Local dynamic range compensation for scanning electron microscope imaging system by sub ‐blocking multiple peak HE with convolution
Scanning,Volume 38, Issue 6, Page 492-501, November/December 2016. (Source: Scanning)
Source: Scanning - November 30, 2015 Category: Radiology Source Type: research

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Scanning,Volume 38, Issue 6, Page 502-514, November/December 2016. (Source: Scanning)
Source: Scanning - November 30, 2015 Category: Radiology Source Type: research

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Scanning,Volume 38, Issue 6, Page 492-501, November/December 2016. (Source: Scanning)
Source: Scanning - November 30, 2015 Category: Radiology Source Type: research

Signal‐to‐noise ratio estimation using adaptive tuning on the piecewise cubic Hermite interpolation model for images
Summary An improvement to the existing technique of quantifying signal‐to‐noise ratio (SNR) of scanning electron microscope (SEM) images using piecewise cubic Hermite interpolation (PCHIP) technique is proposed. The new technique uses an adaptive tuning onto the PCHIP, and is thus named as ATPCHIP. To test its accuracy, 70 images are corrupted with noise and their autocorrelation functions are then plotted. The ATPCHIP technique is applied to estimate the uncorrupted noise‐free zero offset point from a corrupted image. Three existing methods, the nearest neighborhood, first order interpolation and original PCHIP, are...
Source: Scanning - November 30, 2015 Category: Radiology Authors: K. S. Sim, Z. X. Yeap, C. P. Tso Tags: Review Source Type: research

Signal ‐to‐noise ratio estimation using adaptive tuning on the piecewise cubic Hermite interpolation model for images
Summary An improvement to the existing technique of quantifying signal‐to‐noise ratio (SNR) of scanning electron microscope (SEM) images using piecewise cubic Hermite interpolation (PCHIP) technique is proposed. The new technique uses an adaptive tuning onto the PCHIP, and is thus named as ATPCHIP. To test its accuracy, 70 images are corrupted with noise and their autocorrelation functions are then plotted. The ATPCHIP technique is applied to estimate the uncorrupted noise‐free zero offset point from a corrupted image. Three existing methods, the nearest neighborhood, first order interpolation and original PCHIP, are...
Source: Scanning - November 29, 2015 Category: Radiology Authors: K. S. Sim, Z. X. Yeap, C. P. Tso Tags: Review Source Type: research

Local dynamic range compensation for scanning electron microscope imaging system by sub ‐blocking multiple peak HE with convolution
Summary This paper introduces new development technique to improve the Scanning Electron Microscope (SEM) image quality and we name it as sub‐blocking multiple peak histogram equalization (SUB‐B‐MPHE) with convolution operator. By using this new proposed technique, it shows that the new modified MPHE performs better than original MPHE. In addition, the sub‐blocking method consists of convolution operator which can help to remove the blocking effect for SEM images after applying this new developed technique. Hence, by using the convolution operator, it effectively removes the blocking effect by properly distributing...
Source: Scanning - November 29, 2015 Category: Radiology Authors: K. S. Sim, V. Teh, Y. C. Tey, T. K. Kho Tags: Original Article Source Type: research

Evaluation of the bond strength of different adhesive agents to a resin ‐modified calcium silicate material (TheraCal LC)
This study evaluated the bond strength of different adhesive agents to TheraCal LC and mineral trioxide aggregate (MTA) and examined the morphologic changes of these materials with different surface treatments. A total of 120 specimens, 60 of MTA Angelus (AMTA), and 60 of TheraCal LC, were prepared and divided into six subgroups according to the adhesive agent used; these agents included Scotchbond Multipurpose, Clearfil SE Bond, Clearfil Protect Bond, Clearfil S3 Bond, OptiBond All‐in‐One, and G‐aenial Bond. After application of adhesive agents, Filtek Z250 composite resin was placed onto the specimens. Shear bond s...
Source: Scanning - November 9, 2015 Category: Radiology Authors: Muhammed Karadas, Kenan Cantekin, Husniye Gumus, Sabit Melih Ate ş, Zeynep Yesil Duymuş Tags: Original Article Source Type: research

Botulinum neurotoxin effects on masseter muscle fibre in WNIN obese rats—Scanning electron microscope analysis
Summary WNIN/Ob obese mutant rats are unique in comparison to similar rodent models of obesity established in the West. The present study is aimed to evaluate the masticatory function and histological changes in masseter muscle fibres treated with botulinum toxin type A (BoNT/A) in WNIN/Ob rats. Twelve WNIN/Ob obese rats and 12 lean rats at 35 days of age were taken and divided into four groups (6 rats in each group): Group‐I (WNIN/Ob) and Group‐II (lean) rats were injected with BoNT/A (1 unit) into right side of masseter muscle. For control left masseter of both phenotypes was injected with saline. Group‐III (WNIN/O...
Source: Scanning - November 5, 2015 Category: Radiology Authors: Shivaram Nemani, Uday K. Putchha, Madhusudhanachary Periketi, Sailaja Pothana, Giridharan Nappanveettil, Harishankar Nemani Tags: Original Article Source Type: research

Botulinum neurotoxin effects on masseter muscle fibre in WNIN obese rats —Scanning electron microscope analysis
Summary WNIN/Ob obese mutant rats are unique in comparison to similar rodent models of obesity established in the West. The present study is aimed to evaluate the masticatory function and histological changes in masseter muscle fibres treated with botulinum toxin type A (BoNT/A) in WNIN/Ob rats. Twelve WNIN/Ob obese rats and 12 lean rats at 35 days of age were taken and divided into four groups (6 rats in each group): Group‐I (WNIN/Ob) and Group‐II (lean) rats were injected with BoNT/A (1 unit) into right side of masseter muscle. For control left masseter of both phenotypes was injected with saline. Group‐III (WNIN/O...
Source: Scanning - November 4, 2015 Category: Radiology Authors: Shivaram Nemani, Uday K. Putchha, Madhusudhanachary Periketi, Sailaja Pothana, Giridharan Nappanveettil, Harishankar Nemani Tags: Original Article Source Type: research

