Rapid and dynamic arginylation of the leading edge β‐ actin is required for cell migration
This study demonstrates that β‐ actin arginylation occurs primarily at the very edge of migrating cells, that it responds rapidly to cell stimulation inducing migratory behavior, and that its inhibition severely impairs cell migration. These data strongly suggest that arginylation is a dynamic modification of the leading edge actin that is integral to the migration‐dependent signaling network. (Source: Traffic)
Source: Traffic - January 31, 2018 Category: Research Authors: Iuliia Pavlyk, Nicolae A. Leu, Pavan Vedula, Satoshi Kurosaka, Anna Kashina Tags: ORIGINAL ARTICLE Source Type: research
Rapid and dynamic arginylation of the leading edge β‐actin is required for cell migration
Traffic,Volume 19, Issue 4, Page 263-272, April 2018. (Source: Traffic)
Source: Traffic - January 31, 2018 Category: Research Source Type: research
A role for MIRO1 in motility and membrane dynamics of peroxisomes
Abstract
Peroxisomes are dynamic organelles which fulfil essential roles in lipid and ROS metabolism. Peroxisome movement and positioning allows interaction with other organelles and is crucial for their cellular function. In mammalian cells, such movement is microtubule‐dependent and mediated by kinesin and dynein motors. The mechanisms of motor recruitment to peroxisomes are largely unknown, as well as the role this plays in peroxisome membrane dynamics and proliferation. Here, using a combination of microscopy, live‐cell imaging analysis and mathematical modelling, we identify a role for the Ras GTPase MIRO1 as an a...
Source: Traffic - January 24, 2018 Category: Research Authors: In ês Gomes Castro, David Mark Richards, Jeremy Metz, Joseph Liam Costello, Josiah Benjamin Passmore, Tina Angelika Schrader, Ana Gouveia, Daniela Ribeiro, Michael Schrader Tags: ORIGINAL ARTICLE Source Type: research
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Traffic,Volume 19, Issue 3, Page 229-242, March 2018. (Source: Traffic)
Source: Traffic - January 24, 2018 Category: Research Source Type: research
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Traffic,Volume 19, Issue 3, Page 182-197, March 2018. (Source: Traffic)
Source: Traffic - January 23, 2018 Category: Research Source Type: research
Issue Information
(Source: Traffic)
Source: Traffic - January 23, 2018 Category: Research Tags: ISSUE INFORMATION Source Type: research
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Traffic,Volume 19, Issue 3, Page 215-228, March 2018. (Source: Traffic)
Source: Traffic - January 20, 2018 Category: Research Source Type: research
Rab35 GTPase and cancer: Linking membrane trafficking to tumorigenesis
Traffic,Volume 19, Issue 4, Page 247-252, April 2018. (Source: Traffic)
Source: Traffic - January 4, 2018 Category: Research Source Type: research
Dysregulation of Rab5 ‐mediated endocytic pathways in Alzheimer's disease
Traffic,Volume 19, Issue 4, Page 253-262, April 2018. (Source: Traffic)
Source: Traffic - January 3, 2018 Category: Research Source Type: research
Discrimination Between the ER and Mitochondria by Spontaneously Inserting Tail ‐Anchored Proteins
ABSTRACT
Tail‐anchored (TA) proteins insert into their target organelles by incompletely elucidated post‐translational pathways. Some TA proteins spontaneously insert into protein‐free liposomes, yet target a specific organelle in vivo. Two spontaneously inserting cytochrome b5 forms, b5‐ER and b5‐RR, which differ only in the charge of the C‐terminal region, target the ER or the mitochondrial outer membrane (MOM), respectively. To bridge the gap between the cell‐free and in cellula results, we analyzed targeting in digitonin‐permeabilized adherent HeLa cells. In the absence of cytosol the MOM was the destin...
