB. bruxellensis LAMAP2480 reaffirms the biotechnological potential of this strain. This research represents an interesting platform for the study of the spoilage yeastB. bruxellensis.J Mol Microbiol Biotechnol 2017;27:147-158 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - June 9, 2017 Category: Microbiology Source Type: research

Genomics Perspectives on Metabolism, Survival Strategies, and Biotechnological Applications of Brettanomyces bruxellensis LAMAP2480
In conclusion, the analysis of the genome ofB. bruxellensis LAMAP2480 reaffirms the biotechnological potential of this strain. This research represents an interesting platform for the study of the spoilage yeastB. bruxellensis.J Mol Microbiol Biotechnol 2017;27:147-158 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - June 8, 2017 Category: Microbiology Source Type: research

Expression and Characterization of Serotype 2 Streptococcus suis Arginine Deiminase
Conclusions: This study profoundly demonstrated the involvement of ArcA enzymatic activity inS. suis survival under acidic conditions.J Mol Microbiol Biotechnol 2017;27:133-146 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - April 28, 2017 Category: Microbiology Source Type: research

Science, Innovation and the Future of Humanity
J Mol Microbiol Biotechnol 2017;27:128-132 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - April 27, 2017 Category: Microbiology Source Type: research

Synthetic, Switchable Enzymes
The construction of switchable, radiation-controlled, aptameric enzymes - “swenzymes ” - is, in principle, feasible. We propose a strategy to make such catalysts from 2 (or more) aptamers each selected to bind specifically to one of the substrates in, for example, a 2-substrate reaction. Construction of a combinatorial library of candidate swenzymes entails selecting a set of a million aptamers that bind one substrate and a second set of a million aptamers that bind the second substrate; the aptamers in these sets are then linked pairwise by a linker, thus bringing together the substrates. In the presence of the substr...
Source: Journal of Molecular Microbiology and Biotechnology - April 27, 2017 Category: Microbiology Source Type: research

Purification and Biochemical and Kinetic Properties of an Endo-Polygalacturonase from the Industrial Fungus Aspergillus sojae 
An endo-polygalacturonase secreted byAspergillus sojaewas characterized after being purified to homogeneity from submerged cultures with orange peel as the sole carbon source by gel filtration and ion-exchange chromatographies. According to SDS-PAGE and analytical isoelectric focusing analyses, the enzyme presents a molecular weight of 47 kDa and pI value of 4.2. This enzyme exhibits considerable stability under highly acidic to neutral conditions (pH 1.5-6.5) and presents a half-life of 2 h at 50 °C. Besides its activity towards pectin and polygalacturonic acid, the enzyme displays pectin-releasing activity, acting best ...
Source: Journal of Molecular Microbiology and Biotechnology - April 27, 2017 Category: Microbiology Source Type: research

Protection against Legionnaire's Disease: Recombinant Flagellin A of Legionella pneumophila Can Induce Protective Immunity against Bacteremia in a BALB/c Murine Model
To investigate the immunoprotective effects of the recombinant type A flagellin (FLA), theflaA gene ofLegionella pneumophila serogroup 1 strain Paris was cloned into pET28a(+). Recombinant FLA (rFLA) was overexpressed inE. coli BL21 (DE3) and purified by Ni2+ exchange chromatography. Female BALB/c aged 6-8 weeks were immunized with 20 μg of rFLA. Nonimmunized mice along with mice inoculated with a sublethal dose of liveL. pneumophila intravenously were considered as negative and positive controls, respectively. A significant serum antibody response was observed in female BALB/c mice immunized with rFLA. Production of IFN-...
Source: Journal of Molecular Microbiology and Biotechnology - April 25, 2017 Category: Microbiology Source Type: research

Protection against Legionnaire's Disease: Recombinant Flagellin A of Legionella pneumophila Can Induce Protective Immunity against Bacteremia in a BALB/c Murine Model
To investigate the immunoprotective effects of the recombinant type A flagellin (FLA), theflaA gene ofLegionella pneumophila serogroup 1 strain Paris was cloned into pET28a(+). Recombinant FLA (rFLA) was overexpressed inE. coli BL21 (DE3) and purified by Ni2+ exchange chromatography. Female BALB/c aged 6-8 weeks were immunized with 20 μg of rFLA. Nonimmunized mice along with mice inoculated with a sublethal dose of liveL. pneumophila intravenously were considered as negative and positive controls, respectively. A significant serum antibody response was observed in female BALB/c mice immunized with rFLA. Production of IFN-...
Source: Journal of Molecular Microbiology and Biotechnology - April 25, 2017 Category: Microbiology Source Type: research

Relative Strengths and Regulation of Different Promoter-Associated Sequences for Various blaSHV Genes and Their Relationships to β-Lactam Resistance
Conclusion: The mRNA expression and β-lactam resistance of theblaSHV showed consistencies and inconsistencies with the strengths of the promoter-associated sequences. The mechanisms accounting for these discrepancies need further investigation.J Mol Microbiol Biotechnol 2017;27:91-101 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - March 23, 2017 Category: Microbiology Source Type: research

