Dynamin's helical geometry does not destabilize membranes during fission
Traffic, EarlyView. (Source: Traffic)
Source: Traffic - March 25, 2018 Category: Research Source Type: research
Intersection of endocytic and exocytic systems in Toxoplasma gondii
Traffic, EarlyView. (Source: Traffic)
Source: Traffic - March 25, 2018 Category: Research Source Type: research
Sac1, a lipid phosphatase at the interface of vesicular and nonvesicular transport
Traffic, EarlyView. (Source: Traffic)
Source: Traffic - March 25, 2018 Category: Research Source Type: research
The biology of extracellular microvesicles
Traffic, EarlyView. (Source: Traffic)
Source: Traffic - March 25, 2018 Category: Research Source Type: research
Issue Information
Traffic,Volume 19, Issue 4, Page 243-245, April 2018. (Source: Traffic)
Source: Traffic - March 25, 2018 Category: Research Source Type: research
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Traffic, Ahead of Print. (Source: Traffic)
Source: Traffic - March 25, 2018 Category: Research Source Type: research
Rapid and dynamic arginylation of the leading edge β‐actin is required for cell migration
This study demonstrates that β‐actin arginylation occurs primarily at the very edge of migrating cells, that it responds rapidly to cell stimulation inducing migratory behavior, and that its inhibition severely impairs cell migration. These data strongly suggest that arginylation is a dynamic modification of the leading edge actin that is integral to the migration‐dependent signaling network. (Source: Traffic)
Source: Traffic - March 8, 2018 Category: Research Authors: Iuliia Pavlyk, Nicolae A. Leu, Pavan Vedula, Satoshi Kurosaka, Anna Kashina Tags: ORIGINAL ARTICLE Source Type: research
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Traffic, Ahead of Print. (Source: Traffic)
Source: Traffic - March 4, 2018 Category: Research Source Type: research
The Sla1 adaptor ‐clathrin interaction regulates coat formation and progression of endocytosis
This study shows that cells expressing sla1AAA have normal clathrin levels at early stages of endocytosis but reduced clathrin levels at late stages of endocytosis, after the arrival of sla1AAA. Defective Sla1‐clathrin interaction also leads to a delayed progression through stages of endocytosis, especially between the arrival of the Arp2/3 complex activation machinery and the initiation of actin polymerization. Ultimately, an impaired Sla1‐clathrin interaction causes a dramatic increase in overall level of actin and other machinery proteins at sites of endocytosis as well as a longer membrane invagination. (Source: Traffic)
Source: Traffic - March 1, 2018 Category: Research Authors: Thomas O. Tolsma, Lena M. Cuevas, Santiago M. Di Pietro Tags: ORIGINAL ARTICLE Source Type: research
PtdIns(4,5)P2 is not required for secretory granule docking
Phosphoinositides (PtdIns) play important roles in exocytosis and are thought to regulate secretory granule docking by co‐clustering with the SNARE protein syntaxin to form a docking receptor in the plasma membrane. Here we tested this idea by high‐resolution TIRF imaging of EGFP‐labeled PtdIns markers or syntaxin‐1 at secretory granule release sites in live insulin‐secreting cells. In intact cells, PtdIns markers distributed evenly across the plasma membrane with no preference for granule docking sites. In contrast, syntaxin‐1 was found clustered in the plasma membrane, mostly beneath docked granules. We also ...
Source: Traffic - March 1, 2018 Category: Research Authors: Muhmmad Omar ‐Hmeadi, Nikhil Gandasi, Sebastian Barg Tags: ORIGINAL ARTICLE Source Type: research
Blocking Variant Surface Glycoprotein synthesis alters ERES/ Golgi homeostasis in Trypanosoma brucei
The predominant secretory cargo of bloodstream form Trypanosoma brucei is Variant Surface Glycoprotein (VSG), comprising ~10% total protein and forming a dense protective layer. Blocking VSG translation using Morpholino oligonucleotides triggered a precise pre‐cytokinesis arrest. We investigated the effect of blocking VSG synthesis on the secretory pathway. The number of Golgi decreased, particularly in post‐mitotic cells, from 3.5 ± 0.6 to 2.0 ± 0.04 per cell. Similarly, the number of ER exit sites (ERES) in post‐mitotic cells dropped from (3.9 ± 0.6) to (2.7 ± 0.1) eight hours after blocking VSG synthesis. The ...
Source: Traffic - March 1, 2018 Category: Research Authors: Cher ‐Pheng Ooi, Terry K. Smith, Eva Gluenz, Nadina Vasileva Wand, Sue Vaughan, Gloria Rudenko Tags: ORIGINAL ARTICLE Source Type: research
Creating a systematic database of secretory pathway proteins uncovers new cargo for COPI
A third of yeast genes encode for proteins that function in the endomembrane system. However, the precise localization for many of these proteins is still uncertain. Here, we visualized a collection of ~500 N‐terminally, green fluorescent protein (GFP), tagged proteins of the yeast Saccharomyces cerevisiae. By co‐localizing them with seven known markers of endomembrane compartments we determined the localization for over 200 of them. Using this approach, we create a systematic database of the various secretory compartments and identify several new residents. Focusing in, we now suggest that Lam5 resides in contact site...
Source: Traffic - March 1, 2018 Category: Research Authors: Uri Weill, Eric C. Arakel, Omer Goldmann, Matan Golan, Silvia Chuartzman, Sean Munro, Blanche Schwappach, Maya Schuldiner Tags: TOOLBOX Source Type: research
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Traffic,Volume 19, Issue 3, Page 153-155, March 2018. (Source: Traffic)
Source: Traffic - February 26, 2018 Category: Research Source Type: research
Clathrin and AP1 are required for apical sorting of glycosyl phosphatidyl inositol ‐anchored proteins in biosynthetic and recycling routes in Madin‐Darby canine kidney cells
Recently, studies in animal models demonstrate potential roles for clathrin and AP1 in apical protein sorting in epithelial tissue. However, the precise functions of these proteins in apical protein transport remain unclear. Here, we reveal mistargeting of endogenous glycosyl phosphatidyl inositol‐anchored proteins (GPI‐APs) and soluble secretory proteins in Madin‐Darby canine kidney (MDCK) cells upon clathrin heavy chain or AP1 subunit knockdown (KD). Using a novel directional endocytosis and recycling assay, we found that these KD cells are not only affected for apical sorting of GPI‐APs in biosynthetic pathway b...
Source: Traffic - February 26, 2018 Category: Research Authors: Guillaume A. Castillon, Patricia Burriat ‐Couleru, Daniel Abegg, Nina Criado Santos, Reika Watanabe Tags: ORIGINAL ARTICLE Source Type: research
