The Sla1 adaptor ‐clathrin interaction regulates coat formation and progression of endocytosis

This study shows that cells expressing sla1AAA have normal clathrin levels at early stages of endocytosis but reduced clathrin levels at late stages of endocytosis, after the arrival of sla1AAA. Defective Sla1‐clathrin interaction also leads to a delayed progression through stages of endocytosis, especially between the arrival of the Arp2/3 complex activation machinery and the initiation of actin polymerization. Ultimately, an impaired Sla1‐clathrin interaction causes a dramatic increase in overall level of actin and other machinery proteins at sites of endocytosis as well as a longer membrane invagination.

Source: Traffic - March 1, 2018 Category: Research Authors: Thomas O. Tolsma, Lena M. Cuevas, Santiago M. Di Pietro Tags: ORIGINAL ARTICLE Source Type: research