Relative Strengths and Regulation of Different Promoter-Associated Sequences for Various bla SHV Genes and Their Relationships to β-Lactam Resistance
Conclusion: The mRNA expression and β-lactam resistance of theblaSHV showed consistencies and inconsistencies with the strengths of the promoter-associated sequences. The mechanisms accounting for these discrepancies need further investigation.J Mol Microbiol Biotechnol 2017;27:91-101 (Source: Journal of Molecular Microbiology and Biotechnology)
Source: Journal of Molecular Microbiology and Biotechnology - March 23, 2017 Category: Microbiology Source Type: research

Mating of 2 Laboratory Saccharomyces cerevisiae Strains Resulted in Enhanced Production of 2-Phenylethanol by Biotransformation of < smlcap > L < /smlcap > -Phenylalanine
2-Phenylethanol (2-PE) is an aromatic alcohol with a rosy scent which is widely used in the food, fragrance, and cosmetic industries. Promising sources of natural 2-PE are microorganisms, especially yeasts, which can produce 2-PE by biosynthesis and biotransformation. Thus, the first challenging goal in the development of biotechnological production of 2-PE is searching for highly productive yeast strains. In the present work, 5 laboratorySaccharomyces cerevisiae strains were tested for the production of 2-PE. Thereafter, 2 of them were hybridized by a mating procedure and, as a result, a new diploid,S. cerevisiae AM1-d, w...
Source: Journal of Molecular Microbiology and Biotechnology - February 24, 2017 Category: Microbiology Source Type: research

Mating of 2 Laboratory Saccharomyces cerevisiae Strains Resulted in Enhanced Production of 2-Phenylethanol by Biotransformation of < smlcap > L < /smlcap > -Phenylalanine
2-Phenylethanol (2-PE) is an aromatic alcohol with a rosy scent which is widely used in the food, fragrance, and cosmetic industries. Promising sources of natural 2-PE are microorganisms, especially yeasts, which can produce 2-PE by biosynthesis and biotransformation. Thus, the first challenging goal in the development of biotechnological production of 2-PE is searching for highly productive yeast strains. In the present work, 5 laboratorySaccharomyces cerevisiae strains were tested for the production of 2-PE. Thereafter, 2 of them were hybridized by a mating procedure and, as a result, a new diploid,S. cerevisiae AM1-d, w...
Source: Journal of Molecular Microbiology and Biotechnology - February 23, 2017 Category: Microbiology Source Type: research

Photometric Determination of Ammonium and Phosphate in Seawater Medium Using a Microplate Reader
To more efficiently process the large sample numbers for quantitative determination of ammonium (NH4+) and phosphate (orthophosphate, PO43-) generated during comprehensive growth experiments with the marineRoseobacter group member Phaeobacter inhibens DSM 17395, specific colorimetric assays employing a microplate reader (MPR) were established. The NH4+ assay is based on the reaction of NH4+ with hypochlorite and salicylate, yielding a limit of detection of 14 µM, a limit of quantitation of 36 µM, and a linear range for quantitative determination up to 200 µM. The PO43-assay is based on the complex formation of PO43- wit...
Source: Journal of Molecular Microbiology and Biotechnology - February 7, 2017 Category: Microbiology Source Type: research

Influences of Various Peptide Linkers on the Thermotoga maritima MSB8 Nitrilase Displayed on the Spore Surface of Bacillus subtilis
In the present study, fusion genes composed ofThermotoga maritima MSB8 nitrilase andBacillus subtilis 168 outer coat protein CotG were constructed with various peptide linkers and displayed onB. subtilis DB 403 spores. The successful display of CotG-nit fusion proteins on the spore surface ofB. subtilis was verified by Western blot analysis and activity measurement. It was demonstrated that the fusion with linker GGGGSEAAAKGGGGS presented the highest thermal and pH stability, which is 2.67- and 1.9-fold of the fusion without linker. In addition, fusion with flexible linker (GGGGS)3 demonstrated better thermal and pH stabil...
Source: Journal of Molecular Microbiology and Biotechnology - January 20, 2017 Category: Microbiology Source Type: research

Requirements for Septal Localization and Chromosome Segregation Activity of the DNA Translocase SftA from Bacillus subtilis 
Bacillus subtilis possesses 2 DNA translocases that affect late stages of chromosome segregation: SftA separates nonsegregated DNA prior to septum closure, while SpoIIIE rescues septum-entrapped DNA. We provide evidence that SftA is associated with the division machinery via a stretch of 47 amino acids within its N-terminus, suggesting that SftA is recruited by protein-protein interactions with a component of the division machinery. SftA was also recruited to mid-cell in the absence of its first 20 amino acids, which are proposed to contain a membrane-binding motif. Cell fractionation experiments showed that SftA can be fo...
Source: Journal of Molecular Microbiology and Biotechnology - January 20, 2017 Category: Microbiology Source Type: research