Sub-lethal effects of calcium dinonylnaphthalenesulfonate on Western clawed frog embryos
Publication date: Available online 29 January 2020Source: Comparative Biochemistry and Physiology Part D: Genomics and ProteomicsAuthor(s): S.J. Wallace, A.J.A. Leclerc, R. Prosser, S.R. de Solla, V. Balakrishnan, V.S. LangloisAbstractNaphthalene sulfonic acids (NSAs) are used as additives in lubricants, dyes, and greases and commonly act as surfactants in many industrial processes. The calcium salt of dinonyl NSA (calcium dinonylnaphthalenesulfonate; CaDNS) is listed among thousands of chemicals identified as priorities for assessment by the Government of Canada's Chemical Management Plan due to the limited toxicity data....
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - January 30, 2020 Category: Genetics & Stem Cells Source Type: research

Antennal transcriptome analysis and expression profiles of putative chemosensory soluble proteins in Histia rhodope Cramer (Lepidoptera: Zygaenidae)
In this study, we established antennal transcriptome of H. rhodope and ultimately identified 19 odorant binding proteins (OBPs), 23 chemosensory proteins (CSPs) and 4 Niemann-Pick type C2 proteins (NPC2s). The 19 OBPs, 6 CSPs and 4 NPC2s were assessed to validate the differential expressions between sexes, and between olfactory and non-olfactory tissues. 8 OBPs and 2 CSPs exhibited male-biased antennae expression, while 6 OBPs, 2 CSPs and HrhoNPC2a exhibited female-biased antennae expression. Moreover, 17 OBPs, 4 CSPs and 2 NPC2s were predominantly expressed in the antennae compared with non-olfactory tissues. HrhoOBP1 and...
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - January 12, 2020 Category: Genetics & Stem Cells Source Type: research

Effect of glycogen synthase and glycogen phosphorylase knockdown on the expression of glycogen- and insulin-related genes in the rice brown planthopper Nilaparvata lugens
Publication date: Available online 3 January 2020Source: Comparative Biochemistry and Physiology Part D: Genomics and ProteomicsAuthor(s): Bo-Ping Zeng, Kui Kang, Hui-Juan Wang, Bi-Ying Pan, Cai-Di Xu, Bin Tang, Dao-Wei ZhangAbstractNilaparvata lugens is a serious threat to rice growth. Glycogen metabolism is one of the important physiological processes of insects, which is mainly regulated by glycogen synthase (GS) and glycogen phosphorylase (GP). In the present study, trehalose content was significantly reduced at 72 h after NlGP and NlGS knockdown, whereas glucose content was significantly increased at both 48 h and...
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - January 5, 2020 Category: Genetics & Stem Cells Source Type: research

Structure and function analysis of various brain subregions and pituitary in grass carp (Ctenopharyngodon idellus)
This study provided a global view of the microstructures and specific functions of various brain subregions and pituitary in teleost. These results will be very helpful for further study in the neuroendocrinology regulation of growth and reproduction in teleost brain-pituitary axis.Graphical abstract (Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics)
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - January 5, 2020 Category: Genetics & Stem Cells Source Type: research

Transcriptome changes for Nile tilapia (Oreochromis niloticus) in response to alkalinity stress
In this study, we employed high throughput RNA sequencing to reveal the tissues (gill, kidney and liver) transcriptome differences of O. niloticus at different carbonate alkalinities (FW, AW40 and AW60). A total of 1,369,381,790 raw reads were obtained, including 496,441,232 reads in FW group, 437,907,696 reads in AW40 and 435,032,862 reads in AW60. In addition, 484,555,626 reads in gill, 451,618,224 reads in kidney and 433,207,940 reads in liver. A large number of stress-regulated changes were detected comprehensively. We focused on 3 significantly change pathways (steroid biosynthesis, drug metabolism and protein digesti...
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - January 3, 2020 Category: Genetics & Stem Cells Source Type: research

The distinct transcriptomes of fast-twitch and slow-twitch muscles in Mongolian horses
We examined the muscle fiber type population and produced deep RNA sequencing for different parts of skeletal muscles. And chose two of them with the highest difference in fast and slow muscle fiber population (splenius and gluteus medius) for comparing the gene expression profile of slow and fast muscle fiber types. We identified a total of 275 differentially expressed genes (DEGs), and 11 differentially expressed miRNAs (DEmiRs). In addition, target gene prediction and alternative splicing analysis were also performed. Significant correlations were found between the differentially expressed gene, miRNAs, and alternative ...
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - December 11, 2019 Category: Genetics & Stem Cells Source Type: research

SEM observation of early shell formation and expression of biomineralization-related genes during larval development in the pearl oyster Pinctada fucata
Publication date: Available online 2 December 2019Source: Comparative Biochemistry and Physiology Part D: Genomics and ProteomicsAuthor(s): Haimei Li, Lirong Bai, Xiaoyun Dong, Xiaohui Qi, Hongying Liu, Dahui YuAbstractShell formation of Pinctada fucata in larval development stages plays a crucial role in their survival. Scanning electron microscopy (SEM) was used to observe the morphological changes during larval development. We found that the early shell forms soon after enlargement of the blastopore at the anterior end of the trochophore stage and the complete shell forms in the spats stage, required for metamorphosis o...
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - December 3, 2019 Category: Genetics & Stem Cells Source Type: research

