Effect of temperature on the microflora community composition in the digestive tract of the veined rapa whelk (Rapana venosa) revealed by 16S rRNA gene sequencing
Publication date: Available online 23 October 2018Source: Comparative Biochemistry and Physiology Part D: Genomics and ProteomicsAuthor(s): Mei-Jie Yang, Hao Song, Li-Na Sun, Zheng-Lin Yu, Zhi Hu, Xiao-Long Wang, Jia-Yi Zhu, Tao ZhangAbstractThe veined rapa whelk, Rapana venosa, a poikilotherm that is susceptible to temperature, is an important and valuable fishery resource in China but a major invader around the world. We studied the effects of abnormal temperature on the digestive tract microflora of R. venosa to investigate how temperature impacts its digestion and ingestion. We characterized the microflora in nine samp...
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - October 24, 2018 Category: Genetics & Stem Cells Source Type: research

Integration of small RNAs and mRNAs by high-throughput sequencing reveals a complex regulatory network in Chinese sea cucumber, Russian sea cucumber and their hybrids
In this study, the parents of Apostichopus japonicus were derived from Russia (R) and China (C). We examined the transcriptional profiles in the body wall from F1 hybrids (CR and RC) and purebreds (CC and RR) at 9 months. In total, 34.07 G clean data were obtained from the transcriptome, 100,870 unigenes were identified, and 26,483 unigenes were annotated. Based on edgeR, 1097–4,587differentially expressed genes (DEGs) were identified. In total, 50.72 M clean data were obtained from the small RNA libraries, and 202 miRNAs were identified, including 7 known miRNAs and 195 novel miRNAs. In total, 175 miRNAs were iden...
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - October 21, 2018 Category: Genetics & Stem Cells Source Type: research

Transcriptional shifts during juvenile Coho salmon (Oncorhynchus kisutch) life stage changes in freshwater and early marine environments
Publication date: Available online 18 October 2018Source: Comparative Biochemistry and Physiology Part D: Genomics and ProteomicsAuthor(s): Aimee Lee S. Houde, Angela D. Schulze, Karia H. Kaukinen, Jeffrey Strohm, David A. Patterson, Terry D. Beacham, Anthony P. Farrell, Scott G. Hinch, Kristina M. MillerAbstractThere is a paucity of information on the physiological changes that occur over the course of salmon early marine migration. Here we aim to provide insight on juvenile Coho salmon (Oncorhynchus kisutch) physiology using the changes in gene expression (cGRASP 44K microarray) of four tissues (brain, gill, muscle, and ...
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - October 19, 2018 Category: Genetics & Stem Cells Source Type: research

Identification and characterization of microRNAs involved in scale biomineralization in the naked carp Gymnocypris przewalskii
Publication date: Available online 9 October 2018Source: Comparative Biochemistry and Physiology Part D: Genomics and ProteomicsAuthor(s): Shiguo Li, Weiwei Chen, Aibin Zhan, Jian LiangAbstractThe mineralized scale derived from skin plays a protective role for the fish body and also possesses important application values in the biomaterial field. However, little is known about fish scale biomineralization and related molecular regulatory mechanisms. Here, we used a comparative microRNA sequencing approach to identify and characterize differentially expressed microRNAs (DEMs) involved in scale biomineralization in the naked...
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - October 9, 2018 Category: Genetics & Stem Cells Source Type: research

Transcriptome from Pacific cod liver reveals types of apolipoproteins and expression analysis of AFP-IV, structural analogue with mammalian ApoA-I
In this study, ApoA-I, ApoA-I-2, ApoA-IV, Apo E, ApoB-100-like and AFP-IV were sequenced from Pacific cod (Gadus macrocephalus) liver transcriptome using Illumina HiSeq 2000, and their 3-D models were constructed based on the most confidence templates ever reported in mammals. Interestingly, the model of G. macrocephalus AFP-IV, named GmAFPIV, is quite similar to the structure of ApoA-I. GmAFPIV includes 689 bases with a complete open reading frame encoding 125 amino acids. Sequence alignment of GmAFPIV showed 30% to 50% similarity with that of other species except Gadus sp. Expression levels of GmAFPIV were found in a dec...
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - October 9, 2018 Category: Genetics & Stem Cells Source Type: research