The effects of cavity ‐filling techniques on microleakage in class II resin restorations prepared with Er:YAG laser and diamond bur: A scanning electron microscopy study
Summary The purpose of this study was to investigate how two cavity‐filling techniques affect microleakage in class II resin restorations prepared with Er:YAG laser and diamond bur. Standard MO and DO cavities were prepared in 20 extracted third molars, each randomly assigned to either Group‐1 [Herculite XRV Ultra‐bur‐prepared cavity(bp)], Group‐2 [Herculite XRV Ultra‐laser‐prepared cavity(lp)], Group‐3 (SonicFill‐(bp)], or Group‐4 [SonicFill‐(lp)]. For Groups 2 and 4, cavities were prepared by using an Er:YAG laser with a wavelength of 2.94 μm, output power of 200 mJ/pulse, and repetition rate o...
Source: Scanning - November 4, 2015 Category: Radiology Authors: Emre Ozel, Elif Bahar Tuna, Erhan Firatli Tags: Original Article Source Type: research

Morphological and nanomechanical changes in mechanosensitive endothelial cells induced by colloidal AFM probes
This article presents examples of how the colloidal AFM probes influence ECs during multiple scans. The results revealed that multiple scans of the ECs significantly influenced the morphology and elasticity of cells. Moreover, changes in the cell shape and mechanical properties were dependent on the scan direction (across or along the main axis of the cell). Multiple scans with a colloidal probe leaded to reorientation of the cell main axis and this effect was similar to the action of the shear stress induced by flow conditions. Furthermore, the correlation between the tip‐induced modification of the cell properties and ...
Source: Scanning - November 1, 2015 Category: Radiology Authors: Marta Targosz‐Korecka, Katarzyna E. Malek‐Zietek, Grzegorz D. Brzezinka, Magdalena Jaglarz Tags: Original Article Source Type: research

Suppression of noise in SEM images using weighted local hysteresis smoothing filter
Summary It has been proven that Hysteresis Smoothing (HS) has several advantages for Scanning Electron Microscopy (SEM) image noise reduction. HS uses hysteresis thresholding to remove noise besides preserving important details of images. Determination of optimal threshold values (cursor width) plays an effective role in improving the performance of HS based filters. Recently, a novel local technique, named Local Adaptive Hysteresis Smoothing (LAHS), has been proposed to compute an optimal cursor width. In this paper, a new method is proposed to improve the performance of LAHS in noise reduction and detail preservation. In...
Source: Scanning - November 1, 2015 Category: Radiology Authors: Mohadeseh Mazhari, Reza P. R. Hasanzadeh Tags: Original Article Source Type: research

Study of SEM preparation artefacts with correlative microscopy: Cell shrinkage of adherent cells by HMDS‐drying
Summary One of the often reported artefacts during cell preparation to scanning electron microscopy (SEM) is the shrinkage of cellular objects, that mostly occurs at a certain time‐dependent stage of cell drying. Various methods of drying for SEM, such as critical point drying, freeze–drying, as well as hexamethyldisilazane (HMDS)‐drying, were usually used. The latter becomes popular since it is a low cost and fast method. However, the correlation of drying duration and real shrinkage of objects was not investigated yet. In this paper, cell shrinkage at each stage of preparation for SEM was studied. We introduce a sh...
Source: Scanning - November 1, 2015 Category: Radiology Authors: Alisa Katsen‐Globa, Norbert Puetz, Michael M. Gepp, Julia C. Neubauer, Heiko Zimmermann Tags: Original Article Source Type: research

An extended simultaneous algebraic reconstruction technique (E‐SART) for X‐ray dual spectral computed tomography
Summary X‐ray dual spectral computed tomography (DSCT) scans the measured object with two different x‐ray energy spectra and the collected polychromatic projections from this procedure can be used to perform basis material decomposition of the object. An iterative method E‐ART was recently proposed to produce highly quantitative basis material images for DSCT, but it has the drawback of slow convergence and huge computational costs. Inspired by the E‐ART method, this paper proposes an extended simultaneous algebraic reconstruction technique (E‐SART) for the basis material decomposition of DSCT and an accelerating...
Source: Scanning - November 1, 2015 Category: Radiology Authors: Jingjing Hu, Xing Zhao, Feng Wang Tags: Original Article Source Type: research

Studying of cellular interaction of hairpin‐like peptide EcAMP1 from barnyard grass (Echinochloa crusgalli L.) seeds with plant pathogenic fungus Fusarium solani using microscopy techniques
Summary An interaction of recombinant hairpin‐like cationic peptide EcAMP1 with conidia of plant pathogenic fungus Fusarium solani at the cellular level was studied by a combination of microscopic methods. EcAMP1 is from barnyard grass (Echinochloa crusgalli L.), and obtained by heterologous expression in Escherichia coli system. As a result, a direct relationship between hyphal growth inhibition and increasing active peptide concentration, time of incubation and fungal physiological condition has been determined. Dynamics of accumulation and redistribution of the peptide studied on fungal cellular cover and inside the c...
Source: Scanning - November 1, 2015 Category: Radiology Authors: Alexey S. Vasilchenko, Mikhail Yuryev, Dmitry Yu Ryazantsev, Sergey K. Zavriev, Alexey V. Feofanov, Eugene V. Grishin, Eugene A. Rogozhin Tags: Original Article Source Type: research