Cellular and viral peptides bind multiple sites on the N ‐terminal domain of clathrin

We present structures of NTD bound to cellular and viral clathrin‐binding peptide motifs, which unexpectedly all bind to multiple sites on NTD. Biochemical analysis confirms that multiple binding sites are required for efficient capture of NTD by adaptor peptides. Our crystallographic studies also structurally define the proposed ‘fourth’ peptide binding site on NTD.

Source: Traffic - October 31, 2016 Category: Research Authors: Julia Muenzner, Linton M. Traub, Bernard T. Kelly, Stephen C. Graham Tags: Original Article Source Type: research