Dietary arachidonic acid decreases the expression of transcripts related to adipocyte development and chronic inflammation in the adipose tissue of juvenile grass carp, Ctenopharyngodon idella
Publication date: Available online 22 February 2019Source: Comparative Biochemistry and Physiology Part D: Genomics and ProteomicsAuthor(s): Jing-jing Tian, Cai-xia Lei, Hong Ji, Ji-shu Zhou, Hai-bo Yu, Yang Li, Er-meng Yu, Jun XieAbstractOverdevelopment of adipose tissue in cultured fish is one of the biggest issues plaguing current aquaculture industry, leading to unhealthy status of fishes and production losses. Diet supplemented with 0.30% arachidonic acid (ARA) has been found to reduce adipogenesis and inflammation in grass carp, but the potential mechanism is not comprehensively understood. To fully reveal the effect...
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - February 23, 2019 Category: Genetics & Stem Cells Source Type: research

Editorial Board
Publication date: March 2019Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, Volume 29Author(s): (Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics)
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - February 20, 2019 Category: Genetics & Stem Cells Source Type: research

Midgut de novo transcriptome analysis and gene expression profiling of Achaea janata larvae exposed with Bacillus thuringiensis (Bt)-based biopesticide formulation
Publication date: Available online 15 February 2019Source: Comparative Biochemistry and Physiology Part D: Genomics and ProteomicsAuthor(s): Narender K. Dhania, Vinod K. Chauhan, R.K. Chaitanya, Aparna Dutta-GuptaAbstractIndia is the major producer and exporter of castor oil in the world. Castor semilooper, Achaea janata is one of the main castor crop pests, which causes serious economic loss of crop, hence management and control of the pest are important. Currently, Bacillus thuringiensis (Bt) based biopesticides are being used for their control. However, the insects are known to develop resistance not only against chemic...
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - February 16, 2019 Category: Genetics & Stem Cells Source Type: research

The expression of genes involved in excitatory and inhibitory neurotransmission in turtle (Trachemys scripta) brain during anoxic submergence at 21 °C and 5 °C reveals the importance of cold as a preparatory cue for anoxia survival
Publication date: Available online 13 February 2019Source: Comparative Biochemistry and Physiology Part D: Genomics and ProteomicsAuthor(s): Christine S. Couturier, Jonathan A.W. Stecyk, Stian Ellefsen, Guro K. Sandvik, Sarah L. Milton, Howard M. Prentice, Göran E. NilssonAbstractWe investigated if transcriptional responses are consistent with the arrest of synaptic activity in the anoxic turtle (Trachemys scripta) brain. Thirty-nine genes of key receptors, transporters, enzymes and regulatory proteins of inhibitory and excitatory neurotransmission were partially cloned and their expression in telencephalon of 21 °C- a...
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - February 13, 2019 Category: Genetics & Stem Cells Source Type: research

Identification and expression profiling of candidate chemosensory membrane proteins in the band-winged grasshopper, Oedaleus asiaticus
In this study, a total of 69 candidate chemosensory membrane protein genes, including 60 olfactory receptors (ORs), 6 ionotropic receptors (IRs) and 3 sensory neuron membrane proteins (SNMPs), were identified for the first time from the antennal transcriptomes of O. asiaticus. Blastp match and phylogenetic analysis demonstrated that these chemosensory membrane proteins were the closest to their orthologous species, Locusta migratoria. The qRT-PCR analysis revealed that all tested 14 OR and two SNMP genes (OasiSNMP1 and OasiSNMP2a) were strongly expressed in adult antennae, and nearly all tested genes (15/16) displayed sign...
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - February 11, 2019 Category: Genetics & Stem Cells Source Type: research

Genome-wide analysis of developmental stage-specific transcriptome in Bradysia odoriphaga
This study identified DEUs for sequential developmental stages of B. odoriphaga. Gene Ontology classifications and KEGG pathway identification of DEUs not only provide information useful for understanding insect growth and development but also for exploring novel approaches to control B. odoriphaga. (Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics)
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - February 10, 2019 Category: Genetics & Stem Cells Source Type: research

1H NMR metabolomic analysis of skin and blubber of bottlenose dolphins reveal a functional metabolic dichotomy
Publication date: Available online 10 February 2019Source: Comparative Biochemistry and Physiology Part D: Genomics and ProteomicsAuthor(s): Biswapriya Biswavas Misra, Ruiz-Hernández Ixchel Mariel, Hernández-Bolio Gloria Ivonne, Hernández-Núñez Emanuel, Díaz-Gamboa Raúl, Colli-Dula Reyna CristinaAbstractThe common bottlenose dolphin (Tursiops truncatus) is a carnivorous cetacean thriving in marine environment that is one of the apex predators of the marine food web. They are found in coastal and estuarine ecosystems which are known to be sensitive to environmental impacts. Dolphin is considered sentinel organisms fo...
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - February 10, 2019 Category: Genetics & Stem Cells Source Type: research

Hemolymph and transcriptome analysis to understand innate immune responses to hypoxia in Pacific abalone
Publication date: Available online 6 February 2019Source: Comparative Biochemistry and Physiology Part D: Genomics and ProteomicsAuthor(s): Yawei Shen, Zekun Huang, Guangmou Liu, Caihuan Ke, Weiwei YouAbstractHypoxia was thought to inhibit immune responses, causing severe mortality to marine organisms. The Pacific abalone, Haliotis discus hannai, is the most widely cultured abalone species in China, but suffering from “summer mortality” in which hypoxia has been one of the main reasons. The effect of hypoxia exposure on immune responses in H. discus hannai was investigated, including cellular immune response using flow...
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - February 6, 2019 Category: Genetics & Stem Cells Source Type: research

