International Journal for Parasitology: Drugs and Drug Resistance This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Corrigendum to “Genetic diversity of next generation antimalarial targets: A baseline for drug resistance surveillance programmes” [Int. J. Parasitol. Drugs and Drug Resist. 7 (2017) 174–180]
Publication date: Available online 6 November 2017 Source:International Journal for Parasitology: Drugs and Drug Resistance Author(s): Ana Rita Gomes, Matt Ravenhall, Ernest Diez Benavente, Arthur Talman, Colin Sutherland, Cally Roper, Taane G. Clark, Susana Campino (Source: International Journal for Parasitology: Drugs and Drug Resistance)
Source: International Journal for Parasitology: Drugs and Drug Resistance - November 19, 2017 Category: Parasitology Source Type: research

Pathway of oxfendazole from the host into the worm: Trichuris suis in pigs
In this study, the pathway of oxfendazole and its metabolites was explored using a T. suis-pig infection model, by simultaneously measuring drug concentrations within the worms and the caecal mucosa, caecal tissue, caecal content and plasma of pigs over time after a single oral dose of 5 mg/kg oxfendazole. Additionally, for comparison to the in vivo study, drug uptake and metabolism of oxfendazole by T. suis was examined after in vitro incubation. Oxfendazole and metabolites were quantified by High Performance Liquid Chromatography. Multivariate linear regression analysis showed a strong and highly significant association...
Source: International Journal for Parasitology: Drugs and Drug Resistance - November 19, 2017 Category: Parasitology Source Type: research

Risk factor analysis of equine strongyle resistance to anthelmintics
This study brought together results from anthelmintic efficacy testing and risk factor analysis to provide evidence-based guidelines in the field. It involved 688 horses from 39 French horse farms and riding schools to both estimate Faecal Egg Count Reduction (FECR) after anthelmintic treatment and to interview farm and riding school managers about their practices. Risk factors associated with reduced anthelmintic efficacy in equine strongyles were estimated across drugs using a marginal modelling approach. Results demonstrated ivermectin efficacy (96.3% ± 14.5% FECR), the inefficacy of fenbendazole (42.8% ± 33.4% FE...
Source: International Journal for Parasitology: Drugs and Drug Resistance - November 19, 2017 Category: Parasitology Source Type: research

Antimalarial activity of Malaria Box Compounds against Plasmodium falciparum clinical isolates
This study investigated the potency of twenty compounds from the Malaria Box against four clinical isolates from Ghana, using optimized in vitro growth inhibitory assays. Seven out of the 20 compounds screened had 50% inhibitory concentration (IC50) below 500 nM. The most active among the selected compounds was MMV006087 (average IC50 of 30.79 nM). Variations in the potency of the Malaria Box Compounds were observed between P. falciparum clinical isolates and Dd2 strain. We also investigated the sensitivity of the clinical isolates to chloroquine and artesunate. The N093 clinical isolate was found to be resistant to chlo...
Source: International Journal for Parasitology: Drugs and Drug Resistance - November 19, 2017 Category: Parasitology Source Type: research

P-glycoproteins play a role in ivermectin resistance in cyathostomins
Publication date: December 2017 Source:International Journal for Parasitology: Drugs and Drug Resistance, Volume 7, Issue 3 Author(s): L.E. Peachey, G.L. Pinchbeck, J.B. Matthews, F.A. Burden, A. Lespine, G. von Samson-Himmelstjerna, J. Krücken, J.E. Hodgkinson Anthelmintic resistance is a global problem that threatens sustainable control of the equine gastrointestinal cyathostomins (Phylum Nematoda; Superfamily Strongyloidea). Of the three novel anthelmintic classes that have reached the veterinary market in the last decade, none are currently licenced in horses, hence current control regimens focus on prolongi...
Source: International Journal for Parasitology: Drugs and Drug Resistance - November 19, 2017 Category: Parasitology Source Type: research

