Atomic force microscopy study of ionomycin‐induced degranulation in RBL‐2H3 cells
Summary
Mast cell degranulation is the typical anaphylaxis process of mast cells associated with the release of cytokines, eicosanoids and their secretory granules, which play very important roles in the allergic inflammatory response of the human body upon anaphylactogen stimulation. The calcium ionophore ionomycin is widely used as a degranulation induction agent for mast cell degranulation studies. In the present work, ionomycin‐induced degranulation of RBL‐2H3 basophilic leukemia cell line cells was investigated in vitro by high resolution atomic force microscopy (AFM). Ionomycin, which could increase the intracell...
Source: Scanning - November 1, 2015 Category: Radiology Authors: Jiang Pi, Lufen Huang, Fen Yang, Jinhuan Jiang, Hua Jin, Jianxin Liu, Xiaohui Su, Anguo Wu, Huaihong Cai, Peihui Yang, Jiye Cai Tags: Original Article Source Type: research
Microfluidic‐Based Non‐Enzymatic Glycation Enhances Cross‐Linking of Human Scleral Tissue Compared to Conventional Soaking
Summary
We evaluated nano‐structural and chemical changes in human scleral collagen caused by non‐enzymatic glycation using AFM, Raman spectroscopy, and microfluidics. Twenty 8 × 2 mm2 scleral strips (n = 5, each) were divided into four groups of pure sclera tissues (control group) and sclera tissues with incubation (1 hr in BSS and ribose) and preservation (23 hr in 90% ethanol) for 7 days (BSS + DR7 group) and 30 days (BSS + DR30 group) at room temperature, and 7 days in a microfluidic chip (BSS + DR + µF7 group). The BSS + DR7 and BSS + DR30 groups were incubated in a mixture ...
Source: Scanning - November 1, 2015 Category: Radiology Authors: Samjin Choi, Sang Woong Moon, Kyung‐Hyun Jin, Jae‐Ho Shin Tags: Original Article Source Type: research
Cyclic fatigue resistance, torsional resistance, and metallurgical characteristics of V taper 2 and V taper 2H rotary NiTi files
In conclusion, V2H showed higher cyclic fatigue resistance and torsional resistance than V2. The superior properties of V2H could be attributed to the annealing effect and possibly the martensite phase. SCANNING 9999:1–7, 2016. © 2016 Wiley Periodicals, Inc. (Source: Scanning)
Source: Scanning - November 1, 2015 Category: Radiology Authors: S. W. Chang, K. S. Shim, Y. C. Kim, K. K. Jee, Q. Zhu, H. Perinpanayagam, K. Y. Kum Tags: Original Article Source Type: research
Magnetic field sensitivity at 7‐T using dual‐helmholtz transmit‐only coil and 12‐channel receive‐only bended coil
Summary
The purpose of this study was to combine a dual‐Helmholtz (DH) transmit (Tx)‐only coil and 12‐channel receive (Rx)‐only bended phased array (PA) coil to improve the magnetic flux (|B1|) sensitivity in the superior‐to‐inferior (S‐I) direction during human brain magnetic resonance imaging (MRI) at 7‐T. The proposed coil combination was primarily implemented by electromagnetic (EM) simulation and compared with the 16‐leg birdcage coil and 8‐channel PA coil, which are generally used for the Tx‐ and Rx‐only modes, respectively. The optimal coil combinations for the proposed structure were determi...
Source: Scanning - November 1, 2015 Category: Radiology Authors: Kyoung‐Nam Kim, Yeunchul Ryu, Jeung‐Hoon Seo, Young‐Bo Kim Tags: Original Article Source Type: research
Do matrix metalloproteinase inhibitors improve the bond durability of universal dental adhesives?
Summary
The aim of this study was to evaluate the effects of matrix metalloproteinases (MMPs) inhibitors on the microtensile bond strength (μTBS) and the adhesive–dentin interface of two universal dentin bonding agents, Single Bond Universal and All Bond Universal, after 12 months of water storage. Seventy extracted, caries‐free, human third molars were used in this study. Of these, 50 were used for μTBS testing and 20 were used for scanning electron microscopy. The two bonding agents were applied to flat dentin surfaces in five different ways: self‐etch mode, etch‐and‐rinse mode with 37% phosphoric acid, etch...
Source: Scanning - November 1, 2015 Category: Radiology Authors: Neslihan Tekçe, Safa Tuncer, Mustafa Demirci, Sibel Balci Tags: Original Article Source Type: research
Effects of ultrasound, CaCl2 and STPP on the ultrastructure of the milk goat longissimus muscle fiber observed with atomic force microscopy
Summary
The aim of this study was to analyze the effects of ultrasound bath intensity, CaCl2 and sodium tripolyphosphate (STPP) concentration on the ultrastructure of longissimus muscle fiber from milk goats. The sarcomere length was measured by atomic force microscopy. According to the results of AFM images, the sarcomere length is longest when the conditions were an intensity of 100 W ultrasound bath (42.77% increment), a concentration of 300 mM CaCl2 injection (44.68% increment) or 90 mM STPP injection (19.41% increment). Apart from the sarcomere length, the study put forward a potential index (roughness) to repre...
