Issue Information
(Source: Traffic)
Source: Traffic - December 22, 2016 Category: Research Tags: Issue Information Source Type: research
The C ‐terminal tails of heterotrimeric kinesin‐2 motor subunits directly bind to α‐tubulin1: possible implications for cilia‐specific tubulin entry
Abstract
The assembly of microtubule‐based cytoskeleton propels the cilia and flagella growth. Previous studies have indicated that the kinesin‐2 family motors transport tubulin into the cilia through intraflagellar transport. Here, we report a direct interaction between the C‐terminal tail fragments of heterotrimeric kinesin‐2 and α‐tubulin1 isoforms in vitro. Blot overlay screen, affinity purification from tissue extracts, cosedimentation with subtilisin‐treated microtubule and LC‐ESI‐MS/MS characterization of the tail‐fragment‐associated tubulin identified an association between the tail domains and...
Source: Traffic - December 14, 2016 Category: Research Authors: Mukul Girotra, Shalini Srivastava, Anuttama Kulkarni, Ayan Barbora, Kratika Bobra, Debnath Ghosal, Pavithra Devan, Amol Aher, Akanksha Jain, Dulal Panda, Krishanu Ray Tags: Original Article Source Type: research
Amyloid precursor protein traffics from the Golgi directly to early endosomes in an Arl5b and AP4 dependent pathway
Abstract
The intracellular trafficking and proteolytic processing of the membrane‐bound amyloid precursor protein (APP) are co‐ordinated events leading to the generation of pathogenic amyloid‐beta (Aβ) peptides. The membrane transport of newly synthesised APP from the Golgi to the endolysosomal system is not well defined, yet is likely to be critical for regulating its processing by β‐secretase (BACE1) and γ‐secretase. Here we show that the majority of newly synthesised APP is transported from the trans‐Golgi network (TGN) directly to early endosomes and then subsequently to the late endosomes/lysosomes with...
Source: Traffic - November 30, 2016 Category: Research Authors: Wei Hong Toh, Jing Zhi A. Tan, Khalisah L. Zulkefli, Fiona J. Houghton, Paul A. Gleeson Tags: Original Article Source Type: research
Targeting of tail ‐anchored membrane proteins to subcellular organelles in Toxoplasma gondii
Abstract
Proper protein localization is essential for critical cellular processes, including vesicle‐mediated transport and protein translocation. Tail‐anchored (TA) proteins are integrated into organellar membranes via the C‐terminus, orienting the N‐terminus towards the cytosol. Localization of TA proteins occurs post‐translationally and is governed by the C‐terminus, which contains the integral transmembrane domain (TMD) and targeting sequence. Targeting of TA proteins is dependent on the hydrophobicity of the TMD as well as the length and composition of flanking amino acid sequences. We previously identifie...
Source: Traffic - November 30, 2016 Category: Research Authors: Leah R. Padgett, Gustavo Arrizabalaga, William J. Sullivan Tags: Original Article Source Type: research
Mitochondrial protein import in trypanosomes ‐ Expect the unexpected
Abstract
Mitochondria have many different functions the most important one of which is oxidative phosphorylation. They originated from an endosymbiotic event between a bacterium and an archaeal host cell. It was the evolution of a protein import system that marks the boundary between the endosymbiotic ancestor of the mitochondrion and a true organelle that is under the control of the nucleus. In present day mitochondria more than 95% of all proteins are imported from the cytosol, in a process mediated by hetero‐oligomeric protein complexes in the outer and inner mitochondrial membranes. In this review we compare mitochon...
Source: Traffic - November 30, 2016 Category: Research Authors: Anke Harsman, Andr é Schneider Tags: Review Source Type: research
Editorial overview: Membrane traffic and cell polarity
(Source: Traffic)
Source: Traffic - November 24, 2016 Category: Research Authors: David M. Bryant, Alpha S. Yap Tags: Editorial Source Type: research
Issue Information
(Source: Traffic)
Source: Traffic - November 24, 2016 Category: Research Tags: Issue Information Source Type: research
Issue Cover (December 2016)
Cover legend: Stitched‐together series of images of polarised MDCK cells grown as 3D cysts. Blue are Nuclei, Green is F‐actin. Red is expression of a protein that disrupts efficient lumen formation. See Roman‐Fernandez and Bryant, Traffic 2016; 17(12):1244–1261.
