V1b vasopressin receptor trafficking and signaling: Role of arrestins, G proteins and Src kinase
The signaling pathway of G protein‐coupled receptors is strongly linked to their trafficking profile. Little is known about the molecular mechanisms involved in the vasopressin receptor V1b subtype (V1bR) trafficking and its impact on receptor signaling and regulation. For this purpose, we investigated the role of β‐arrestins in receptor desensitization, internalization and recycling and attempted to dissect the V1bR‐mediated MAP kinase pathway. Using MEF cells Knocked‐out for β‐arrestins 1 and 2, we demonstrated that both β‐arrestins 1 and 2 play a fundamental role in internalization and recycling of V1bR w...
Source: Traffic - December 21, 2017 Category: Research Authors: Sanja Perkovska, Catherine M éjean, Mohammed Akli Ayoub, Juan Li, Floriane Hemery, Maithé Corbani, Nadine Laguette, Maria‐Angeles Ventura, Hélène Orcel, Thierry Durroux, Bernard Mouillac, Christiane Mendre Tags: ORIGINAL ARTICLE Source Type: research
Issue Information
(Source: Traffic)
Source: Traffic - December 21, 2017 Category: Research Tags: ISSUE INFORMATION Source Type: research
Retromer and the cation ‐independent mannose 6‐phosphate receptor—Time for a trial separation?
The retromer cargo‐selective complex (CSC) comprising Vps35, Vps29 and Vps26 mediates the endosome‐to‐Golgi retrieval of the cation‐independent mannose 6‐phosphate receptor (CIMPR). Or does it? Recently published data have questioned the validity of this long‐established theory. Here, the evidence for and against a role for the retromer CSC in CIMPR endosome‐to‐Golgi retrieval is examined in the light of the new data that the SNX‐BAR dimer is actually responsible for CIMPR retrieval.
The cation‐independent mannose 6‐phosphate receptor (CIMPR) has long been regarded as the archetypal cargo protein fo...
Source: Traffic - December 21, 2017 Category: Research Authors: Matthew N. J. Seaman Tags: PERSPECTIVE Source Type: research
The biology and polymer physics underlying large ‐scale chromosome organization
Chromosome large‐scale organization is a beautiful example of the interplay between physics and biology. DNA molecules are polymers and thus belong to the class of molecules for which physicists have developed models and formulated testable hypotheses to understand their arrangement and dynamic properties in solution, based on the principles of polymer physics. Biologists documented and discovered the biochemical basis for the structure, function and dynamic spatial organization of chromosomes in cells. The underlying principles of chromosome organization have recently been revealed in unprecedented detail using high‐r...
Source: Traffic - December 3, 2017 Category: Research Authors: Shelley Sazer, Helmut Schiessel Tags: REVIEW Source Type: research
Cargo crowding at actin ‐rich regions along axons causes local traffic jams
ABSTRACT
Steady axonal cargo flow is central to the functioning of healthy neurons. However, a substantial fraction of cargo in axons remains stationary up to several minutes (Kang, Tian et al. 2008, Tang, Scott et al. 2012, Tang, Scott et al. 2013, Iacobucci, Rahman et al. 2014). We examine the transport of precursors of synaptic vesicles (pre‐SVs), endosomes and mitochondria in C. elegans touch receptor neurons (TRNs), showing that stationary cargo are predominantly present at actin‐rich regions along the neuronal process. Stationary vesicles at actin‐rich regions increase the propensity of moving vesicles to stall...
Source: Traffic - November 28, 2017 Category: Research Authors: Parul Sood, Kausalya Murthy, T. Vinod Kumar, Michael L Nonet, Gautam I. Menon, Sandhya P. Koushika Tags: ORIGINAL ARTICLE Source Type: research
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Traffic,Volume 19, Issue 3, Page 166-181, March 2018. (Source: Traffic)
Source: Traffic - November 27, 2017 Category: Research Source Type: research
Journey to the centre of the cell: Virtual reality immersion into scientific data
In this study, we show how serial block‐face SEM data can be used to build a three‐dimensional (3D) virtual model of a cell that allows scientists, students and members of the public to explore and interact with a “real” cell. (Source: Traffic)
Source: Traffic - November 21, 2017 Category: Research Authors: Angus P.R. Johnston, James Rae, Nicholas Ariotti, Benjamin Bailey, Andrew Lija, Robyn Webb, Charles Ferguson, Sheryl Maher, Thomas P. Davis, Richard I. Webb, John McGhee, Robert G. Parton Tags: Original Article Source Type: research
Distinct features of multivesicular body ‐lysosome fusion revealed by a new cell‐free content‐mixing assay
When marked for degradation, surface receptor and transporter proteins are internalized and delivered to endosomes where they are packaged into intralumenal vesicles (ILVs). Many rounds of ILV formation create multivesicular bodies (MVBs) that fuse with lysosomes exposing ILVs to hydrolases for catabolism. Despite being critical for protein degradation, the molecular underpinnings of MVB‐lysosome fusion remain unclear, although machinery underlying other lysosome fusion events is implicated. But how then is specificity conferred? And how is MVB maturation and fusion coordinated for efficient protein degradation? To addre...