Transcriptome profiling identifies genes/pathways associated with experimental resistance to paromomycin in Leishmania donovani
In this study, PMM resistant lines were selected up to 100 μM of PMM in three distinct field isolates of Leishmania donovani at promastigote stage. The resistance induced at promastigote level was also evident in amastigotes which showed 6 fold decreases in PMM susceptibility. Comparative transcriptome profiling of PMM resistant (PMM-R) and the corresponding PMM sensitive (PMM-S) parasites revealed modulated expression of 500 genes (1.5 fold cut off) in PMM-R parasites. Selected genes were validated for their modulated expression by quantitative real-time PCR. Functional classification and pathway analysis of modulated g...
Source: International Journal for Parasitology: Drugs and Drug Resistance - October 11, 2017 Category: Parasitology Source Type: research

Comparison of constitutive and thiabendazole-induced expression of five cytochrome P450 genes in fourth-stage larvae of Haemonchus contortus isolates with different drug susceptibility identifies one gene with high constitutive expression in a multi-resistant isolate
Publication date: Available online 7 October 2017 Source:International Journal for Parasitology: Drugs and Drug Resistance Author(s): Esra Yilmaz, Sabrina Ramünke, Janina Demeler, Jürgen Krücken Benzimidazoles (BZs) remain amongst the most widely used anthelmintic drug classes against gastro-intestinal nematode infections, although their efficacy is increasingly compromised by resistance. The primary underlying mechanisms for BZ resistance are single-nucleotide polymorphisms (SNPs) in the isotype 1 β-tubulin gene causing the substitutions F167Y, E198A or F200Y. However, resistance is believed to be multi-genic an...
Source: International Journal for Parasitology: Drugs and Drug Resistance - October 8, 2017 Category: Parasitology Source Type: research

Treatment with Fenofibrate plus a low dose of Benznidazole attenuates cardiac dysfunction in experimental Chagas disease
In this study we investigated whether the PPAR-α agonist, Fenofibrate, improves cardiac function and inflammatory parameters in a murine model of T. cruzi infection. BALB/c mice were sequentially infected with two T. cruzi strains of different genetic background. Benznidazole, commonly used as trypanocidal drug, cleared parasites but did not preclude cardiac pathology, resembling what is found in human chronic chagasic cardiomyopathy. Fenofibrate treatment restored to normal values the ejection and shortening fractions, left ventricular end-diastolic, left ventricular end-systolic diameter, and isovolumic relaxation time....
Source: International Journal for Parasitology: Drugs and Drug Resistance - October 8, 2017 Category: Parasitology Source Type: research

Genomic analysis of Isometamidium Chloride resistance in Trypanosoma congolense
Publication date: Available online 6 October 2017 Source:International Journal for Parasitology: Drugs and Drug Resistance Author(s): Eliane Tihon, Hideo Imamura, Frederik Van den Broeck, Lieve Vermeiren, Dujardin Jean-Claude, Jan Van Den Abbeele Isometamidium Chloride (ISM) is one of the principal drugs used to counteract Trypanosoma congolense infection in livestock, both as a prophylactic as well as a curative treatment. However, numerous cases of ISM resistance have been reported in different African regions, representing a significant constraint in the battle against Animal African Trypanosomiasis. In order to...
Source: International Journal for Parasitology: Drugs and Drug Resistance - October 7, 2017 Category: Parasitology Source Type: research

L-Asparaginase of Leishmania donovani: Metabolic target and its role in Amphotericin B resistance
Publication date: Available online 28 September 2017 Source:International Journal for Parasitology: Drugs and Drug Resistance Author(s): Jasdeep Singh, Mohd Imran Khan, Shiv Pratap Singh Yadav, Ankit Srivastava, Kislay K. Sinha, Ashish, Pradeep Das, Bishwajit Kundu Emergence of Amphotericin B (AmB) resistant Leishmania donovani has posed major therapeutic challenge against the parasite. Consequently, combination therapy aimed at multiple molecular targets, based on proteome wise network analysis has been recommended. In this regard we had earlier identified and proposed L-asparaginase of Leishmania donovani (LdAI...
Source: International Journal for Parasitology: Drugs and Drug Resistance - September 28, 2017 Category: Parasitology Source Type: research