Comparative analysis of the high molecular mass subproteomes of eight Bothrops snake venoms
This study sought to achieve detailed identification of major components in the high molecular mass subproteome of venoms from eight Bothrops species (B. brazili, B. cotiara, B. insularis, B. jararaca, B. jararacussu, B. leucurus, B. moojeni and B. neuwiedi). Enzymes such as metalloproteinases and L-amino acid oxidases were the most prominent components identified in the first size-exclusion chromatography fractions of these venoms. Minor components also identified in the first peaks included 5′-nucleotidase, aminopeptidase, phosphodiesterase, and phospholipases A2 and B. Most of these components disappeared in electroph...
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - January 31, 2019 Category: Genetics & Stem Cells Source Type: research

mRNA and microRNA transcriptomics analyses in intermuscular bones of two carp species, rice flower carp (Cyprinus carpio var. Quanzhounensis) and Jian carp (Cyprinus carpio var. Jian)
In this study, we analyzed the mRNA and microRNA (miRNA) transcriptomes in the intermuscular bones of rice flower carp and Jian carp (Cyprinus carpio var. Jian) (a typical member of common carp in China), using Illumina RNA sequencing. We identified 55,340 genes (including 47,541 known genes and 8231 predicted new genes) and 662 miRNAs (including 595 known miRNAs and 67 novel miRNAs) in the two species. By comparing the transcriptomes of the two species, we identified 1523 differentially expressed genes (DEGs) (including 576 up - and 947 downregulated DEGs) and 352 differentially expressed miRNAs (DEMs) (including 85 up- a...
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - January 30, 2019 Category: Genetics & Stem Cells Source Type: research

Pacific geoduck (Panopea generosa) resilience to natural pH variation
In this study, eelgrass habitats and environmental heterogeneity across four estuarine bays were leveraged to examine low pH effects on geoduck under different natural regimes, using targeted proteomics to assess physiology. Juvenile geoduck were deployed in eelgrass and adjacent unvegetated habitats for 30 days while pH, temperature, dissolved oxygen, and salinity were monitored. Across the four bays, pH was lower in unvegetated habitats compared to eelgrass habitats. However this did not impact geoduck growth, survival, or proteomic abundance patterns in gill tissue. Temperature and dissolved oxygen differences across ...
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - January 25, 2019 Category: Genetics & Stem Cells Source Type: research

Glucose and urea metabolic enzymes are differentially phosphorylated during freezing, anoxia, and dehydration exposures in a freeze tolerant frog
Publication date: Available online 25 January 2019Source: Comparative Biochemistry and Physiology Part D: Genomics and ProteomicsAuthor(s): Liam J. Hawkins, Minjing Wang, Baowen Zhang, Qi Xiao, Hui Wang, Kenneth B. StoreyAbstractVertebrate freeze tolerance requires multiple adaptations underpinned by specialized biochemistry. Freezing of extracellular water leads to intracellular dehydration as pure water is incorporated into growing ice crystals and also results in the cessation of blood supply to tissues, creating an anoxic cellular environment. Hence, the freeze tolerant wood frog, Rana sylvatica, must endure both dehyd...
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - January 25, 2019 Category: Genetics & Stem Cells Source Type: research

Metabolic responses to elevated pCO2 in the gills of the Pacific oyster (Crassostrea gigas) using a GC-TOF-MS-based metabolomics approach
Publication date: Available online 18 January 2019Source: Comparative Biochemistry and Physiology Part D: Genomics and ProteomicsAuthor(s): Zengjie Jiang, Xiaoqin Wang, Samuel P.S. Rastrick, Jinghui Fang, Meirong Du, Yaping Gao, Fengxue Li, Øivind Strand, Jianguang FangAbstractRising atmospheric carbon dioxide (CO2), primarily from anthropogenic emissions, are resulting in increasing absorption of CO2 by the oceans, leading to a decline in oceanic pH in a process known as ocean acidification (OA). There is a growing body of evidence demonstrating the potential effect of OA on the energetics/physiology and consequently lif...
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - January 18, 2019 Category: Genetics & Stem Cells Source Type: research

Niemann-Pick proteins type C2 are identified as olfactory related genes of Pardosa pseudoannulata by transcriptome and expression profile analysis
In this study, six NPC2s namely PpseNPC1-6 were newly identified in the appendages of P. pseudoannulata based on transcriptome data. A phylogenetic analysis indicated that all of P. pseudoannulata NPC2s were clustered together forming one clade with high posterior probability values. In addition, the sequences shared the same subclade with the NPC2 sequences of ticks and scorpion. The motif-patterns indicated that PpseNPC2-5 had the common pattern with the two-spotted spider mite Tetranychus urticae and the ant Trachymyrmex cornetzi. Furthermore, quantitative real-time PCR (qPCR) measurements were conducted to evaluate the...
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - January 17, 2019 Category: Genetics & Stem Cells Source Type: research

Global metabolic responses of the lenok (Brachymystax lenok) to thermal stress
In this study, a NMR-based metabonomic strategy in combination with high-throughput RNA-Seq was employed to investigate global metabolic changes of plasma and liver in a typical cold water fish species lenok (Brachymystax lenok) subjected to a sub-lethal high temperature. Our results showed that thermal stress caused multiple dynamic metabolic alterations of the lenok with prolonged stress, including repression of energy metabolism, shifts in lipid metabolism, alterations in amino acid metabolism, changes in choline and nucleotide metabolisms. Specifically, thermal stress induced an activation of glutamate metabolism, indi...
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - January 16, 2019 Category: Genetics & Stem Cells Source Type: research