Source: Traffic - November 14, 2017 Category: Research Authors: Mahmoud Abdul Karim, Dieter Ronny Samyn, Sevan Mattie, Christopher Leonard Brett Tags: ORIGINAL ARTICLE Source Type: research
Retromer and the CIMPR – time for a trial separation?
The retromer cargo‐selective complex (CSC) comprising Vps35, Vps29 and Vps26 mediates the endosome‐to‐Golgi retrieval of the cation‐independent mannose 6‐phosphate receptor (CIMPR). Or does it? Recently published data has questioned the validity of this long established theory. Here the evidence for and against a role for the retromer CSC in CIMPR endosome‐to‐Golgi retrieval is examined in the light of the new data that the SNX‐BAR dimer is actually responsible for CIMPR retrieval.
Synopsis
The cation‐independent mannose 6‐phosphate receptor (CIMPR) has long been regarded as the archetypal cargo protei...
Source: Traffic - November 14, 2017 Category: Research Authors: Matthew N.J. Seaman Tags: PERSPECTIVE Source Type: research
Structural insights into the nuclear import of the histone acetyltransferase males ‐absent‐on‐the‐first by importin α1
The histone acetyltransferase males‐absent‐on‐the‐first (MOF) acetylates the histone H4, a modification important for many biological processes, including chromatin organization, transcriptional regulation, DNA replication, recombination and repair, as well as autophagy. Depletion of MOF induces serious consequences because of the reduction of histone acetylation, such as nuclear morphological defects and cancer. Despite the critical roles of MOF in the nucleus, the structural or functional mechanisms of the nucleocytoplasmic transport of MOF remain elusive. Here, we identified novel importin α1‐specific nuclear...
Source: Traffic - November 10, 2017 Category: Research Authors: Weili Zheng, Rui Wang, Xi Liu, Siyu Tian, Benqiang Yao, Ang Chen, Shikai Jin, Yong Li Tags: ORIGINAL ARTICLE Source Type: research
Deciphering lipid codes: K ‐Ras as a paradigm
The cell plasma membrane (PM) is a highly dynamic and heterogeneous lipid environment, driven by complex hydrophobic and electrostatic interactions among the hundreds of types of lipid species. Although the biophysical processes governing lipid lateral segregation in the cell PM have been established in vitro, biological implications of lipid heterogeneity are poorly understood. Of particular interest is how membrane proteins potentially utilize transient spatial clustering of PM lipids to regulate function. The current review focuses on a lipid anchored small GTPase K‐Ras as an example to explore how its C‐terminal me...
Source: Traffic - November 9, 2017 Category: Research Authors: Yong Zhou, John F. Hancock Tags: REVIEW Source Type: research
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Traffic,Volume 19, Issue 3, Page 157-165, March 2018. (Source: Traffic)
Source: Traffic - November 9, 2017 Category: Research Source Type: research
Issue Information
(Source: Traffic)
Source: Traffic - November 8, 2017 Category: Research Tags: ISSUE INFORMATION Source Type: research
TMD1 domain and CRAC motif determine the association and disassociation of MxIRT1 with detergent ‐resistant membranes
Iron is essential for most living organisms. The iron‐regulated transporter1 (IRT1) plays a major role in iron uptake in roots, and its trafficking from endoplasmic reticulum (ER) to plasma membrane (PM) is tightly coordinated with changes in iron environment. However, studies on IRT1 response are limited. Here, we report that Malus xiaojinesis IRT1 (MxIRT1) associates with detergent‐resistant membranes (DRMs, a biochemical counterpart of PM microdomains), whereas the PM microdomains are known platforms for signal transduction in the PM. Depending on the shift of MxIRT1 from microdomains to homogeneous regions in PM, M...
Source: Traffic - November 1, 2017 Category: Research Authors: Song Tan, Peng Zhang, Wei Xiao, Bing Feng, Lan ‐You Chen, Shuang Li, Peng Li, Wei‐Zhong Zhao, Xiao‐Ting Qi, Li‐Ping Yin Tags: ORIGINAL ARTICLE Source Type: research
The Biology and Polymer Physics Underlying Large Scale Chromosome Organization
Chromosome large‐scale organization is a beautiful example of the interplay between physics and biology. DNA molecules are polymers and thus belong to the class of molecules for which physicists have developed models and formulated testable hypotheses to understand their arrangement and dynamic properties in solution, based on the principles of polymer physics. Biologists documented and discovered the biochemical basis for the structure, function and dynamic spatial organization of chromosomes in cells. The underlying principles of chromosome organization have recently been revealed in unprecedented detail using high‐r...
Source: Traffic - November 1, 2017 Category: Research Authors: Shelley Sazer, Helmut Schiessel Tags: REVIEW Source Type: research