Read the full article on doi:10.1111/tra.12417 (Source: Traffic)
Source: Traffic - November 24, 2016 Category: Research Tags: Issue Cover Source Type: research
Characterization of a caveolin ‐1 mutation associated with both pulmonary arterial hypertension and congenital generalized lipodystrophy
Congenital generalized lipodystrophy (CGL) and pulmonary arterial hypertension (PAH) have recently been associated with mutations in the caveolin‐1 (
CAV1
) gene, which encodes the primary structural protein of caveolae. However, little is currently known about how these
CAV1
mutations impact caveolae formation or contribute to the development of disease. Here, we identify a heterozygous F160X
CAV1
mutation predicted to generate a C‐terminally truncated mutant protein in a patient with both PAH and CGL using whole exome sequencing, and characterize the properties of
CAV1
, caveolae‐associated proteins and caveol...
Source: Traffic - November 1, 2016 Category: Research Authors: Bing Han, Courtney A. Copeland, Yumeko Kawano, Erika Berman Rosenzweig, Eric D. Austin, Layla Shahmirzadi, Sha Tang, Krishnan Raghunathan, Wendy K. Chung, Anne K. Kenworthy Tags: Case Report Source Type: research
Cargo Selectivity of Yeast Sorting Nexins
ABSTRACT
Sorting nexins are PX domain‐containing proteins that bind phospholipids and often act in membrane trafficking where they help to select cargo. However, the functions and cargo specificities of many sorting nexins are unknown. Here, a high‐throughput imaging screen was used to identify new sorting nexin cargo in the yeast Saccharomyces cerevisiae. Deletions of nine different sorting nexins were screened for mislocalization of a set of GFP‐tagged membrane proteins found at the plasma membrane, Golgi or endosomes. This identified 27 proteins that require one or more sorting nexins for their correct localizatio...
Source: Traffic - October 31, 2016 Category: Research Authors: Bj örn D.M. Bean, Michael Davey, Elizabeth Conibear Tags: Original Article Source Type: research
Cellular and viral peptides bind multiple sites on the N ‐terminal domain of clathrin
We present structures of NTD bound to cellular and viral clathrin‐binding peptide motifs, which unexpectedly all bind to multiple sites on NTD. Biochemical analysis confirms that multiple binding sites are required for efficient capture of NTD by adaptor peptides. Our crystallographic studies also structurally define the proposed ‘fourth’ peptide binding site on NTD. (Source: Traffic)
Source: Traffic - October 31, 2016 Category: Research Authors: Julia Muenzner, Linton M. Traub, Bernard T. Kelly, Stephen C. Graham Tags: Original Article Source Type: research
An evolutionary balance: conservation vs innovation in ciliate membrane trafficking
As most of eukaryotic diversity lies in single‐celled protists, they represent unique opportunities to ask questions about the balance of conservation and innovation in cell biological features. Among free‐living protists the ciliates offer ease of culturing, a rich array of experimental approaches, and versatile molecular tools, particularly in Tetrahymena thermophila and Paramecium tetraurelia. These attributes have been exploited by researchers to analyze a wealth of cellular structures in these large and complex cells. This mini‐review focuses on 3 aspects of ciliate membrane dynamics, all linked with endolysosom...
Source: Traffic - October 26, 2016 Category: Research Authors: Sabrice Guerrier, Helmut Plattner, Elisabeth Richardson, Joel B. Dacks, Aaron P. Turkewitz Tags: Reviews Source Type: research
Issue Information
(Source: Traffic)
Source: Traffic - October 24, 2016 Category: Research Tags: Issue Information Source Type: research
Issue Cover (November 2016)
Cover legend: Newly replicated adenoviral DNA was pulse‐labeled with EdU and detected with fluorescent dye‐conjugated azide after chasing (magenta), while viral DNA replication on compartments were visualized by anti‐USP7 staining (cyan). See Komatsu et al. Traffic 2016; 17(11): 1168–1180.
Read the full article on doi:10.1111/tra.12429 (Source: Traffic)
Source: Traffic - October 24, 2016 Category: Research Tags: Issue Cover Source Type: research
Using HHsearch to tackle proteins of unknown function: A pilot study with PH domains
Advances in membrane cell biology are hampered by the relatively high proportion of proteins with no known function. Such proteins are largely or entirely devoid of structurally significant domain annotations. Structural bioinformaticians have developed profile‐profile tools such as HHsearch (online version called HHpred), which can detect remote homologies that are missed by tools used to annotate databases. Here we have applied HHsearch to study a single structural fold in a single model organism as proof of principle. In the entire clan of protein domains sharing the pleckstrin homology domain fold in yeast, systemati...
Source: Traffic - October 8, 2016 Category: Research Authors: David R. Fidler, Sarah E. Murphy, Katherine Courtis, Pantelis Antonoudiou, Rana El ‐Tohamy, Jonathan Ient, Timothy P. Levine Tags: Toolbox Source Type: research