Source: Scanning - November 1, 2015 Category: Radiology Authors: Junpeng Gao, Ying Wang, Lu Liu, Kaiyue Li, Sheqi Zhang, Jie Zhu Tags: Original Article Source Type: research
Radiological, stereological, and microscopic evaluation of the quality of canal fillings in oval‐shaped root canals prepared with self‐adjusting file
Summary
The aim of this study was to evaluate the quality of the canal fillings performed by thermoplastic technique (Herofill) and cold lateral condensation (CLC) techniques in oval shaped canals following self‐adjusting file (SAF) preparation using radiological, stereological, and microscopic evaluation methods. Twelve pairs of oval‐shaped root canals were prepared with SAF and filled using CLC (n = 12) or the Herofill system (n = 12). The quality of the root canal filling was evaluated by (i) scoring bidirectional storage phosphor plate images, (ii) stereological (volumetric) analysis of cone beam computeriz...
Source: Scanning - November 1, 2015 Category: Radiology Authors: Emel Uzunoglu, Can Ilgın, Sinan Yuruker, Melahat Görduysus Tags: Original Article Source Type: research
Detailed Monte Carlo Simulation of electron transport and electron energy loss spectra
Scanning,Volume 38, Issue 6, Page 475-491, November/December 2016. (Source: Scanning)
Source: Scanning - October 29, 2015 Category: Radiology Authors: M. Attarian Shandiz
,
F. Salvat
,
R. Gauvin Source Type: research
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Scanning,Volume 38, Issue 6, Page 475-491, November/December 2016. (Source: Scanning)
Source: Scanning - October 29, 2015 Category: Radiology Source Type: research
Detection of CD28/CD86 co ‐stimulatory molecules and surface properties of T and dendritic cells: An AFM study
Summary
Although the importance of B7/CD28 co‐stimulation has been widely studied, little is known about their nano‐spatial localization and their corresponding cells' biophysical and biomechanical properties. Here, we investigated the morphological, biophysical, and biomechanical properties of T cells and dendritic cells (DCs) by atomic force microscopy (AFM) and force curves. The nano‐spatial distribution of CD28 and CD86 antigen on T cells and DCs was detected by CD86 or CD28 antibody‐functionalized AFM tip. Single‐molecule force spectroscopy (SMFS)‐based force volumes and quantum dots (QDs)‐based fluoresc...
Source: Scanning - October 27, 2015 Category: Radiology Authors: Xun Huang, He Guo, Chuang Wang, Jingjing Mu, Hongxin Zhang, Zhihong Liang, Jiye Cai, Changren Zhou Tags: Original Article Source Type: research
Formation of protective deposits by anti ‐erosive toothpastes—a microscopic study on Enamel with Artificial defects
This study investigated formation of protective deposits on the enamel surface after application of several anti‐erosive toothpastes with different active ingredients. NaF‐containing Sensodyne Pronamel, SnCl2/F‐based Elmex Erosion Protection and calcium phosphate‐based BioRepair Plus Sensitivity Control, SensiShield and Enamel Care toothpastes with claimed anti‐erosive properties were tested. Artificial saliva and Elmex Erosion Protection mouth rinse served as control groups. The toothpastes were applied 30 times by a toothbrush for 2 min per day, mouth rinse for 30 s on polished enamel of thirty five human m...
Source: Scanning - October 22, 2015 Category: Radiology Authors: Pavel Bradna, Radka Vrbova, Vlasta Fialova, Devana Housova, Eva Gojisova Tags: Original Article Source Type: research
Issue Information
(Source: Scanning)
Source: Scanning - October 20, 2015 Category: Radiology Tags: Issue Information Source Type: research
Micro‐computed tomographic assessment of quality of obturation in the apical third with continuous wave vertical compaction and single match taper sized cone obturation techniques
Summary
The aim of the present micro‐computed tomography (Micro‐CT) analysis was to compare the quality of obturation in the apical third of the root with the warm vertical compaction (WVC) and single matching taper sized cone (SMTSC) techniques. Mesial roots of 16 freshly extracted mandibular first molar teeth (with a total of 32 canals) were used. Canals in each mesial root were shaped to a size F3 Protaper and were randomly assigned to either continuous wave vertical compaction or single match tapered size cone technique. Volume of voids and gaps in the obturated roots were measured using micro‐CT scanning at 1, 3...
Source: Scanning - October 7, 2015 Category: Radiology Authors: Mohammed Alshehri, Hadi M. Alamri, Emad Alshwaimi, Omar Kujan Tags: Original Article Source Type: research