In  vitro evaluation of antimicrobial agents on Acanthamoeba sp. and evidence of a natural resilience to amphotericin B
Publication date: December 2017 Source:International Journal for Parasitology: Drugs and Drug Resistance, Volume 7, Issue 3 Author(s): Alexandre Taravaud, Philippe M. Loiseau, Sébastien Pomel The free-living amoeba (FLA) Acanthamoeba sp. is an opportunistic pathogen that can cause amoebic keratitis (AK) or granulomatous amoebic encephalitis (GAE). While current treatments of AK are long with some relapses, no consensus therapy has been developed for GAE remaining lethal in 90% of the cases. In this context, efficient antiacanthamoebal drugs have to be identified. In this work, 15 drugs used in the treatment of AK or ...
Source: International Journal for Parasitology: Drugs and Drug Resistance - September 15, 2017 Category: Parasitology Source Type: research

In vitro evaluation of antimicrobial agents on Acanthamoeba sp. and evidence of a natural resilience to amphotericin B
Publication date: Available online 8 September 2017 Source:International Journal for Parasitology: Drugs and Drug Resistance Author(s): Alexandre Taravaud, Philippe M. Loiseau, Sébastien Pomel The free-living amoeba (FLA) Acanthamoeba sp. is an opportunistic pathogen that can cause amoebic keratitis (AK) or granulomatous amoebic encephalitis (GAE). While current treatments of AK are long with some relapses, no consensus therapy has been developed for GAE remaining lethal in 90% of the cases. In this context, efficient antiacanthamoebal drugs have to be identified. In this work, 15 drugs used in the treatment of AK or...
Source: International Journal for Parasitology: Drugs and Drug Resistance - September 9, 2017 Category: Parasitology Source Type: research

Drug susceptibility testing in microaerophilic parasites: Cysteine strongly affects the effectivities of metronidazole and auranofin, a novel and promising antimicrobial
In this study, it was attempted to reduce cysteine concentrations as far as possible without affecting parasite viability by performing drug susceptibility assays under strictly anaerobic conditions in an anaerobic cabinet. Indeed, T. vaginalis and E. histolytica could be grown without any cysteine added and the cysteine concentration necessary to maintain G. lamblia could be reduced to 20%. Susceptibilities to metronidazole were found to be clearly reduced in the presence of cysteine. With auranofin the protective effect of cysteine was extreme, providing protection to concentrations up to 100-fold higher as observed in t...
Source: International Journal for Parasitology: Drugs and Drug Resistance - September 5, 2017 Category: Parasitology Source Type: research

Diglycosyl diselenides alter redox homeostasis and glucose consumption of infective African trypanosomes
Publication date: Available online 12 August 2017 Source:International Journal for Parasitology: Drugs and Drug Resistance Author(s): Jaime Franco, Florencia Sardi, László Szilágyi, Katalin E. Kövér, Krisztina Fehér, Marcelo A. Comini With the aim to develop compounds able to target multiple metabolic pathways and, thus, to lower the chances of drug resistance, we investigated the anti-trypanosomal activity and selectivity of a series of symmetric diglycosyl diselenides and disulfides. Of 18 compounds tested the fully acetylated forms of di-β-D-glucopyranosyl and di-β-D-galactopyranosyl diselenides (13 and ...
Source: International Journal for Parasitology: Drugs and Drug Resistance - August 12, 2017 Category: Parasitology Source Type: research

Antiplasmodial activities of dyes against Plasmodium falciparum asexual and sexual stages: Contrasted uptakes of triarylmethanes Brilliant green, Green S (E142), and Patent Blue V (E131) by erythrocytes
Publication date: Available online 2 August 2017 Source:International Journal for Parasitology: Drugs and Drug Resistance Author(s): Louis-Jérôme Leba, Jean Popovici, Yannick Estevez, Stéphane Pelleau, Eric Legrand, Lise Musset, Christophe Duplais The search for safe antimalarial compounds acting against asexual symptom-responsible stages and sexual transmission-responsible forms of Plasmodium species is one of the major challenges in malaria elimination programs. So far, among current drugs approved for human use, only primaquine has transmission-blocking activity. The discovery of small molecules targeting di...
Source: International Journal for Parasitology: Drugs and Drug Resistance - August 2, 2017 Category: Parasitology Source Type: research