Source: Traffic - January 1, 2018 Category: Research Authors: Bruna Figueiredo Costa, Patrizia Cassella, Sara Francesca Colombo, Nica Borgese Tags: ORIGINAL ARTICLE Source Type: research
Clathrin and AP1 are required for apical sorting of GPI ‐APs in biosynthetic and recycling routes in MDCK cells
Abstract
Recently studies in animal models demonstrate potential roles for clathrin and AP1 in apical protein sorting in epithelial tissue. However, the precise functions of these proteins in apical protein transport remain unclear. Here, we reveal mis‐targeting of endogenous GPI‐APs and soluble secretory proteins in MDCK cells upon clathrin heavy chain or AP1 subunit knockdown (KD). Using a novel directional endocytosis and recycling assay, we found that these KD cells are not only affected for apical sorting of GPI‐APs in biosynthetic pathway but also for their apical recycling and basal to apical transcytosis rout...
Source: Traffic - January 1, 2018 Category: Research Authors: Guillaume Alain Castillon, Patricia Burriat ‐Couleru, Daniel Abegg, Nina Criado Santos, Reika Watanabe Tags: ORIGINAL ARTICLE Source Type: research
Rab35 GTPase and Cancer: Linking membrane trafficking to tumorigenesis
Abstract
Rab35 is a small GTPase that is involved in many cellular processes, including membrane trafficking, cell polarity, lipid homeostasis, immunity, phagocytosis and cytokinesis. Recent studies showed that activating mutations confer Rab35 with oncogenic properties. Conversely downregulation of Rab35 inverts apico‐basal cell polarity and promotes cell migration. Here we review Rab35’s known functions in membrane trafficking and signaling, cell division, and cell migration in cancer cells and discuss the importance of Rab35‐dependent membrane trafficking in cancer progression.
Rab35 is a small GTPase that was re...
Source: Traffic - January 1, 2018 Category: Research Authors: Ronan Shaughnessy, Arnaud Echard Tags: REVIEW Source Type: research
Dysregulation of Rab5 ‐Mediated Endocytic Pathways in Alzheimer's Disease
Abstract
Increasing evidence has pointed to that dysregulation of the endo‐lysosomal system is an early cellular phenotype of pathogenesis for Alzheimer's disease (AD). Rab5, a small GTPase, plays a critical role in mediating these processes. Abnormal overactivation of Rab5 has been observed in post‐mortem brain samples of Alzheimer's patients as well as brain samples of mouse models of AD. Recent genome‐wide association studies of Alzheimer's disease have identified RIN3 (Ras and Rab Interactor 3) as a novel risk factor for the disease. RIN3 that functions as a guanine nucleotide exchange factor for Rab5 may serve a...
Source: Traffic - January 1, 2018 Category: Research Authors: Wei Xu, Fang Fang, Jianqing Ding, Chengbiao Wu Tags: REVIEW Source Type: research
Ergosterol is mainly located in the cytoplasmic leaflet of the yeast plasma membrane
Abstract
Transbilayer lipid asymmetry is a fundamental characteristic of the eukaryotic cell plasma membrane (PM). While PM phospholipid asymmetry is well documented, the transbilayer distribution of PM sterols such as mammalian cholesterol and yeast ergosterol is not reliably known. We now report that sterols are asymmetrically distributed across the yeast PM, with the majority (~80%) located in the cytoplasmic leaflet. By exploiting the sterol‐auxotrophic hem1Δ yeast strain we obtained cells in which endogenous ergosterol was quantitatively replaced with dehydroergosterol (DHE), a closely related fluorescent sterol th...
Source: Traffic - December 28, 2017 Category: Research Authors: Lukasz M. Solanko, David P. Sullivan, Yves Y. Sere, Maria Szomek, Anita Lunding, Katarzyna A. Solanko, Azra Pizovic, Lyubomir D. Stanchev, Thomas G ünther Pomorski, Anant K. Menon, Daniel Wüstner Tags: ORIGINAL ARTICLE Source Type: research
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Traffic,Volume 19, Issue 3, Page 198-214, March 2018. (Source: Traffic)
Source: Traffic - December 28, 2017 Category: Research Source Type: research