Identification and comparative expression profiles of chemosensory genes in major chemoreception organs of a notorious pests, Laodelphax striatellus
In this study, we identified multi-gene chemosensory families from the transcriptome of SBPH olfactory organs and analyzed their expression patterns in male and female tissues. Among the chemosensory genes, 14 odorant-binding proteins (OBPs), 12 chemosensory proteins (CSPs), 7 sensory neuron membrane proteins (SNMPs) and 95 odorant receptors (ORs) were identified and annotated in SBPH olfactory organs. Based on expression profile and phylogenetic analysis, LstrOBP1, 2, 5, 6, 7, 10, LstrSNMP1, and most LstrORs showed an antennae-enriched expression pattern, which suggests an olfactory role for these genes. Relative expressi...
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - December 3, 2019 Category: Genetics & Stem Cells Source Type: research

Genome-wide identification of JNK and p38 gene family in Ctenopharyngodon idella and their expression profiles in response to bacterial challenge
In this study, we analyzed the whole-genome database of the grass carp and identified 4 JNK and 6 p38 genes. JNK and p38 genes of grass carp were distributed in 7 out of 24 chromosomes. All JNK and p38 proteins contained characteristic dual-phosphorylation site. The JNKs contain a specific dual-phosphorylation consensus ((Thr-Pro-Tyr) that is different from that of the p38 proteins (Thr-Gly-Tyr). Deduced gene secondary structure analyses as well as the syntenic analyses further supported their annotation and orthologs. Results of tissue distribution detection revealed that JNK and p38 genes exhibited lower expression in he...
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - November 29, 2019 Category: Genetics & Stem Cells Source Type: research

Integrative microRNA and mRNA analysis reveals regulation of ER stress in the Pacific white shrimp Litopenaeus vannamei under acute cold stress
Publication date: Available online 29 November 2019Source: Comparative Biochemistry and Physiology Part D: Genomics and ProteomicsAuthor(s): Zhenlu Wang, Yongyong Feng, Junyi Li, Jixing Zou, Lanfen FanAbstractLitopenaeus vannamei (L. vannamei) is one of the most important aquaculture shrimps in the world and its survival, growth, and distribution was seriously challenged by cold stress. To investigate the response of L. vannamei under acute cold stress, mRNA and microRNA (miRNA) profiles of the hepatopancreas which was collected from the control group (28 °C) and the treatment group (13 °C) were analyzed. The results...
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - November 29, 2019 Category: Genetics & Stem Cells Source Type: research

Blubber proteome response to repeated ACTH administration in a wild marine mammal
Publication date: Available online 22 November 2019Source: Comparative Biochemistry and Physiology Part D: Genomics and ProteomicsAuthor(s): Jared Deyarmin, Ryan Hekman, Cory Champagne, Molly McCormley, Alicia Stephan, Daniel Crocker, Dorian Houser, Jane KhudyakovAbstractWhile the response to acute stress is adaptive in nature, repeated or chronic stress can impact an animal's fitness by depleting its energy stores and suppressing immune function and reproduction. This can be especially deleterious for species that rely on energy reserves to fuel key life history stages (e.g. reproduction), already experience physiological...
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - November 22, 2019 Category: Genetics & Stem Cells Source Type: research

Editorial Board
Publication date: December 2019Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, Volume 32Author(s): (Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics)
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - November 13, 2019 Category: Genetics & Stem Cells Source Type: research

Chronic exposure to venlafaxine and increased water temperature reversibly alters microRNA in zebrafish gonads (Danio rerio)
Publication date: Available online 4 November 2019Source: Comparative Biochemistry and Physiology Part D: Genomics and ProteomicsAuthor(s): Heather Ikert, Paul M. CraigAbstractMicroRNA (miRNA) are short, non-coding RNA that act by downregulating targeted mRNA transcripts. Only recently have they been used as endpoints in studies of aquatic toxicology. The purpose of this study was to determine the effect of an antidepressant contaminant, venlafaxine (VFX), and increased temperature on specific microRNA levels in zebrafish (Danio rerio) reproductive tissue. Adult zebrafish were exposed to one of four conditions; control, 1â...
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - November 6, 2019 Category: Genetics & Stem Cells Source Type: research

Transcriptome analysis of golden pompano (Trachinotus ovatus) liver indicates a potential regulatory target involved in HUFA uptake and deposition
This study focused on the liver, which is the center of intermediary metabolism, providing a comprehensive understanding of the molecular regulation of HUFA uptake and deposition in T. ovatus, which should be further investigated to develop potential measures to improve HUFA content. (Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics)
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - November 6, 2019 Category: Genetics & Stem Cells Source Type: research

Transcriptome analysis and weighted gene co-expression network reveals potential genes responses to heat stress in turbot Scophthalmus maximus
In this study, we performed transcriptome gene expression profiling of kidney tissue in turbot exposed to heat stress (20, 23, 25 and 28 °C); control fish were maintained at 14 °C. We investigated gene relationships based on weighted gene co-expression network analysis (WGCNA). Accordingly, enrichment analyses of GO terms and KEGG pathways showed that several pathways (e.g., fat metabolism, cell apoptosis, immune system, and insulin signaling) may be involved in the response of turbot to heat stress. Moreover, via WGCNA, we identified 19 modules: the dark grey module was mainly enriched in pathways associated with fa...
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - November 1, 2019 Category: Genetics & Stem Cells Source Type: research