Metabolic responses to elevated pCO2 in the gills of the Pacific oyster (Crassostrea gigas) using a GC-TOF-MS-based metabolomics approach

Publication date: Available online 18 January 2019Source: Comparative Biochemistry and Physiology Part D: Genomics and ProteomicsAuthor(s): Zengjie Jiang, Xiaoqin Wang, Samuel P.S. Rastrick, Jinghui Fang, Meirong Du, Yaping Gao, Fengxue Li, Øivind Strand, Jianguang FangAbstractRising atmospheric carbon dioxide (CO2), primarily from anthropogenic emissions, are resulting in increasing absorption of CO2 by the oceans, leading to a decline in oceanic pH in a process known as ocean acidification (OA). There is a growing body of evidence demonstrating the potential effect of OA on the energetics/physiology and consequently life-history traits of commensally important marine organisms. However, despite this little is known of how fundamental metabolic pathways that underpin changes in organismal physiology are affected by OA. Consequently, a gas chromatography time-of-flight mass spectrometry (GC-TOF-MS) based metabolic profiling approach was applied to examine the metabolic responses of Crassostrea gigas to elevated pCO2 levels, under otherwise natural field conditions. Oysters were exposed natural environmental pCO2 (~625.40 μatm) and elevated pCO2 (~1432.94 μatm) levels for 30 days. Results indicated that 36 differential metabolites were identified. Differential metabolites were mapped in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database to search for the related metabolic pathways. Pathway enrichment analysis indicates that alanine, aspartate and glutamate me...
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - Category: Genetics & Stem Cells Source Type: research